Polymerase-directed synthesis of C5-ethynyl locked nucleic acids

Rakesh N Veedu, Harsha Vardhan Reddy Burri, Pawan Kumar, Pawan K Sharma, Patrick J Hrdlicka, Birte Vester, Jesper Wengel

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Abstrakt

Modified nucleic acids have considerable potential in nanobiotechnology for the development of nanomedicines and new materials. Locked nucleic acid (LNA) is one of the most prominent nucleic acid analogues reported so far and we herein for the first time report the enzymatic incorporation of LNA-U and C5-ethynyl LNA-U nucleotides into oligonucleotides. Phusion High Fidelity and KOD DNA polymerases efficiently incorporated LNA-U and C5-ethynyl LNA-U nucleotides into a DNA strand and T7 RNA polymerase successfully accepted the LNA-U nucleoside 5'-triphosphate as substrate for RNA transcripts.
OriginalsprogEngelsk
TidsskriftBioorganic & Medicinal Chemistry Letters
Vol/bind20
Udgave nummer22
Sider (fra-til)6565-8
Antal sider4
ISSN0960-894X
DOI
StatusUdgivet - 15. nov. 2010

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