@article{61514f50db4411dc860c000ea68e967b,
title = "Quantitative proteomic assessment of very early cellular signaling events",
abstract = "Technical limitations have prevented proteomic analyses of events occurring less than 30 s after signal initiation. We developed an automated, continuous quench-flow system allowing quantitative proteomic assessment of very early cellular signaling events (qPACE) with a time resolution of 1 s. Using this technique, we determined that autophosphorylation of the epidermal growth factor receptor occurs within 1 s after ligand stimulation and is followed rapidly by phosphorylation of the downstream signaling intermediates Src homologous and collagen-like protein and phospholipase C gamma 1.",
keywords = "Fluorescence Recovery After Photobleaching, Metabolic Clearance Rate, Microscopy, Fluorescence, Phosphorylation, Proteome, Receptor, Epidermal Growth Factor, Signal Transduction, Time Factors",
author = "Joern Dengjel and Vyacheslav Akimov and Olsen, {Jesper V} and Jakob Bunkenborg and Matthias Mann and Blagoy Blagoev and Andersen, {Jens S}",
year = "2007",
month = may,
day = "1",
doi = "10.1038/nbt1301",
language = "English",
volume = "25",
pages = "566--568",
journal = "Nature Biotechnology",
issn = "1087-0156",
publisher = "Nature Publishing Group",
number = "5",
}