Proteome analysis of interleukin-1beta-induced changes in protein expression in rat islets of Langerhans

P M Larsen, S J Fey, M R Larsen, A Nawrocki, H U Andersen, H Kähler, C Heilmann, M C Voss, P Roepstorff, F Pociot, A E Karlsen, J Nerup

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Udgivelsesdato: 2001-May
OriginalsprogEngelsk
TidsskriftDiabetes
Vol/bind50
Udgave nummer5
Sider (fra-til)1056-63
Antal sider7
ISSN0046-0192
StatusUdgivet - 1. maj 2001

Fingeraftryk

Proteome
Interleukin-1beta
Islets of Langerhans
Proteins
Cell Death
Down-Regulation
Apoptosis

Citer dette

Larsen, P. M., Fey, S. J., Larsen, M. R., Nawrocki, A., Andersen, H. U., Kähler, H., ... Nerup, J. (2001). Proteome analysis of interleukin-1beta-induced changes in protein expression in rat islets of Langerhans. Diabetes, 50(5), 1056-63.
Larsen, P M ; Fey, S J ; Larsen, M R ; Nawrocki, A ; Andersen, H U ; Kähler, H ; Heilmann, C ; Voss, M C ; Roepstorff, P ; Pociot, F ; Karlsen, A E ; Nerup, J. / Proteome analysis of interleukin-1beta-induced changes in protein expression in rat islets of Langerhans. I: Diabetes. 2001 ; Bind 50, Nr. 5. s. 1056-63.
@article{85f793e0651011ddb1a1000ea68e967b,
title = "Proteome analysis of interleukin-1beta-induced changes in protein expression in rat islets of Langerhans",
abstract = "The intracellular molecular events involved in the beta-cell death process are complex but poorly understood. Cytokines, e.g., interleukin (IL)-1beta, may play a crucial role in inducing this process. Protein synthesis is necessary for the deleterious effect of IL-1, and induction of both protective and deleterious proteins has been described. To characterize the rather complex pattern of islet protein expression in rat islets in response to IL-1, we have attempted to identify proteins of altered expression level after IL-1 exposure by 2D gel electrophoresis and mass spectrometry. Of 105 significantly changed (i.e., up- or downregulated or de novo-induced) protein spots, we obtained positive protein identification for 60 protein spots. The 60 identifications corresponded to 57 different proteins. Of these, 10 proteins were present in two to four spots, suggesting that posttranslatory modifications had occurred. In addition, 11 spots contained more than one protein. The proteins could be classified according to their function into the following groups: 1) energy transduction; 2) glycolytic pathway; 3) protein synthesis, chaperones, and protein folding; and 4) signal transduction, regulation, differentiation, and apoptosis. In conclusion, valuable information about the molecular mechanisms involved in cytokine-mediated beta-cell destruction was obtained by this approach.",
keywords = "Animals, Cells, Cultured, Electrophoresis, Gel, Two-Dimensional, Energy Metabolism, Gene Expression Regulation, Interleukin-1, Islets of Langerhans, Mass Spectrometry, Oxidation-Reduction, Proteins, Proteome, Rats",
author = "Larsen, {P M} and Fey, {S J} and Larsen, {M R} and A Nawrocki and Andersen, {H U} and H K{\"a}hler and C Heilmann and Voss, {M C} and P Roepstorff and F Pociot and Karlsen, {A E} and J Nerup",
year = "2001",
month = "5",
day = "1",
language = "English",
volume = "50",
pages = "1056--63",
journal = "Diabetes",
issn = "0012-1797",
publisher = "American Diabetes Association",
number = "5",

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Larsen, PM, Fey, SJ, Larsen, MR, Nawrocki, A, Andersen, HU, Kähler, H, Heilmann, C, Voss, MC, Roepstorff, P, Pociot, F, Karlsen, AE & Nerup, J 2001, 'Proteome analysis of interleukin-1beta-induced changes in protein expression in rat islets of Langerhans', Diabetes, bind 50, nr. 5, s. 1056-63.

Proteome analysis of interleukin-1beta-induced changes in protein expression in rat islets of Langerhans. / Larsen, P M; Fey, S J; Larsen, M R; Nawrocki, A; Andersen, H U; Kähler, H; Heilmann, C; Voss, M C; Roepstorff, P; Pociot, F; Karlsen, A E; Nerup, J.

I: Diabetes, Bind 50, Nr. 5, 01.05.2001, s. 1056-63.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Proteome analysis of interleukin-1beta-induced changes in protein expression in rat islets of Langerhans

AU - Larsen, P M

AU - Fey, S J

AU - Larsen, M R

AU - Nawrocki, A

AU - Andersen, H U

AU - Kähler, H

AU - Heilmann, C

AU - Voss, M C

AU - Roepstorff, P

AU - Pociot, F

AU - Karlsen, A E

AU - Nerup, J

PY - 2001/5/1

Y1 - 2001/5/1

N2 - The intracellular molecular events involved in the beta-cell death process are complex but poorly understood. Cytokines, e.g., interleukin (IL)-1beta, may play a crucial role in inducing this process. Protein synthesis is necessary for the deleterious effect of IL-1, and induction of both protective and deleterious proteins has been described. To characterize the rather complex pattern of islet protein expression in rat islets in response to IL-1, we have attempted to identify proteins of altered expression level after IL-1 exposure by 2D gel electrophoresis and mass spectrometry. Of 105 significantly changed (i.e., up- or downregulated or de novo-induced) protein spots, we obtained positive protein identification for 60 protein spots. The 60 identifications corresponded to 57 different proteins. Of these, 10 proteins were present in two to four spots, suggesting that posttranslatory modifications had occurred. In addition, 11 spots contained more than one protein. The proteins could be classified according to their function into the following groups: 1) energy transduction; 2) glycolytic pathway; 3) protein synthesis, chaperones, and protein folding; and 4) signal transduction, regulation, differentiation, and apoptosis. In conclusion, valuable information about the molecular mechanisms involved in cytokine-mediated beta-cell destruction was obtained by this approach.

AB - The intracellular molecular events involved in the beta-cell death process are complex but poorly understood. Cytokines, e.g., interleukin (IL)-1beta, may play a crucial role in inducing this process. Protein synthesis is necessary for the deleterious effect of IL-1, and induction of both protective and deleterious proteins has been described. To characterize the rather complex pattern of islet protein expression in rat islets in response to IL-1, we have attempted to identify proteins of altered expression level after IL-1 exposure by 2D gel electrophoresis and mass spectrometry. Of 105 significantly changed (i.e., up- or downregulated or de novo-induced) protein spots, we obtained positive protein identification for 60 protein spots. The 60 identifications corresponded to 57 different proteins. Of these, 10 proteins were present in two to four spots, suggesting that posttranslatory modifications had occurred. In addition, 11 spots contained more than one protein. The proteins could be classified according to their function into the following groups: 1) energy transduction; 2) glycolytic pathway; 3) protein synthesis, chaperones, and protein folding; and 4) signal transduction, regulation, differentiation, and apoptosis. In conclusion, valuable information about the molecular mechanisms involved in cytokine-mediated beta-cell destruction was obtained by this approach.

KW - Animals

KW - Cells, Cultured

KW - Electrophoresis, Gel, Two-Dimensional

KW - Energy Metabolism

KW - Gene Expression Regulation

KW - Interleukin-1

KW - Islets of Langerhans

KW - Mass Spectrometry

KW - Oxidation-Reduction

KW - Proteins

KW - Proteome

KW - Rats

M3 - Journal article

VL - 50

SP - 1056

EP - 1063

JO - Diabetes

JF - Diabetes

SN - 0012-1797

IS - 5

ER -

Larsen PM, Fey SJ, Larsen MR, Nawrocki A, Andersen HU, Kähler H et al. Proteome analysis of interleukin-1beta-induced changes in protein expression in rat islets of Langerhans. Diabetes. 2001 maj 1;50(5):1056-63.