Quantitative analysis of aroma compounds in carrot (Daucus carota L.) cultivars by capillary gas chromatography using large-volume injection technique

Frank Kjeldsen, Lars P. Christensen*, Merete Edelenbos

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Abstrakt

Dynamic headspace sampling was used to collect aroma compounds from raw samples of four carrot (Daucus carota L.) cultivars (Brasilia, Duke, Fancy, and Cortez). The collected volatiles were analyzed by capillary GC - FID and GC-MS using large-volume cool on-column injection (LVI - COC). Of the 36 compounds identified, 6 had not been previously detected in carrots. Significant differences between the carrot cultivars were found for 31 of the identified volatiles as well as for total monoterpenes, sesquiterpenes, and total volatile content. Mono- and sesquiterpenes accounted for about 98% of the total volatile mass in all cultivars. LVI - COC injection was used to determine the loss of carrot volatiles during concentration of headspace samples under a stream of nitrogen. The loss among major monoterpenes in the concentrated samples varied from 16% for p-cymene to > 40% for α-pinene as compared to nonconcentrated samples. The loss among high-boiling sesquiterpenes varied from not detectable (β-caryophyllene, α-humulene, and caryophyllene oxide) to approximately 7% for (E)- and (Z)-γ-bisabolene.

OriginalsprogEngelsk
Artikelnummerjf010213n
TidsskriftJournal of Agricultural and Food Chemistry
Vol/bind49
Udgave nummer9
Sider (fra-til)4342-4348
Antal sider7
ISSN0021-8561
DOI
StatusUdgivet - 2001
Udgivet eksterntJa

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