Real-Time Optical Antimicrobial Susceptibility Testing

Marlene Fredborg, Klaus R Andersen, Erik Jørgensen, Aida Droce, Tom Olesen, Bent B Jensen, Flemming S Rosenvinge, Teis E Sondergaard

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Rapid antibiotic susceptibility testing is in highly demand in health-care fields as antimicrobial resistant bacterial strains emerge and spread. Here we describe an optical screening system (oCelloScope), which based on time-lapse imaging of 96 bacteria-antibiotic combinations at a time, introduces real-time detection of bacterial growth and antimicrobial susceptibility, with imaging material to support the automatically generated graphs. Automated antibiotic susceptibility tests of a monoculture showed statistically significant antibiotic effect within 6 minutes and within 30 minutes in complex samples from pigs suffering from catheter associated urinary tract infections. The oCelloScope system provides a fast high-throughput screening method to detect bacterial susceptibility that may entail earlier diagnosis and introduction of appropriate targeted therapy and thus combat the threat from multidrug-resistant pathogenic bacteria. The oCelloScope system can be employed for a broad range of applications within bacteriology and may present new vistas as a point-of-care instrument in both clinical and veterinarian settings.
OriginalsprogEngelsk
TidsskriftJournal of Clinical Microbiology
Vol/bind51
Udgave nummer7
Sider (fra-til)2047-53
ISSN0095-1137
DOI
StatusUdgivet - 2013

Fingeraftryk

Point-of-Care Systems
High-Throughput Screening Assays
Optical Devices
Veterinarians
Delivery of Health Care
Growth

Citer dette

Fredborg, M., Andersen, K. R., Jørgensen, E., Droce, A., Olesen, T., Jensen, B. B., ... Sondergaard, T. E. (2013). Real-Time Optical Antimicrobial Susceptibility Testing. Journal of Clinical Microbiology, 51(7), 2047-53. https://doi.org/10.1128/JCM.00440-13
Fredborg, Marlene ; Andersen, Klaus R ; Jørgensen, Erik ; Droce, Aida ; Olesen, Tom ; Jensen, Bent B ; Rosenvinge, Flemming S ; Sondergaard, Teis E. / Real-Time Optical Antimicrobial Susceptibility Testing. I: Journal of Clinical Microbiology. 2013 ; Bind 51, Nr. 7. s. 2047-53.
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abstract = "Rapid antibiotic susceptibility testing is in highly demand in health-care fields as antimicrobial resistant bacterial strains emerge and spread. Here we describe an optical screening system (oCelloScope), which based on time-lapse imaging of 96 bacteria-antibiotic combinations at a time, introduces real-time detection of bacterial growth and antimicrobial susceptibility, with imaging material to support the automatically generated graphs. Automated antibiotic susceptibility tests of a monoculture showed statistically significant antibiotic effect within 6 minutes and within 30 minutes in complex samples from pigs suffering from catheter associated urinary tract infections. The oCelloScope system provides a fast high-throughput screening method to detect bacterial susceptibility that may entail earlier diagnosis and introduction of appropriate targeted therapy and thus combat the threat from multidrug-resistant pathogenic bacteria. The oCelloScope system can be employed for a broad range of applications within bacteriology and may present new vistas as a point-of-care instrument in both clinical and veterinarian settings.",
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Fredborg, M, Andersen, KR, Jørgensen, E, Droce, A, Olesen, T, Jensen, BB, Rosenvinge, FS & Sondergaard, TE 2013, 'Real-Time Optical Antimicrobial Susceptibility Testing', Journal of Clinical Microbiology, bind 51, nr. 7, s. 2047-53. https://doi.org/10.1128/JCM.00440-13

Real-Time Optical Antimicrobial Susceptibility Testing. / Fredborg, Marlene; Andersen, Klaus R; Jørgensen, Erik; Droce, Aida; Olesen, Tom; Jensen, Bent B; Rosenvinge, Flemming S; Sondergaard, Teis E.

I: Journal of Clinical Microbiology, Bind 51, Nr. 7, 2013, s. 2047-53.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

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AU - Fredborg, Marlene

AU - Andersen, Klaus R

AU - Jørgensen, Erik

AU - Droce, Aida

AU - Olesen, Tom

AU - Jensen, Bent B

AU - Rosenvinge, Flemming S

AU - Sondergaard, Teis E

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AB - Rapid antibiotic susceptibility testing is in highly demand in health-care fields as antimicrobial resistant bacterial strains emerge and spread. Here we describe an optical screening system (oCelloScope), which based on time-lapse imaging of 96 bacteria-antibiotic combinations at a time, introduces real-time detection of bacterial growth and antimicrobial susceptibility, with imaging material to support the automatically generated graphs. Automated antibiotic susceptibility tests of a monoculture showed statistically significant antibiotic effect within 6 minutes and within 30 minutes in complex samples from pigs suffering from catheter associated urinary tract infections. The oCelloScope system provides a fast high-throughput screening method to detect bacterial susceptibility that may entail earlier diagnosis and introduction of appropriate targeted therapy and thus combat the threat from multidrug-resistant pathogenic bacteria. The oCelloScope system can be employed for a broad range of applications within bacteriology and may present new vistas as a point-of-care instrument in both clinical and veterinarian settings.

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Fredborg M, Andersen KR, Jørgensen E, Droce A, Olesen T, Jensen BB et al. Real-Time Optical Antimicrobial Susceptibility Testing. Journal of Clinical Microbiology. 2013;51(7):2047-53. https://doi.org/10.1128/JCM.00440-13