Quantitative proteomics by amino acid labeling in C. elegans

Julius Fredens; Kasper Engholm-Keller; Anders Giessing; Dennis Pultz; Martin Røssel Larsen; Peter Højrup; Jakob Møller-Jensen; Nils J Færgeman

doi:10.1038/nmeth.1675

Quantitative proteomics by amino acid labeling in C. elegans

Julius Fredens, Kasper Engholm-Keller, Anders Giessing, Dennis Pultz, Martin Røssel Larsen, Peter Højrup, Jakob Møller-Jensen, Nils J Færgeman

Institut for Biokemi og Molekylær Biologi

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

Abstrakt

We demonstrate labeling of Caenorhabditis elegans with heavy isotope-labeled lysine by feeding them with heavy isotope-labeled Escherichia coli. Using heavy isotope-labeled worms and quantitative proteomics methods, we identified several proteins that are regulated in response to loss or RNAi-mediated knockdown of the nuclear hormone receptor 49 in C. elegans. The combined use of quantitative proteomics and selective gene knockdown is a powerful tool for C. elegans biology.

Originalsprog	Engelsk
Tidsskrift	Nature Methods
Vol/bind	8
Udgave nummer	10
Sider (fra-til)	845-847
ISSN	1548-7091
DOI	https://doi.org/10.1038/nmeth.1675
Status	Udgivet - 2011

Dokumenter og links

10.1038/nmeth.1675

Molecular Genetics and Quantitative Proteomics to Identify Novel Regulators of Metabolism
Julius Fredens (Oplægsholder)
14. nov. 2011
Aktivitet: Foredrag og mundtlige bidrag › Foredrag og præsentationer i privat eller offentlig virksomhed
A highly efficient strategy for metabolic studies: quantitative proteomics in C. elegans
Julius Fredens (Foredragsholder)
8. sep. 2011
Aktivitet: Foredrag og mundtlige bidrag › Foredrag og præsentationer i privat eller offentlig virksomhed

Citationsformater

@article{29f622afd1fc46d6ab5fb471efa4be40,

title = "Quantitative proteomics by amino acid labeling in C. elegans",

abstract = "We demonstrate labeling of Caenorhabditis elegans with heavy isotope-labeled lysine by feeding them with heavy isotope-labeled Escherichia coli. Using heavy isotope-labeled worms and quantitative proteomics methods, we identified several proteins that are regulated in response to loss or RNAi-mediated knockdown of the nuclear hormone receptor 49 in C. elegans. The combined use of quantitative proteomics and selective gene knockdown is a powerful tool for C. elegans biology.",

author = "Julius Fredens and Kasper Engholm-Keller and Anders Giessing and Dennis Pultz and Larsen, {Martin R{\o}ssel} and Peter H{\o}jrup and Jakob M{\o}ller-Jensen and F{\ae}rgeman, {Nils J}",

year = "2011",

doi = "10.1038/nmeth.1675",

language = "English",

volume = "8",

pages = "845--847",

journal = "Nature Methods",

issn = "1548-7091",

publisher = "Nature Publishing Group",

number = "10",

}

TY - JOUR

T1 - Quantitative proteomics by amino acid labeling in C. elegans

AU - Fredens, Julius

AU - Engholm-Keller, Kasper

AU - Giessing, Anders

AU - Pultz, Dennis

AU - Larsen, Martin Røssel

AU - Højrup, Peter

AU - Møller-Jensen, Jakob

AU - Færgeman, Nils J

PY - 2011

Y1 - 2011

N2 - We demonstrate labeling of Caenorhabditis elegans with heavy isotope-labeled lysine by feeding them with heavy isotope-labeled Escherichia coli. Using heavy isotope-labeled worms and quantitative proteomics methods, we identified several proteins that are regulated in response to loss or RNAi-mediated knockdown of the nuclear hormone receptor 49 in C. elegans. The combined use of quantitative proteomics and selective gene knockdown is a powerful tool for C. elegans biology.

AB - We demonstrate labeling of Caenorhabditis elegans with heavy isotope-labeled lysine by feeding them with heavy isotope-labeled Escherichia coli. Using heavy isotope-labeled worms and quantitative proteomics methods, we identified several proteins that are regulated in response to loss or RNAi-mediated knockdown of the nuclear hormone receptor 49 in C. elegans. The combined use of quantitative proteomics and selective gene knockdown is a powerful tool for C. elegans biology.

U2 - 10.1038/nmeth.1675

DO - 10.1038/nmeth.1675

M3 - Journal article

C2 - 21874006

VL - 8

SP - 845

EP - 847

JO - Nature Methods

JF - Nature Methods

SN - 1548-7091

IS - 10

ER -

Quantitative proteomics by amino acid labeling in C. elegans

Abstrakt

Dokumenter og links

Fingeraftryk

Relaterede Aktiviteter

Molecular Genetics and Quantitative Proteomics to Identify Novel Regulators of Metabolism

A highly efficient strategy for metabolic studies: quantitative proteomics in C. elegans

Citationsformater