Rainbow trout (Oncorhynchus mykiss) immune response towards a recombinant vaccine targeting the parasitic ciliate Ichthyophthirius multifiliis

L. von Gersdorff Jørgensen*, P. W. Kania, K. J. Rasmussen, A. H. Mattsson, J. Schmidt, A. Al-Jubury, A. Sander, A. Salanti, K. Buchmann

*Kontaktforfatter for dette arbejde

Publikation: Bidrag til tidsskriftTidsskriftartikel

Abstrakt

The protective effect in rainbow trout (Oncorhynchus mykiss) of an experimental subunit vaccine targeting antigens in the parasite Ichthyophthirius multifiliis has been evaluated and compared to effects elicited by a classical parasite homogenate vaccine. Three recombinant parasite proteins (two produced in E. coli and one in insect cells) were combined and injected i.p., and subsequently, protection and antibody responses were analysed. Both the experimental and the benchmark vaccine induced partial but significant protection against I. multifiliis when compared to control fish. Specific antibody responses of vaccinated trout (subunit vaccine) were raised against one neurohypophysial n-terminal domain protein #10 of three recombinant proteins, whereas the benchmark vaccine group showed specific antibody production against all three recombinant proteins. The immunogenic parasite protein #10 may be a potential vaccine candidate supplementing the protective I-antigen in future vaccine trials.

OriginalsprogEngelsk
TidsskriftJournal of Fish Diseases
Vol/bind40
Udgave nummer12
Sider (fra-til)1815-1821
ISSN0140-7775
DOI
StatusUdgivet - 2017

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  • Citationsformater

    von Gersdorff Jørgensen, L., Kania, P. W., Rasmussen, K. J., Mattsson, A. H., Schmidt, J., Al-Jubury, A., Sander, A., Salanti, A., & Buchmann, K. (2017). Rainbow trout (Oncorhynchus mykiss) immune response towards a recombinant vaccine targeting the parasitic ciliate Ichthyophthirius multifiliis. Journal of Fish Diseases, 40(12), 1815-1821. https://doi.org/10.1111/jfd.12653