Polyaza crown ether as non-nucleosidic building blocks in DNA-conjugates

Ulla Jakobsen, Katja Rohr, Rasmus K Madsen, Stefan Vogel

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

 
OriginalsprogEngelsk
TidsskriftNucleosides, Nucleotides and Nucleic Acids
Vol/bind26
Udgave nummer10-12
Sider (fra-til)1221-1224
Antal sider3
ISSN1525-7770
DOI
StatusUdgivet - 2007

Fingeraftryk

Crown Ethers
Monomers
DNA
Denaturation
Oligonucleotides
Freezing
Melting
Thermodynamic stability
Stabilization
Experiments

Citer dette

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title = "Polyaza crown ether as non-nucleosidic building blocks in DNA-conjugates",
abstract = "The synthesis of amphiphilic polyaza crown ether monomers X (palmityl-substituted), Y (cholesteryl-substituted) and Z (dipalmityl-subtituted) and their incorporation into oligonucleotides are described. Their effects on thermal duplex stability were investigated by UV melting curve analysis. Thermal denaturation experiments showed remarkable stabilization of dsDNA by polyaza crown ether monomers when incorporated in opposite positions. The series of polyaza crown ether monomers (X, Y, and Z) with different lipophilicity showed a trend of increased stability of the corresponding dsDNA with increasing lipophilicity of the polyaza crown ether monomer.",
keywords = "Crown Ethers, DNA, Heat, Nucleic Acid Denaturation, Nucleosides, Oligonucleotides",
author = "Ulla Jakobsen and Katja Rohr and Madsen, {Rasmus K} and Stefan Vogel",
year = "2007",
doi = "10.1080/15257770701528149",
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Polyaza crown ether as non-nucleosidic building blocks in DNA-conjugates. / Jakobsen, Ulla; Rohr, Katja; Madsen, Rasmus K; Vogel, Stefan.

I: Nucleosides, Nucleotides and Nucleic Acids, Bind 26, Nr. 10-12, 2007, s. 1221-1224.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Polyaza crown ether as non-nucleosidic building blocks in DNA-conjugates

AU - Jakobsen, Ulla

AU - Rohr, Katja

AU - Madsen, Rasmus K

AU - Vogel, Stefan

PY - 2007

Y1 - 2007

N2 - The synthesis of amphiphilic polyaza crown ether monomers X (palmityl-substituted), Y (cholesteryl-substituted) and Z (dipalmityl-subtituted) and their incorporation into oligonucleotides are described. Their effects on thermal duplex stability were investigated by UV melting curve analysis. Thermal denaturation experiments showed remarkable stabilization of dsDNA by polyaza crown ether monomers when incorporated in opposite positions. The series of polyaza crown ether monomers (X, Y, and Z) with different lipophilicity showed a trend of increased stability of the corresponding dsDNA with increasing lipophilicity of the polyaza crown ether monomer.

AB - The synthesis of amphiphilic polyaza crown ether monomers X (palmityl-substituted), Y (cholesteryl-substituted) and Z (dipalmityl-subtituted) and their incorporation into oligonucleotides are described. Their effects on thermal duplex stability were investigated by UV melting curve analysis. Thermal denaturation experiments showed remarkable stabilization of dsDNA by polyaza crown ether monomers when incorporated in opposite positions. The series of polyaza crown ether monomers (X, Y, and Z) with different lipophilicity showed a trend of increased stability of the corresponding dsDNA with increasing lipophilicity of the polyaza crown ether monomer.

KW - Crown Ethers

KW - DNA

KW - Heat

KW - Nucleic Acid Denaturation

KW - Nucleosides

KW - Oligonucleotides

U2 - 10.1080/15257770701528149

DO - 10.1080/15257770701528149

M3 - Journal article

C2 - 18066756

VL - 26

SP - 1221

EP - 1224

JO - Nucleosides, Nucleotides and Nucleic Acids

JF - Nucleosides, Nucleotides and Nucleic Acids

SN - 1525-7770

IS - 10-12

ER -