Procedures for Derivation and Characterisation of Human Embryonic Stem Cells from Odense, Denmark

Linda Harkness, Moustapha Kassem

Publikation: Kapitel i bog/rapport/konference-proceedingKapitel i bogForskning

Abstrakt

In 1998, a development occurred in stem cell biology with the fi rst report of the derivation of a human embryonic stem cell (hESC) line. Since then a number of techniques have been used to derive and characterise hESCs. Here, we describe the derivation methods used by our laboratory for isolation of the ICM by immunosurgery and outgrowth of the whole blastocyst. We have added protocols for routine culture, passaging and cryopreservation of our hESC lines as well as the methods we have used for characterisation (flow cytometry, karyotyping, immunocytochemistry, in vitro and in vivo differentiation). Additionally, we have included gene sequences for PCR and an antibody list for immunocytochemistry.
OriginalsprogEngelsk
TitelHuman Embryonic Stem Cells Handbook : Methods in Molecular Biology
RedaktørerKursad Turksen
ForlagSpringer Science+Business Media
Publikationsdato2012
Sider33-51
Kapitel3
ISBN (Trykt)978-1-61779-794-1
DOI
StatusUdgivet - 2012
NavnMethods in Molecular Medicine
Vol/bind873
ISSN1543-1894

Fingeraftryk

Dyk ned i forskningsemnerne om 'Procedures for Derivation and Characterisation of Human Embryonic Stem Cells from Odense, Denmark'. Sammen danner de et unikt fingeraftryk.

Citationsformater