Proteomic profiling of Plasmodium sporozoite maturation identifies new proteins essential for parasite development and infectivity

Edwin Lasonder, Chris J Janse, Geert-Jan van Gemert, Gunnar R Mair, Adriaan M W Vermunt, Bruno G Douradinha, Vera van Noort, Martijn A Huynen, Adrian J F Luty, Hans Kroeze, Shahid M Khan, Robert W Sauerwein, Andrew P Waters, Matthias Mann, Hendrik G Stunnenberg

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Udgivelsesdato: 2008-Oct
OriginalsprogEngelsk
TidsskriftPLoS Pathogens
Vol/bind4
Udgave nummer10
Sider (fra-til)e1000195
ISSN1553-7366
DOI
StatusUdgivet - 1. okt. 2008

Fingeraftryk

Sporozoites
Parasites
Proteins
Proteome
Malaria
Plasmodium falciparum
Hepatocytes
Rodentia

Citer dette

Lasonder, E., Janse, C. J., van Gemert, G-J., Mair, G. R., Vermunt, A. M. W., Douradinha, B. G., ... Stunnenberg, H. G. (2008). Proteomic profiling of Plasmodium sporozoite maturation identifies new proteins essential for parasite development and infectivity. PLoS Pathogens, 4(10), e1000195. https://doi.org/10.1371/journal.ppat.1000195
Lasonder, Edwin ; Janse, Chris J ; van Gemert, Geert-Jan ; Mair, Gunnar R ; Vermunt, Adriaan M W ; Douradinha, Bruno G ; van Noort, Vera ; Huynen, Martijn A ; Luty, Adrian J F ; Kroeze, Hans ; Khan, Shahid M ; Sauerwein, Robert W ; Waters, Andrew P ; Mann, Matthias ; Stunnenberg, Hendrik G. / Proteomic profiling of Plasmodium sporozoite maturation identifies new proteins essential for parasite development and infectivity. I: PLoS Pathogens. 2008 ; Bind 4, Nr. 10. s. e1000195.
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title = "Proteomic profiling of Plasmodium sporozoite maturation identifies new proteins essential for parasite development and infectivity",
abstract = "Plasmodium falciparum sporozoites that develop and mature inside an Anopheles mosquito initiate a malaria infection in humans. Here we report the first proteomic comparison of different parasite stages from the mosquito -- early and late oocysts containing midgut sporozoites, and the mature, infectious salivary gland sporozoites. Despite the morphological similarity between midgut and salivary gland sporozoites, their proteomes are markedly different, in agreement with their increase in hepatocyte infectivity. The different sporozoite proteomes contain a large number of stage specific proteins whose annotation suggest an involvement in sporozoite maturation, motility, infection of the human host and associated metabolic adjustments. Analyses of proteins identified in the P. falciparum sporozoite proteomes by orthologous gene disruption in the rodent malaria parasite, P. berghei, revealed three previously uncharacterized Plasmodium proteins that appear to be essential for sporozoite development at distinct points of maturation in the mosquito. This study sheds light on the development and maturation of the malaria parasite in an Anopheles mosquito and also identifies proteins that may be essential for sporozoite infectivity to humans.",
author = "Edwin Lasonder and Janse, {Chris J} and {van Gemert}, Geert-Jan and Mair, {Gunnar R} and Vermunt, {Adriaan M W} and Douradinha, {Bruno G} and {van Noort}, Vera and Huynen, {Martijn A} and Luty, {Adrian J F} and Hans Kroeze and Khan, {Shahid M} and Sauerwein, {Robert W} and Waters, {Andrew P} and Matthias Mann and Stunnenberg, {Hendrik G}",
year = "2008",
month = "10",
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doi = "10.1371/journal.ppat.1000195",
language = "English",
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Lasonder, E, Janse, CJ, van Gemert, G-J, Mair, GR, Vermunt, AMW, Douradinha, BG, van Noort, V, Huynen, MA, Luty, AJF, Kroeze, H, Khan, SM, Sauerwein, RW, Waters, AP, Mann, M & Stunnenberg, HG 2008, 'Proteomic profiling of Plasmodium sporozoite maturation identifies new proteins essential for parasite development and infectivity', PLoS Pathogens, bind 4, nr. 10, s. e1000195. https://doi.org/10.1371/journal.ppat.1000195

Proteomic profiling of Plasmodium sporozoite maturation identifies new proteins essential for parasite development and infectivity. / Lasonder, Edwin; Janse, Chris J; van Gemert, Geert-Jan; Mair, Gunnar R; Vermunt, Adriaan M W; Douradinha, Bruno G; van Noort, Vera; Huynen, Martijn A; Luty, Adrian J F; Kroeze, Hans; Khan, Shahid M; Sauerwein, Robert W; Waters, Andrew P; Mann, Matthias; Stunnenberg, Hendrik G.

I: PLoS Pathogens, Bind 4, Nr. 10, 01.10.2008, s. e1000195.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Proteomic profiling of Plasmodium sporozoite maturation identifies new proteins essential for parasite development and infectivity

AU - Lasonder, Edwin

AU - Janse, Chris J

AU - van Gemert, Geert-Jan

AU - Mair, Gunnar R

AU - Vermunt, Adriaan M W

AU - Douradinha, Bruno G

AU - van Noort, Vera

AU - Huynen, Martijn A

AU - Luty, Adrian J F

AU - Kroeze, Hans

AU - Khan, Shahid M

AU - Sauerwein, Robert W

AU - Waters, Andrew P

AU - Mann, Matthias

AU - Stunnenberg, Hendrik G

PY - 2008/10/1

Y1 - 2008/10/1

N2 - Plasmodium falciparum sporozoites that develop and mature inside an Anopheles mosquito initiate a malaria infection in humans. Here we report the first proteomic comparison of different parasite stages from the mosquito -- early and late oocysts containing midgut sporozoites, and the mature, infectious salivary gland sporozoites. Despite the morphological similarity between midgut and salivary gland sporozoites, their proteomes are markedly different, in agreement with their increase in hepatocyte infectivity. The different sporozoite proteomes contain a large number of stage specific proteins whose annotation suggest an involvement in sporozoite maturation, motility, infection of the human host and associated metabolic adjustments. Analyses of proteins identified in the P. falciparum sporozoite proteomes by orthologous gene disruption in the rodent malaria parasite, P. berghei, revealed three previously uncharacterized Plasmodium proteins that appear to be essential for sporozoite development at distinct points of maturation in the mosquito. This study sheds light on the development and maturation of the malaria parasite in an Anopheles mosquito and also identifies proteins that may be essential for sporozoite infectivity to humans.

AB - Plasmodium falciparum sporozoites that develop and mature inside an Anopheles mosquito initiate a malaria infection in humans. Here we report the first proteomic comparison of different parasite stages from the mosquito -- early and late oocysts containing midgut sporozoites, and the mature, infectious salivary gland sporozoites. Despite the morphological similarity between midgut and salivary gland sporozoites, their proteomes are markedly different, in agreement with their increase in hepatocyte infectivity. The different sporozoite proteomes contain a large number of stage specific proteins whose annotation suggest an involvement in sporozoite maturation, motility, infection of the human host and associated metabolic adjustments. Analyses of proteins identified in the P. falciparum sporozoite proteomes by orthologous gene disruption in the rodent malaria parasite, P. berghei, revealed three previously uncharacterized Plasmodium proteins that appear to be essential for sporozoite development at distinct points of maturation in the mosquito. This study sheds light on the development and maturation of the malaria parasite in an Anopheles mosquito and also identifies proteins that may be essential for sporozoite infectivity to humans.

U2 - 10.1371/journal.ppat.1000195

DO - 10.1371/journal.ppat.1000195

M3 - Journal article

VL - 4

SP - e1000195

JO - P L o S Pathogens

JF - P L o S Pathogens

SN - 1553-7366

IS - 10

ER -