@inbook{4a25acc0951f4310abf937fe2445a7e4,
title = "Procedures for Derivation and Characterisation of Human Embryonic Stem Cells from Odense, Denmark",
abstract = "In 1998, a development occurred in stem cell biology with the fi rst report of the derivation of a human embryonic stem cell (hESC) line. Since then a number of techniques have been used to derive and characterise hESCs. Here, we describe the derivation methods used by our laboratory for isolation of the ICM by immunosurgery and outgrowth of the whole blastocyst. We have added protocols for routine culture, passaging and cryopreservation of our hESC lines as well as the methods we have used for characterisation (flow cytometry, karyotyping, immunocytochemistry, in vitro and in vivo differentiation). Additionally, we have included gene sequences for PCR and an antibody list for immunocytochemistry.",
keywords = "Human embryonic stem cells , Derivation, Characterisation",
author = "Linda Harkness and Moustapha Kassem",
year = "2012",
doi = "10.1007/978-1-61779-794-1\_3",
language = "English",
isbn = "978-1-61779-794-1",
series = "Methods in Molecular Medicine",
publisher = "Springer Science+Business Media",
pages = "33--51",
editor = "Kursad Turksen",
booktitle = "Human Embryonic Stem Cells Handbook",
address = "United States",
}