Quantitative proteomic study reveals differential expression of matricellular proteins between fibrous dysplasia and cemento-ossifying fibroma pathogenesis

Filipe Fideles Duarte-Andrade, Thais dos Santos Fontes Pereira, Jéssica Gardone Vitório, Marina Gonçalves Diniz, Larissa Stefhanne Damasceno Amorim, Arkadiusz Nawrocki, Liza Figueiredo Felicori, Luiz De Marco, Carolina Cavaliéri Gomes, Martin R. Larsen, Marcella Nunes Melo-Braga, Ricardo Santiago Gomez*

*Kontaktforfatter for dette arbejde

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review


Background: Fibrous dysplasia (FD) and cemento-ossifying fibroma (COF) are the most common gnathic fibro-osseous lesions. These diseases exhibit remarkable overlap of several clinicopathological aspects, and differential diagnosis depends on the combination of histopathological, radiographic, and clinical aspects. Their molecular landscape remains poorly characterized, and herein, we assessed their proteomic and phosphoproteomic profiles. Methods: The quantitative differences in protein profile of FD and COF were assessed by proteomic and phosphoproteomic analyses of formalin-fixed paraffin-embedded tissue samples. Pathway enrichment analyses with differentially regulated proteins were performed. Results: FD and COF exhibited differential regulation of pathways related to extracellular matrix organization, cell adhesion, and platelet and erythrocytes activities. Additionally, these lesions demonstrated distinct abundance of proteins involved in osteoblastic differentiation and tumorigenesis and differential abundance of phosphorylation of Ser61 of Yes-associated protein 1 (YAP1). Conclusions: In summary, despite the morphological similarity between these diseases, our results demonstrated that COF and DF present numerous quantitative differences in their proteomic profiles. These findings suggest that these fibro-osseous lesions trigger distinct molecular mechanisms during their pathogenesis. Moreover, some proteins identified in our analysis could serve as potential biomarkers for differential diagnosis of these diseases after further validation.

TidsskriftJournal of Oral Pathology and Medicine
Udgave nummer4
Sider (fra-til)405-412
StatusUdgivet - apr. 2022

Bibliografisk note

Funding Information:
This study was financed in part by the Coordination for the Improvement of Higher Education Personnel (CAPES)/Brazil, Finance code 001, National Council for Scientific and Technological Development (CNPq), and The Minas Gerais Research Funding Foundation (FAPEMIG). FFD‐A, MNM‐B, and TSFP received CAPES scholarship, and JGV received CNPq scholarship. CCG and RSG are research fellows at CNPq. The VILLUM Center for Bioanalytical Sciences at the University of Southern Denmark is acknowledged for access to state‐of‐the‐art Mass spectrometric instrumentation. Thanks to all members from Protein Research Group at University of Southern Denmark, in special Andrea Maria Lorentzen, Vladimir Gorshkov, and Vibeke Jørgensen for column and instruments support.

Publisher Copyright:
© 2022 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd


Dyk ned i forskningsemnerne om 'Quantitative proteomic study reveals differential expression of matricellular proteins between fibrous dysplasia and cemento-ossifying fibroma pathogenesis'. Sammen danner de et unikt fingeraftryk.