Purification of GFP-tagged nuclei from frozen livers of INTACT mice for RNA- and ATAC-sequencing

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Abstract

Isolation of nuclei tagged in specific cell types (INTACT) allows for stress-free and high-throughput analyses of cellular subpopulations. Here, we present an improved protocol for isolation of pure and high-quality GFP-labeled nuclei from frozen livers of INTACT mice, as well as protocols for downstream sequencing analyses. The adaptation to frozen tissue provides a pause point that allows sampling at multiple time points and/or phenotypic characterization of livers prior to nuclei isolation and downstream analyses. For complete details on the use of this protocol, please refer to Loft et al. (2021).

OriginalsprogEngelsk
TidsskriftSTAR Protocols
Vol/bind2
Udgave nummer3
Sider (fra-til)100805
ISSN2666-1667
DOI
StatusUdgivet - 17. sep. 2021

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© 2021 The Author(s).

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