Radiofrequency encoded angular-resolved light scattering

Brandon W. Buckley, Najva Akbari, Eric D. Diebold, Jost Adam, Bahram Jalali

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Resumé

The sensitive, specific, and label-free classification of microscopic cells and organisms is one of the outstanding problems in biology. Today, instruments such as the flow cytometer use a combination of light scatter measurements at two distinct angles to infer the size and internal complexity of cells at rates of more than 10,000 per second. However, by examining the entire angular light scattering spectrum it is possible to classify cells with higher resolution and specificity. Current approaches to performing these angular spectrum measurements all have significant throughput limitations, making them incompatible with other state-of-the-art flow cytometers. Here, we introduce a method for performing complete angular scattering spectrum measurements at high throughput combining techniques from the field of scattering flow-cytometry and radiofrequency communications. Termed Radiofrequency Encoded Angular-resolved Light Scattering (REALS), this technique multiplexes angular light scattering in the radiofrequency domain, such that a single photodetector captures the entire scattering spectrum from a particle over approximately 100 discrete incident angles on a single shot basis. As a proof-of-principle experiment, we use this technique to perform scattering measurements over a range of 30 degrees from a tapered optical fiber at a scan rate of 250 kHz.
OriginalsprogEngelsk
Artikelnummer123701
TidsskriftApplied Physics Letters
Vol/bind106
Udgave nummer12
Antal sider4
ISSN0003-6951
DOI
StatusUdgivet - 23. mar. 2015

Fingeraftryk

light scattering
scattering
cells
cytometry
biology
organisms
shot
photometers
optical fibers
communication
high resolution

Citer dette

Buckley, B. W., Akbari, N., Diebold, E. D., Adam, J., & Jalali, B. (2015). Radiofrequency encoded angular-resolved light scattering. Applied Physics Letters, 106(12), [123701]. https://doi.org/10.1063/1.4915621
Buckley, Brandon W. ; Akbari, Najva ; Diebold, Eric D. ; Adam, Jost ; Jalali, Bahram. / Radiofrequency encoded angular-resolved light scattering. I: Applied Physics Letters. 2015 ; Bind 106, Nr. 12.
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Buckley, BW, Akbari, N, Diebold, ED, Adam, J & Jalali, B 2015, 'Radiofrequency encoded angular-resolved light scattering', Applied Physics Letters, bind 106, nr. 12, 123701. https://doi.org/10.1063/1.4915621

Radiofrequency encoded angular-resolved light scattering. / Buckley, Brandon W. ; Akbari, Najva; Diebold, Eric D.; Adam, Jost; Jalali, Bahram.

I: Applied Physics Letters, Bind 106, Nr. 12, 123701, 23.03.2015.

Publikation: Bidrag til tidsskriftLetterForskningpeer review

TY - JOUR

T1 - Radiofrequency encoded angular-resolved light scattering

AU - Buckley, Brandon W.

AU - Akbari, Najva

AU - Diebold, Eric D.

AU - Adam, Jost

AU - Jalali, Bahram

PY - 2015/3/23

Y1 - 2015/3/23

N2 - The sensitive, specific, and label-free classification of microscopic cells and organisms is one of the outstanding problems in biology. Today, instruments such as the flow cytometer use a combination of light scatter measurements at two distinct angles to infer the size and internal complexity of cells at rates of more than 10,000 per second. However, by examining the entire angular light scattering spectrum it is possible to classify cells with higher resolution and specificity. Current approaches to performing these angular spectrum measurements all have significant throughput limitations, making them incompatible with other state-of-the-art flow cytometers. Here, we introduce a method for performing complete angular scattering spectrum measurements at high throughput combining techniques from the field of scattering flow-cytometry and radiofrequency communications. Termed Radiofrequency Encoded Angular-resolved Light Scattering (REALS), this technique multiplexes angular light scattering in the radiofrequency domain, such that a single photodetector captures the entire scattering spectrum from a particle over approximately 100 discrete incident angles on a single shot basis. As a proof-of-principle experiment, we use this technique to perform scattering measurements over a range of 30 degrees from a tapered optical fiber at a scan rate of 250 kHz.

AB - The sensitive, specific, and label-free classification of microscopic cells and organisms is one of the outstanding problems in biology. Today, instruments such as the flow cytometer use a combination of light scatter measurements at two distinct angles to infer the size and internal complexity of cells at rates of more than 10,000 per second. However, by examining the entire angular light scattering spectrum it is possible to classify cells with higher resolution and specificity. Current approaches to performing these angular spectrum measurements all have significant throughput limitations, making them incompatible with other state-of-the-art flow cytometers. Here, we introduce a method for performing complete angular scattering spectrum measurements at high throughput combining techniques from the field of scattering flow-cytometry and radiofrequency communications. Termed Radiofrequency Encoded Angular-resolved Light Scattering (REALS), this technique multiplexes angular light scattering in the radiofrequency domain, such that a single photodetector captures the entire scattering spectrum from a particle over approximately 100 discrete incident angles on a single shot basis. As a proof-of-principle experiment, we use this technique to perform scattering measurements over a range of 30 degrees from a tapered optical fiber at a scan rate of 250 kHz.

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