Pyrene-perylene as a FRET pair coupled to the N2'-functionality of 2'-amino-LNA

Dorthe Lindegaard; Andreas Stahl Madsen; Irina V. Astakhova; Andrei D. Malakhov; Bolle Ravindra Babu; Vladimir A. Korshun; Jesper Wengel

doi:10.1016/j.bmc.2007.04.056

Pyrene-perylene as a FRET pair coupled to the N2'-functionality of 2'-amino-LNA

Dorthe Lindegaard, Andreas Stahl Madsen, Irina V. Astakhova, Andrei D. Malakhov, Bolle Ravindra Babu, Vladimir A. Korshun, Jesper Wengel

Institut for Fysik, Kemi og Farmaci

Russian Academy of Sciences

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

Abstract

Detection of nucleic acid hybridization via fluorescence resonance energy transfer (FRET) using pyren-1-ylmethyl and perylen-3-ylmethyl N2′-functionalized 2′-amino-LNA nucleosides incorporated into oligonucleotides exhibited a clear distance dependence of the FRET efficiency, ranging from below 10% when the fluorophores were approximately 40 Å apart to approximately 90% when the fluorophores were in close proximity.

Originalsprog	Engelsk
Tidsskrift	Bioorganic & Medicinal Chemistry
Vol/bind	16
Udgave nummer	1
Sider (fra-til)	94-99
Antal sider	6
ISSN	0968-0896
DOI	https://doi.org/10.1016/j.bmc.2007.04.056
Status	Udgivet - 2008

Dokumenter og links

10.1016/j.bmc.2007.04.056

Citationsformater

@article{20af3660d36a11dcb2ba000ea68e967b,

title = "Pyrene-perylene as a FRET pair coupled to the N2'-functionality of 2'-amino-LNA",

abstract = "Detection of nucleic acid hybridization via fluorescence resonance energy transfer (FRET) using pyren-1-ylmethyl and perylen-3-ylmethyl N2′-functionalized 2′-amino-LNA nucleosides incorporated into oligonucleotides exhibited a clear distance dependence of the FRET efficiency, ranging from below 10% when the fluorophores were approximately 40 {\AA} apart to approximately 90% when the fluorophores were in close proximity.",

keywords = "Amino-LNA, Pyrene, Perylene, FRET",

author = "Dorthe Lindegaard and Madsen, {Andreas Stahl} and Astakhova, {Irina V.} and Malakhov, {Andrei D.} and Babu, {Bolle Ravindra} and Korshun, {Vladimir A.} and Jesper Wengel",

year = "2008",

doi = "10.1016/j.bmc.2007.04.056",

language = "English",

volume = "16",

pages = "94--99",

journal = "Bioorganic & Medicinal Chemistry",

issn = "0968-0896",

publisher = "Elsevier",

number = "1",

}

TY - JOUR

T1 - Pyrene-perylene as a FRET pair coupled to the N2'-functionality of 2'-amino-LNA

AU - Lindegaard, Dorthe

AU - Madsen, Andreas Stahl

AU - Astakhova, Irina V.

AU - Malakhov, Andrei D.

AU - Babu, Bolle Ravindra

AU - Korshun, Vladimir A.

AU - Wengel, Jesper

PY - 2008

Y1 - 2008

N2 - Detection of nucleic acid hybridization via fluorescence resonance energy transfer (FRET) using pyren-1-ylmethyl and perylen-3-ylmethyl N2′-functionalized 2′-amino-LNA nucleosides incorporated into oligonucleotides exhibited a clear distance dependence of the FRET efficiency, ranging from below 10% when the fluorophores were approximately 40 Å apart to approximately 90% when the fluorophores were in close proximity.

AB - Detection of nucleic acid hybridization via fluorescence resonance energy transfer (FRET) using pyren-1-ylmethyl and perylen-3-ylmethyl N2′-functionalized 2′-amino-LNA nucleosides incorporated into oligonucleotides exhibited a clear distance dependence of the FRET efficiency, ranging from below 10% when the fluorophores were approximately 40 Å apart to approximately 90% when the fluorophores were in close proximity.

KW - Amino-LNA

KW - Pyrene

KW - Perylene

KW - FRET

U2 - 10.1016/j.bmc.2007.04.056

DO - 10.1016/j.bmc.2007.04.056

M3 - Journal article

SN - 0968-0896

VL - 16

SP - 94

EP - 99

JO - Bioorganic & Medicinal Chemistry

JF - Bioorganic & Medicinal Chemistry

IS - 1

ER -

Pyrene-perylene as a FRET pair coupled to the N2'-functionality of 2'-amino-LNA

Abstract

Dokumenter og links

Fingeraftryk

Citationsformater