Procollagen type I N-terminal propeptide (PINP) as an indicator of type I collagen metabolism: ELISA development, reference interval, and hypovitaminosis D induced hyperparathyroidism

O Orum, M Hansen, Charlotte Harken Jensen, H A Sørensen, L B Jensen, K Hørslev-Petersen, B Teisner

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

A sandwich enzyme-linked immunosorbent assay (ELISA) for quantification of the N-terminal propeptide of human procollagen type I (PINP) utilizing purified alpha 1-chain specific rabbit antibodies is described. The ELISA measured the content of the alpha 1-chain of PINP independent of the molecular form of the molecule. A parallelism was found between amniotic fluid (calibrator), normal and patient serum, and purified PINP (alpha 1), as well as the high and low molecular weight forms of PINP (alpha 1). The concentration of PINP in the calibrator (second trimester amniotic fluid) was determined to 25 micrograms/mL and the detection limit was 62 pg/mL measured in amniotic fluid, and 41 pg/mL measured in serum. The interassay coefficients of variation were 4.6% (low control) and 5.3% (high control), and the corresponding intraassay parameters were 2.9% and 4.9%. Recovery studies revealed an accuracy between 93% and 105%. The normal range (n = 57) for PINP was 56 ng/mL (median) the 10th and 90th centiles being 30 and 82 ng/mL, respectively. Patients with hyperparathyroidism due to hypovitaminosis D had median serum level of 168 ng/mL with a 10th centile of 44 ng/mL and a 90th centile of 450 ng/mL, these values being significantly different from the normal range (p < 0.001). The PINP-ELISA was superior to commercially available assays for PICP and osteocalcin in separation between healthy controls and patients with osteomalaci.
Udgivelsesdato: 1996-Aug
OriginalsprogEngelsk
TidsskriftBone
Vol/bind19
Udgave nummer2
Sider (fra-til)157-63
Antal sider6
ISSN8756-3282
StatusUdgivet - 1. aug. 1996

Citer dette

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title = "Procollagen type I N-terminal propeptide (PINP) as an indicator of type I collagen metabolism: ELISA development, reference interval, and hypovitaminosis D induced hyperparathyroidism",
abstract = "A sandwich enzyme-linked immunosorbent assay (ELISA) for quantification of the N-terminal propeptide of human procollagen type I (PINP) utilizing purified alpha 1-chain specific rabbit antibodies is described. The ELISA measured the content of the alpha 1-chain of PINP independent of the molecular form of the molecule. A parallelism was found between amniotic fluid (calibrator), normal and patient serum, and purified PINP (alpha 1), as well as the high and low molecular weight forms of PINP (alpha 1). The concentration of PINP in the calibrator (second trimester amniotic fluid) was determined to 25 micrograms/mL and the detection limit was 62 pg/mL measured in amniotic fluid, and 41 pg/mL measured in serum. The interassay coefficients of variation were 4.6{\%} (low control) and 5.3{\%} (high control), and the corresponding intraassay parameters were 2.9{\%} and 4.9{\%}. Recovery studies revealed an accuracy between 93{\%} and 105{\%}. The normal range (n = 57) for PINP was 56 ng/mL (median) the 10th and 90th centiles being 30 and 82 ng/mL, respectively. Patients with hyperparathyroidism due to hypovitaminosis D had median serum level of 168 ng/mL with a 10th centile of 44 ng/mL and a 90th centile of 450 ng/mL, these values being significantly different from the normal range (p < 0.001). The PINP-ELISA was superior to commercially available assays for PICP and osteocalcin in separation between healthy controls and patients with osteomalaci. Udgivelsesdato: 1996-Aug",
keywords = "Adult, Aged, Aged, 80 and over, Amniotic Fluid, Antibody Specificity, Biological Markers, Calibration, Case-Control Studies, Collagen, Enzyme-Linked Immunosorbent Assay, Female, Humans, Hyperparathyroidism, Male, Middle Aged, Peptide Fragments, Procollagen, Reference Values, Statistics as Topic, Time Factors, Vitamin D Deficiency",
author = "O Orum and M Hansen and Jensen, {Charlotte Harken} and S{\o}rensen, {H A} and Jensen, {L B} and K H{\o}rslev-Petersen and B Teisner",
year = "1996",
month = "8",
day = "1",
language = "English",
volume = "19",
pages = "157--63",
journal = "Bone",
issn = "8756-3282",
publisher = "Elsevier",
number = "2",

}

Procollagen type I N-terminal propeptide (PINP) as an indicator of type I collagen metabolism: ELISA development, reference interval, and hypovitaminosis D induced hyperparathyroidism. / Orum, O; Hansen, M; Jensen, Charlotte Harken; Sørensen, H A; Jensen, L B; Hørslev-Petersen, K; Teisner, B.

I: Bone, Bind 19, Nr. 2, 01.08.1996, s. 157-63.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Procollagen type I N-terminal propeptide (PINP) as an indicator of type I collagen metabolism: ELISA development, reference interval, and hypovitaminosis D induced hyperparathyroidism

AU - Orum, O

AU - Hansen, M

AU - Jensen, Charlotte Harken

AU - Sørensen, H A

AU - Jensen, L B

AU - Hørslev-Petersen, K

AU - Teisner, B

PY - 1996/8/1

Y1 - 1996/8/1

N2 - A sandwich enzyme-linked immunosorbent assay (ELISA) for quantification of the N-terminal propeptide of human procollagen type I (PINP) utilizing purified alpha 1-chain specific rabbit antibodies is described. The ELISA measured the content of the alpha 1-chain of PINP independent of the molecular form of the molecule. A parallelism was found between amniotic fluid (calibrator), normal and patient serum, and purified PINP (alpha 1), as well as the high and low molecular weight forms of PINP (alpha 1). The concentration of PINP in the calibrator (second trimester amniotic fluid) was determined to 25 micrograms/mL and the detection limit was 62 pg/mL measured in amniotic fluid, and 41 pg/mL measured in serum. The interassay coefficients of variation were 4.6% (low control) and 5.3% (high control), and the corresponding intraassay parameters were 2.9% and 4.9%. Recovery studies revealed an accuracy between 93% and 105%. The normal range (n = 57) for PINP was 56 ng/mL (median) the 10th and 90th centiles being 30 and 82 ng/mL, respectively. Patients with hyperparathyroidism due to hypovitaminosis D had median serum level of 168 ng/mL with a 10th centile of 44 ng/mL and a 90th centile of 450 ng/mL, these values being significantly different from the normal range (p < 0.001). The PINP-ELISA was superior to commercially available assays for PICP and osteocalcin in separation between healthy controls and patients with osteomalaci. Udgivelsesdato: 1996-Aug

AB - A sandwich enzyme-linked immunosorbent assay (ELISA) for quantification of the N-terminal propeptide of human procollagen type I (PINP) utilizing purified alpha 1-chain specific rabbit antibodies is described. The ELISA measured the content of the alpha 1-chain of PINP independent of the molecular form of the molecule. A parallelism was found between amniotic fluid (calibrator), normal and patient serum, and purified PINP (alpha 1), as well as the high and low molecular weight forms of PINP (alpha 1). The concentration of PINP in the calibrator (second trimester amniotic fluid) was determined to 25 micrograms/mL and the detection limit was 62 pg/mL measured in amniotic fluid, and 41 pg/mL measured in serum. The interassay coefficients of variation were 4.6% (low control) and 5.3% (high control), and the corresponding intraassay parameters were 2.9% and 4.9%. Recovery studies revealed an accuracy between 93% and 105%. The normal range (n = 57) for PINP was 56 ng/mL (median) the 10th and 90th centiles being 30 and 82 ng/mL, respectively. Patients with hyperparathyroidism due to hypovitaminosis D had median serum level of 168 ng/mL with a 10th centile of 44 ng/mL and a 90th centile of 450 ng/mL, these values being significantly different from the normal range (p < 0.001). The PINP-ELISA was superior to commercially available assays for PICP and osteocalcin in separation between healthy controls and patients with osteomalaci. Udgivelsesdato: 1996-Aug

KW - Adult

KW - Aged

KW - Aged, 80 and over

KW - Amniotic Fluid

KW - Antibody Specificity

KW - Biological Markers

KW - Calibration

KW - Case-Control Studies

KW - Collagen

KW - Enzyme-Linked Immunosorbent Assay

KW - Female

KW - Humans

KW - Hyperparathyroidism

KW - Male

KW - Middle Aged

KW - Peptide Fragments

KW - Procollagen

KW - Reference Values

KW - Statistics as Topic

KW - Time Factors

KW - Vitamin D Deficiency

M3 - Journal article

VL - 19

SP - 157

EP - 163

JO - Bone

JF - Bone

SN - 8756-3282

IS - 2

ER -