Proteomic profiling of pretreatment serum from HIV-infected patients identifies candidate markers predictive of lymphoma development

Maja Ølholm Vase*, Maja Ludvigsen, Knud Bendix, Stephen Hamilton-Dutoit, Michael Boe Moller, Court Pedersen, Gitte Pedersen, Niels Obel, Carsten Schade Larsen, Francesco D'Amore, Bent Honoré

*Kontaktforfatter for dette arbejde

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Objective: HIV-infected individuals have an increased risk of developing lymphoma. We sought to identify markers predictive of lymphoma development by comparing protein expression patterns in serum obtained at the time of HIV diagnosis from patients who later developed malignant lymphoma or benign lymphadenopathy, with samples from patients with no subsequent history of neoplasia. Design: All patients were identified retrospectively from the Danish HIV cohort. Methods: Serum samples (N=21), obtained at time of HIV diagnosis, were subjected to high-resolution two-dimensional gel electrophoresis. Differentially expressed proteins were identified by liquid chromatography-tandem mass spectrometry. A tissue microarray, containing diagnostic HIV-lymphoma tissue samples (N=40), was used to investigate immunohistochemical expression of markers in tumoural lesions. Results: Fourteen differentially expressed protein spots were detected. Using principal components analysis, spots containing immunoglobulin J chain, apolipoprotein A-I, procollagen C-endopeptidase enhancer-1 and complement C4-A were associated with lymphoma development (P

OriginalsprogEngelsk
TidsskriftAIDS
Vol/bind30
Udgave nummer12
Sider (fra-til)1889-1898
ISSN0269-9370
DOI
StatusUdgivet - 2016

Fingeraftryk

Lymphoma
HIV
Serum
Immunoglobulin J-Chains
Proteins
Apolipoprotein A-I
Tandem Mass Spectrometry
Liquid Chromatography
Neoplasms

Citer dette

Vase, Maja Ølholm ; Ludvigsen, Maja ; Bendix, Knud ; Hamilton-Dutoit, Stephen ; Moller, Michael Boe ; Pedersen, Court ; Pedersen, Gitte ; Obel, Niels ; Larsen, Carsten Schade ; D'Amore, Francesco ; Honoré, Bent. / Proteomic profiling of pretreatment serum from HIV-infected patients identifies candidate markers predictive of lymphoma development. I: AIDS. 2016 ; Bind 30, Nr. 12. s. 1889-1898.
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title = "Proteomic profiling of pretreatment serum from HIV-infected patients identifies candidate markers predictive of lymphoma development",
abstract = "Objective: HIV-infected individuals have an increased risk of developing lymphoma. We sought to identify markers predictive of lymphoma development by comparing protein expression patterns in serum obtained at the time of HIV diagnosis from patients who later developed malignant lymphoma or benign lymphadenopathy, with samples from patients with no subsequent history of neoplasia. Design: All patients were identified retrospectively from the Danish HIV cohort. Methods: Serum samples (N=21), obtained at time of HIV diagnosis, were subjected to high-resolution two-dimensional gel electrophoresis. Differentially expressed proteins were identified by liquid chromatography-tandem mass spectrometry. A tissue microarray, containing diagnostic HIV-lymphoma tissue samples (N=40), was used to investigate immunohistochemical expression of markers in tumoural lesions. Results: Fourteen differentially expressed protein spots were detected. Using principal components analysis, spots containing immunoglobulin J chain, apolipoprotein A-I, procollagen C-endopeptidase enhancer-1 and complement C4-A were associated with lymphoma development (P",
keywords = "biological markers, haematology, lymphadenopathy/lymphoid tissue, lymphoma, proteomics",
author = "Vase, {Maja {\O}lholm} and Maja Ludvigsen and Knud Bendix and Stephen Hamilton-Dutoit and Moller, {Michael Boe} and Court Pedersen and Gitte Pedersen and Niels Obel and Larsen, {Carsten Schade} and Francesco D'Amore and Bent Honor{\'e}",
year = "2016",
doi = "10.1097/QAD.0000000000001152",
language = "English",
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Vase, MØ, Ludvigsen, M, Bendix, K, Hamilton-Dutoit, S, Moller, MB, Pedersen, C, Pedersen, G, Obel, N, Larsen, CS, D'Amore, F & Honoré, B 2016, 'Proteomic profiling of pretreatment serum from HIV-infected patients identifies candidate markers predictive of lymphoma development', AIDS, bind 30, nr. 12, s. 1889-1898. https://doi.org/10.1097/QAD.0000000000001152

Proteomic profiling of pretreatment serum from HIV-infected patients identifies candidate markers predictive of lymphoma development. / Vase, Maja Ølholm; Ludvigsen, Maja; Bendix, Knud; Hamilton-Dutoit, Stephen; Moller, Michael Boe; Pedersen, Court; Pedersen, Gitte; Obel, Niels; Larsen, Carsten Schade; D'Amore, Francesco; Honoré, Bent.

I: AIDS, Bind 30, Nr. 12, 2016, s. 1889-1898.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Proteomic profiling of pretreatment serum from HIV-infected patients identifies candidate markers predictive of lymphoma development

AU - Vase, Maja Ølholm

AU - Ludvigsen, Maja

AU - Bendix, Knud

AU - Hamilton-Dutoit, Stephen

AU - Moller, Michael Boe

AU - Pedersen, Court

AU - Pedersen, Gitte

AU - Obel, Niels

AU - Larsen, Carsten Schade

AU - D'Amore, Francesco

AU - Honoré, Bent

PY - 2016

Y1 - 2016

N2 - Objective: HIV-infected individuals have an increased risk of developing lymphoma. We sought to identify markers predictive of lymphoma development by comparing protein expression patterns in serum obtained at the time of HIV diagnosis from patients who later developed malignant lymphoma or benign lymphadenopathy, with samples from patients with no subsequent history of neoplasia. Design: All patients were identified retrospectively from the Danish HIV cohort. Methods: Serum samples (N=21), obtained at time of HIV diagnosis, were subjected to high-resolution two-dimensional gel electrophoresis. Differentially expressed proteins were identified by liquid chromatography-tandem mass spectrometry. A tissue microarray, containing diagnostic HIV-lymphoma tissue samples (N=40), was used to investigate immunohistochemical expression of markers in tumoural lesions. Results: Fourteen differentially expressed protein spots were detected. Using principal components analysis, spots containing immunoglobulin J chain, apolipoprotein A-I, procollagen C-endopeptidase enhancer-1 and complement C4-A were associated with lymphoma development (P

AB - Objective: HIV-infected individuals have an increased risk of developing lymphoma. We sought to identify markers predictive of lymphoma development by comparing protein expression patterns in serum obtained at the time of HIV diagnosis from patients who later developed malignant lymphoma or benign lymphadenopathy, with samples from patients with no subsequent history of neoplasia. Design: All patients were identified retrospectively from the Danish HIV cohort. Methods: Serum samples (N=21), obtained at time of HIV diagnosis, were subjected to high-resolution two-dimensional gel electrophoresis. Differentially expressed proteins were identified by liquid chromatography-tandem mass spectrometry. A tissue microarray, containing diagnostic HIV-lymphoma tissue samples (N=40), was used to investigate immunohistochemical expression of markers in tumoural lesions. Results: Fourteen differentially expressed protein spots were detected. Using principal components analysis, spots containing immunoglobulin J chain, apolipoprotein A-I, procollagen C-endopeptidase enhancer-1 and complement C4-A were associated with lymphoma development (P

KW - biological markers

KW - haematology

KW - lymphadenopathy/lymphoid tissue

KW - lymphoma

KW - proteomics

U2 - 10.1097/QAD.0000000000001152

DO - 10.1097/QAD.0000000000001152

M3 - Journal article

C2 - 27177314

AN - SCOPUS:84966747137

VL - 30

SP - 1889

EP - 1898

JO - AIDS

JF - AIDS

SN - 0269-9370

IS - 12

ER -