Proteomic analysis of the Arabidopsis nucleolus suggests novel nucleolar functions

Alison F Pendle, Gillian P Clark, Reinier Boon, Dominika Lewandowska, Yun Wah Lam, Jens Andersen, Matthias Mann, Angus I Lamond, John W S Brown, Peter J Shaw

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Udgivelsesdato: 2005-Jan
OriginalsprogEngelsk
TidsskriftMolecular Biology of the Cell
Vol/bind16
Udgave nummer1
Sider (fra-til)260-9
ISSN1059-1524
DOI
StatusUdgivet - 2005

Fingeraftryk

Arabidopsis
Proteome
Proteins
Nonsense Mediated mRNA Decay
Plant Proteins
Messenger RNA
Nuclear Proteins
RNA

Citer dette

Pendle, A. F., Clark, G. P., Boon, R., Lewandowska, D., Lam, Y. W., Andersen, J., ... Shaw, P. J. (2005). Proteomic analysis of the Arabidopsis nucleolus suggests novel nucleolar functions. Molecular Biology of the Cell, 16(1), 260-9. https://doi.org/10.1091/mbc.E04-09-0791
Pendle, Alison F ; Clark, Gillian P ; Boon, Reinier ; Lewandowska, Dominika ; Lam, Yun Wah ; Andersen, Jens ; Mann, Matthias ; Lamond, Angus I ; Brown, John W S ; Shaw, Peter J. / Proteomic analysis of the Arabidopsis nucleolus suggests novel nucleolar functions. I: Molecular Biology of the Cell. 2005 ; Bind 16, Nr. 1. s. 260-9.
@article{c0592cb0e10411db9628000ea68e967b,
title = "Proteomic analysis of the Arabidopsis nucleolus suggests novel nucleolar functions",
abstract = "The eukaryotic nucleolus is involved in ribosome biogenesis and a wide range of other RNA metabolism and cellular functions. An important step in the functional analysis of the nucleolus is to determine the complement of proteins of this nuclear compartment. Here, we describe the first proteomic analysis of plant (Arabidopsis thaliana) nucleoli, in which we have identified 217 proteins. This allows a direct comparison of the proteomes of an important nuclear structure between two widely divergent species: human and Arabidopsis. The comparison identified many common proteins, plant-specific proteins, proteins of unknown function found in both proteomes, and proteins that were nucleolar in plants but nonnucleolar in human. Seventy-two proteins were expressed as GFP fusions and 87{\%} showed nucleolar or nucleolar-associated localization. In a striking and unexpected finding, we have identified six components of the postsplicing exon-junction complex (EJC) involved in mRNA export and nonsense-mediated decay (NMD)/mRNA surveillance. This association was confirmed by GFP-fusion protein localization. These results raise the possibility that in plants, nucleoli may have additional functions in mRNA export or surveillance.",
keywords = "Arabidopsis, Arabidopsis Proteins, Cell Nucleolus, Cell Nucleus, Exons, Gene Expression Regulation, Plant, Genes, Plant, Green Fluorescent Proteins, Image Processing, Computer-Assisted, Mass Spectrometry, Microscopy, Fluorescence, Proteomics, RNA, RNA, Messenger, Recombinant Fusion Proteins, Sequence Analysis, DNA",
author = "Pendle, {Alison F} and Clark, {Gillian P} and Reinier Boon and Dominika Lewandowska and Lam, {Yun Wah} and Jens Andersen and Matthias Mann and Lamond, {Angus I} and Brown, {John W S} and Shaw, {Peter J}",
year = "2005",
doi = "10.1091/mbc.E04-09-0791",
language = "English",
volume = "16",
pages = "260--9",
journal = "Molecular Biology of the Cell",
issn = "1059-1524",
publisher = "American Society for Cell Biology",
number = "1",

}

Pendle, AF, Clark, GP, Boon, R, Lewandowska, D, Lam, YW, Andersen, J, Mann, M, Lamond, AI, Brown, JWS & Shaw, PJ 2005, 'Proteomic analysis of the Arabidopsis nucleolus suggests novel nucleolar functions', Molecular Biology of the Cell, bind 16, nr. 1, s. 260-9. https://doi.org/10.1091/mbc.E04-09-0791

Proteomic analysis of the Arabidopsis nucleolus suggests novel nucleolar functions. / Pendle, Alison F; Clark, Gillian P; Boon, Reinier; Lewandowska, Dominika; Lam, Yun Wah; Andersen, Jens; Mann, Matthias; Lamond, Angus I; Brown, John W S; Shaw, Peter J.

I: Molecular Biology of the Cell, Bind 16, Nr. 1, 2005, s. 260-9.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Proteomic analysis of the Arabidopsis nucleolus suggests novel nucleolar functions

AU - Pendle, Alison F

AU - Clark, Gillian P

AU - Boon, Reinier

AU - Lewandowska, Dominika

AU - Lam, Yun Wah

AU - Andersen, Jens

AU - Mann, Matthias

AU - Lamond, Angus I

AU - Brown, John W S

AU - Shaw, Peter J

PY - 2005

Y1 - 2005

N2 - The eukaryotic nucleolus is involved in ribosome biogenesis and a wide range of other RNA metabolism and cellular functions. An important step in the functional analysis of the nucleolus is to determine the complement of proteins of this nuclear compartment. Here, we describe the first proteomic analysis of plant (Arabidopsis thaliana) nucleoli, in which we have identified 217 proteins. This allows a direct comparison of the proteomes of an important nuclear structure between two widely divergent species: human and Arabidopsis. The comparison identified many common proteins, plant-specific proteins, proteins of unknown function found in both proteomes, and proteins that were nucleolar in plants but nonnucleolar in human. Seventy-two proteins were expressed as GFP fusions and 87% showed nucleolar or nucleolar-associated localization. In a striking and unexpected finding, we have identified six components of the postsplicing exon-junction complex (EJC) involved in mRNA export and nonsense-mediated decay (NMD)/mRNA surveillance. This association was confirmed by GFP-fusion protein localization. These results raise the possibility that in plants, nucleoli may have additional functions in mRNA export or surveillance.

AB - The eukaryotic nucleolus is involved in ribosome biogenesis and a wide range of other RNA metabolism and cellular functions. An important step in the functional analysis of the nucleolus is to determine the complement of proteins of this nuclear compartment. Here, we describe the first proteomic analysis of plant (Arabidopsis thaliana) nucleoli, in which we have identified 217 proteins. This allows a direct comparison of the proteomes of an important nuclear structure between two widely divergent species: human and Arabidopsis. The comparison identified many common proteins, plant-specific proteins, proteins of unknown function found in both proteomes, and proteins that were nucleolar in plants but nonnucleolar in human. Seventy-two proteins were expressed as GFP fusions and 87% showed nucleolar or nucleolar-associated localization. In a striking and unexpected finding, we have identified six components of the postsplicing exon-junction complex (EJC) involved in mRNA export and nonsense-mediated decay (NMD)/mRNA surveillance. This association was confirmed by GFP-fusion protein localization. These results raise the possibility that in plants, nucleoli may have additional functions in mRNA export or surveillance.

KW - Arabidopsis

KW - Arabidopsis Proteins

KW - Cell Nucleolus

KW - Cell Nucleus

KW - Exons

KW - Gene Expression Regulation, Plant

KW - Genes, Plant

KW - Green Fluorescent Proteins

KW - Image Processing, Computer-Assisted

KW - Mass Spectrometry

KW - Microscopy, Fluorescence

KW - Proteomics

KW - RNA

KW - RNA, Messenger

KW - Recombinant Fusion Proteins

KW - Sequence Analysis, DNA

U2 - 10.1091/mbc.E04-09-0791

DO - 10.1091/mbc.E04-09-0791

M3 - Journal article

C2 - 15496452

VL - 16

SP - 260

EP - 269

JO - Molecular Biology of the Cell

JF - Molecular Biology of the Cell

SN - 1059-1524

IS - 1

ER -