Prenatal cytogenetic diagnosis after transabdominal chorionic villus sampling in the first trimester

A J Therkelsen, P K Jensen, Jens Michael Hertz, S Smidt-Jensen, N Hahnemann

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Abstrakt

First trimester prenatal cytogenetic diagnosis was attempted in 350 pregnancies after transabdominal chorionic villus sampling. The cytogenetic investigation was performed using both a short-term method (24 h incubation) and cell culture. Adequate samples were obtained in 99.1 per cent and in all these cases the fetal karyotype was established. A chromosome abnormality was found in 2.0 per cent of cases. A discrepancy between the karyotype obtained after 24 h incubation and the karyotype in cell culture was observed in 2.3 per cent. Maternal cell contamination in the cultures was confirmed in 13 of 181 cases where the 24 h incubation revealed a male karyotype. Studies of culture morphology showed that colonies of convoluted cells may serve as a marker for contamination with maternal cells in culture. For the present, we recommend using a short-term method as well as cell culture for cytogenetic investigation until the problems with karyotype discrepancy and maternal cell contamination have been further clarified.
OriginalsprogEngelsk
TidsskriftPrenatal Diagnosis
Vol/bind8
Udgave nummer1
Sider (fra-til)19-31
Antal sider13
ISSN0197-3851
StatusUdgivet - 1988

Fingeraftryk Dyk ned i forskningsemnerne om 'Prenatal cytogenetic diagnosis after transabdominal chorionic villus sampling in the first trimester'. Sammen danner de et unikt fingeraftryk.

Citationsformater