Quantification of liver fibrosis at T1 and T2 mapping with extracellular volume fraction MRI: Preclinical results

Julian A. Luetkens, Sabine Klein, Frank Träber, Frederic C. Schmeel, Alois M. Sprinkart, Daniel L.R. Kuetting, Wolfgang Block, Frank E. Uschner, Robert Schierwagen, Kanishka Hittatiya, Glen Kristiansen, Juergen Gieseke, Hans H. Schild, Jonel Trebicka, Guido M. Kukuk*

*Kontaktforfatter for dette arbejde

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Purpose To evaluate MRI T1 and T2 mapping with calculation of extracellular volume (ECV) for diagnosis and grading of liver fibrosis. Materials and Methods Different grades of fibrosis were induced in 60 male Sprague-Dawley rats by bile duct ligation (BDL) and carbon-tetrachloride (CCl 4) intoxication. Portal pressure was measured invasively, whereas hepatic fibrosis was quantified by hydroxyproline content, Sirius red staining, and α smooth muscle actin staining. T1 values, T2 values, and ECV were assessed by using quantitative MRI mapping techniques. Results T1 values in animals 4 weeks after BDL were greater than in control animals (718 msec ± 74 vs 578 msec ± 33, respectively; P < .001). T2 values at 4 weeks were also greater in animals that underwent BDL than in control animals (46 msec ± 6 vs 29 msec ± 2, respectively; P < .001). Similar T1 and T2 findings were observed after CCl 4 intoxication. ECV was greater in animals 4 weeks after BDL compared with control animals (31.3% ± 1.3 vs 18.2% ± 3.5, respectively; P < .001), with similar results after CCl 4 intoxication. High correlations were found between ECV and hepatic hydroxyproline content (BDL: r = 0.68, P < .001; CCl 4: r = 0.65, P < .001), Sirius red staining (BDL: r = 0.88, P < .001; CCl 4: r = 0.82, P < .001), α smooth muscle actin staining (BDL: r = 0.70, P < .001; CCl 4: r = 0.73, P < .001), and portal pressure (BDL: r = 0.54, P = .003; CCl 4: r = 0.39, P = .043). Conclusion Elevation of T1 and T2 values and ECV was associated with severity of liver fibrosis and portal hypertension in an experimental animal model.

OriginalsprogEngelsk
TidsskriftRadiology
Vol/bind288
Udgave nummer3
Sider (fra-til)748-754
ISSN0033-8419
DOI
StatusUdgivet - 1. sep. 2018

Fingeraftryk

Liver Cirrhosis
Ligation
Portal Pressure
Hydroxyproline
Smooth Muscle
Actins
Liver
Portal Hypertension
Sprague Dawley Rats

Citer dette

Luetkens, J. A., Klein, S., Träber, F., Schmeel, F. C., Sprinkart, A. M., Kuetting, D. L. R., ... Kukuk, G. M. (2018). Quantification of liver fibrosis at T1 and T2 mapping with extracellular volume fraction MRI: Preclinical results. Radiology, 288(3), 748-754. https://doi.org/10.1148/radiol.2018180051
Luetkens, Julian A. ; Klein, Sabine ; Träber, Frank ; Schmeel, Frederic C. ; Sprinkart, Alois M. ; Kuetting, Daniel L.R. ; Block, Wolfgang ; Uschner, Frank E. ; Schierwagen, Robert ; Hittatiya, Kanishka ; Kristiansen, Glen ; Gieseke, Juergen ; Schild, Hans H. ; Trebicka, Jonel ; Kukuk, Guido M. / Quantification of liver fibrosis at T1 and T2 mapping with extracellular volume fraction MRI : Preclinical results. I: Radiology. 2018 ; Bind 288, Nr. 3. s. 748-754.
@article{31bfffcfc2644963a5ff4f2be02665cb,
title = "Quantification of liver fibrosis at T1 and T2 mapping with extracellular volume fraction MRI: Preclinical results",
abstract = "Purpose To evaluate MRI T1 and T2 mapping with calculation of extracellular volume (ECV) for diagnosis and grading of liver fibrosis. Materials and Methods Different grades of fibrosis were induced in 60 male Sprague-Dawley rats by bile duct ligation (BDL) and carbon-tetrachloride (CCl 4) intoxication. Portal pressure was measured invasively, whereas hepatic fibrosis was quantified by hydroxyproline content, Sirius red staining, and α smooth muscle actin staining. T1 values, T2 values, and ECV were assessed by using quantitative MRI mapping techniques. Results T1 values in animals 4 weeks after BDL were greater than in control animals (718 msec ± 74 vs 578 msec ± 33, respectively; P < .001). T2 values at 4 weeks were also greater in animals that underwent BDL than in control animals (46 msec ± 6 vs 29 msec ± 2, respectively; P < .001). Similar T1 and T2 findings were observed after CCl 4 intoxication. ECV was greater in animals 4 weeks after BDL compared with control animals (31.3{\%} ± 1.3 vs 18.2{\%} ± 3.5, respectively; P < .001), with similar results after CCl 4 intoxication. High correlations were found between ECV and hepatic hydroxyproline content (BDL: r = 0.68, P < .001; CCl 4: r = 0.65, P < .001), Sirius red staining (BDL: r = 0.88, P < .001; CCl 4: r = 0.82, P < .001), α smooth muscle actin staining (BDL: r = 0.70, P < .001; CCl 4: r = 0.73, P < .001), and portal pressure (BDL: r = 0.54, P = .003; CCl 4: r = 0.39, P = .043). Conclusion Elevation of T1 and T2 values and ECV was associated with severity of liver fibrosis and portal hypertension in an experimental animal model.",
keywords = "Animals, Disease Models, Animal, Image Processing, Computer-Assisted/methods, Liver Cirrhosis/diagnostic imaging, Liver/diagnostic imaging, Magnetic Resonance Imaging/methods, Male, Rats, Rats, Sprague-Dawley, Severity of Illness Index",
author = "Luetkens, {Julian A.} and Sabine Klein and Frank Tr{\"a}ber and Schmeel, {Frederic C.} and Sprinkart, {Alois M.} and Kuetting, {Daniel L.R.} and Wolfgang Block and Uschner, {Frank E.} and Robert Schierwagen and Kanishka Hittatiya and Glen Kristiansen and Juergen Gieseke and Schild, {Hans H.} and Jonel Trebicka and Kukuk, {Guido M.}",
year = "2018",
month = "9",
day = "1",
doi = "10.1148/radiol.2018180051",
language = "English",
volume = "288",
pages = "748--754",
journal = "Radiology",
issn = "0033-8419",
publisher = "Radiological Society of North America, Inc.",
number = "3",

}

Luetkens, JA, Klein, S, Träber, F, Schmeel, FC, Sprinkart, AM, Kuetting, DLR, Block, W, Uschner, FE, Schierwagen, R, Hittatiya, K, Kristiansen, G, Gieseke, J, Schild, HH, Trebicka, J & Kukuk, GM 2018, 'Quantification of liver fibrosis at T1 and T2 mapping with extracellular volume fraction MRI: Preclinical results', Radiology, bind 288, nr. 3, s. 748-754. https://doi.org/10.1148/radiol.2018180051

Quantification of liver fibrosis at T1 and T2 mapping with extracellular volume fraction MRI : Preclinical results. / Luetkens, Julian A.; Klein, Sabine; Träber, Frank; Schmeel, Frederic C.; Sprinkart, Alois M.; Kuetting, Daniel L.R.; Block, Wolfgang; Uschner, Frank E.; Schierwagen, Robert; Hittatiya, Kanishka; Kristiansen, Glen; Gieseke, Juergen; Schild, Hans H.; Trebicka, Jonel; Kukuk, Guido M.

I: Radiology, Bind 288, Nr. 3, 01.09.2018, s. 748-754.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Quantification of liver fibrosis at T1 and T2 mapping with extracellular volume fraction MRI

T2 - Preclinical results

AU - Luetkens, Julian A.

AU - Klein, Sabine

AU - Träber, Frank

AU - Schmeel, Frederic C.

AU - Sprinkart, Alois M.

AU - Kuetting, Daniel L.R.

AU - Block, Wolfgang

AU - Uschner, Frank E.

AU - Schierwagen, Robert

AU - Hittatiya, Kanishka

AU - Kristiansen, Glen

AU - Gieseke, Juergen

AU - Schild, Hans H.

AU - Trebicka, Jonel

AU - Kukuk, Guido M.

PY - 2018/9/1

Y1 - 2018/9/1

N2 - Purpose To evaluate MRI T1 and T2 mapping with calculation of extracellular volume (ECV) for diagnosis and grading of liver fibrosis. Materials and Methods Different grades of fibrosis were induced in 60 male Sprague-Dawley rats by bile duct ligation (BDL) and carbon-tetrachloride (CCl 4) intoxication. Portal pressure was measured invasively, whereas hepatic fibrosis was quantified by hydroxyproline content, Sirius red staining, and α smooth muscle actin staining. T1 values, T2 values, and ECV were assessed by using quantitative MRI mapping techniques. Results T1 values in animals 4 weeks after BDL were greater than in control animals (718 msec ± 74 vs 578 msec ± 33, respectively; P < .001). T2 values at 4 weeks were also greater in animals that underwent BDL than in control animals (46 msec ± 6 vs 29 msec ± 2, respectively; P < .001). Similar T1 and T2 findings were observed after CCl 4 intoxication. ECV was greater in animals 4 weeks after BDL compared with control animals (31.3% ± 1.3 vs 18.2% ± 3.5, respectively; P < .001), with similar results after CCl 4 intoxication. High correlations were found between ECV and hepatic hydroxyproline content (BDL: r = 0.68, P < .001; CCl 4: r = 0.65, P < .001), Sirius red staining (BDL: r = 0.88, P < .001; CCl 4: r = 0.82, P < .001), α smooth muscle actin staining (BDL: r = 0.70, P < .001; CCl 4: r = 0.73, P < .001), and portal pressure (BDL: r = 0.54, P = .003; CCl 4: r = 0.39, P = .043). Conclusion Elevation of T1 and T2 values and ECV was associated with severity of liver fibrosis and portal hypertension in an experimental animal model.

AB - Purpose To evaluate MRI T1 and T2 mapping with calculation of extracellular volume (ECV) for diagnosis and grading of liver fibrosis. Materials and Methods Different grades of fibrosis were induced in 60 male Sprague-Dawley rats by bile duct ligation (BDL) and carbon-tetrachloride (CCl 4) intoxication. Portal pressure was measured invasively, whereas hepatic fibrosis was quantified by hydroxyproline content, Sirius red staining, and α smooth muscle actin staining. T1 values, T2 values, and ECV were assessed by using quantitative MRI mapping techniques. Results T1 values in animals 4 weeks after BDL were greater than in control animals (718 msec ± 74 vs 578 msec ± 33, respectively; P < .001). T2 values at 4 weeks were also greater in animals that underwent BDL than in control animals (46 msec ± 6 vs 29 msec ± 2, respectively; P < .001). Similar T1 and T2 findings were observed after CCl 4 intoxication. ECV was greater in animals 4 weeks after BDL compared with control animals (31.3% ± 1.3 vs 18.2% ± 3.5, respectively; P < .001), with similar results after CCl 4 intoxication. High correlations were found between ECV and hepatic hydroxyproline content (BDL: r = 0.68, P < .001; CCl 4: r = 0.65, P < .001), Sirius red staining (BDL: r = 0.88, P < .001; CCl 4: r = 0.82, P < .001), α smooth muscle actin staining (BDL: r = 0.70, P < .001; CCl 4: r = 0.73, P < .001), and portal pressure (BDL: r = 0.54, P = .003; CCl 4: r = 0.39, P = .043). Conclusion Elevation of T1 and T2 values and ECV was associated with severity of liver fibrosis and portal hypertension in an experimental animal model.

KW - Animals

KW - Disease Models, Animal

KW - Image Processing, Computer-Assisted/methods

KW - Liver Cirrhosis/diagnostic imaging

KW - Liver/diagnostic imaging

KW - Magnetic Resonance Imaging/methods

KW - Male

KW - Rats

KW - Rats, Sprague-Dawley

KW - Severity of Illness Index

U2 - 10.1148/radiol.2018180051

DO - 10.1148/radiol.2018180051

M3 - Journal article

C2 - 29944086

AN - SCOPUS:85052655949

VL - 288

SP - 748

EP - 754

JO - Radiology

JF - Radiology

SN - 0033-8419

IS - 3

ER -

Luetkens JA, Klein S, Träber F, Schmeel FC, Sprinkart AM, Kuetting DLR et al. Quantification of liver fibrosis at T1 and T2 mapping with extracellular volume fraction MRI: Preclinical results. Radiology. 2018 sep 1;288(3):748-754. https://doi.org/10.1148/radiol.2018180051