Ploidy-stratified single cardiomyocyte transcriptomics map Zinc Finger E-Box Binding Homeobox 1 to underly cardiomyocyte proliferation before birth

Sara Thornby Bak, Eva Bang Harvald, Ditte Gry Ellman, Sabrina Bech Mathiesen, Ting Chen, Shu Fang, Kristian Skriver Andersen, Christina Dühring Fenger, Mark Burton, Mads Thomassen, Ditte Caroline Andersen*

*Kontaktforfatter

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

31 Downloads (Pure)

Abstract

Whereas cardiomyocytes (CMs) in the fetal heart divide, postnatal CMs fail to undergo karyokinesis and/or cytokinesis and therefore become polyploid or binucleated, a key process in terminal CM differentiation. This switch from a diploid proliferative CM to a terminally differentiated polyploid CM remains an enigma and seems an obstacle for heart regeneration. Here, we set out to identify the transcriptional landscape of CMs around birth using single cell RNA sequencing (scRNA-seq) to predict transcription factors (TFs) involved in CM proliferation and terminal differentiation. To this end, we established an approach combining fluorescence activated cell sorting (FACS) with scRNA-seq of fixed CMs from developing (E16.5, P1, and P5) mouse hearts, and generated high-resolution single-cell transcriptomic maps of in vivo diploid and tetraploid CMs, increasing the CM resolution. We identified TF-networks regulating the G2/M phases of developing CMs around birth. ZEB1 (Zinc Finger E-Box Binding Homeobox 1), a hereto unknown TF in CM cell cycling, was found to regulate the highest number of cell cycle genes in cycling CMs at E16.5 but was downregulated around birth. CM ZEB1-knockdown reduced proliferation of E16.5 CMs, while ZEB1 overexpression at P0 after birth resulted in CM endoreplication. These data thus provide a ploidy stratified transcriptomic map of developing CMs and bring new insight to CM proliferation and endoreplication identifying ZEB1 as a key player in these processes.

OriginalsprogEngelsk
Artikelnummer8
TidsskriftBasic Research in Cardiology
Vol/bind118
Udgave nummer1
ISSN0300-8428
DOI
StatusUdgivet - 2023

Bibliografisk note

Funding Information:
We would like to thank Charlotte Nielsen, Tina K. Andersen, and Tonja L. Jørgensen (Andersen group, Odense University Hospital) for excellent technical assistance on this study, Lars Vitved (University of Southern Denmark) for assisting with FACS and Professor Per Svenningsen (University of Southern Denmark) for help with AAV plasmids. The work was supported by research grants from The Novo Nordisk Foundation (#NNF17OC0028764), The Danish National Research Council (Sapere Aude; # 8045-00019B), The Lundbeck Foundation (#R313-2019-573), The Program of China Scholarship Council (No. 201906320411) and financial support from the Dep. of Clinical Biochemistry /Odense University Hospital.

Funding Information:
We would like to thank Charlotte Nielsen, Tina K. Andersen, and Tonja L. Jørgensen (Andersen group, Odense University Hospital) for excellent technical assistance on this study, Lars Vitved (University of Southern Denmark) for assisting with FACS and Professor Per Svenningsen (University of Southern Denmark) for help with AAV plasmids. The work was supported by research grants from The Novo Nordisk Foundation (#NNF17OC0028764), The Danish National Research Council (Sapere Aude; # 8045-00019B), The Lundbeck Foundation (#R313-2019-573), The Program of China Scholarship Council (No. 201906320411) and financial support from the Dep. of Clinical Biochemistry /Odense University Hospital.

Publisher Copyright:
© 2023, The Author(s).

Fingeraftryk

Dyk ned i forskningsemnerne om 'Ploidy-stratified single cardiomyocyte transcriptomics map Zinc Finger E-Box Binding Homeobox 1 to underly cardiomyocyte proliferation before birth'. Sammen danner de et unikt fingeraftryk.

Citationsformater