Placentation in cloned cattle

structure and microvascular architecture

M A Miglino, F T V Pereira, J A Visintin, J M Garcia, F V Meirelles, R Rumpf, C E Ambrósio, P C Papa, T C Santos, A F Carvalho, R Leiser, Anthony Michael Carter

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

 
Udgivelsesdato: 2007-Sep-1
OriginalsprogEngelsk
TidsskriftTheriogenology
Vol/bind68
Udgave nummer4
Sider (fra-til)604-617
Antal sider13
ISSN0093-691X
DOI
StatusUdgivet - 1. sep. 2007

Fingeraftryk

Placentation
placenta
Placenta
cattle
extraembryonic membranes
pregnancy
umbilical cord
Umbilical Cord
villi
Electron Scanning Microscopy
scanning electron microscopy
Chorioallantoic Membrane
chorioallantoic membrane
trophoblast
Trophoblasts
blood vessels
arteries
histology
Dilatation
epithelium

Citer dette

Miglino, M. A., Pereira, F. T. V., Visintin, J. A., Garcia, J. M., Meirelles, F. V., Rumpf, R., ... Carter, A. M. (2007). Placentation in cloned cattle: structure and microvascular architecture. Theriogenology, 68(4), 604-617. https://doi.org/10.1016/j.theriogenology.2007.04.060
Miglino, M A ; Pereira, F T V ; Visintin, J A ; Garcia, J M ; Meirelles, F V ; Rumpf, R ; Ambrósio, C E ; Papa, P C ; Santos, T C ; Carvalho, A F ; Leiser, R ; Carter, Anthony Michael. / Placentation in cloned cattle : structure and microvascular architecture. I: Theriogenology. 2007 ; Bind 68, Nr. 4. s. 604-617.
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title = "Placentation in cloned cattle: structure and microvascular architecture",
abstract = "To elucidate the morphological differences between placentas from normal and cloned cattle pregnancies reaching term, the umbilical cord, placentomes and interplacentomal region of the fetal membranes were examined macroscopically as well as by light and scanning electron microscopy. In pregnancies established by somatic nucleus transfer (NT), the umbilical cord and fetal membranes were edematous. Placentomal fusion was common, resulting in increased size and a decreased number of placentomes. Extensive areas of the chorioallantoic membrane were devoid of placentomes. An increased number of functional or accessory microcotyledons (<1 cm) were present at the maternally oriented surface of fetal membranes. Extensive areas of extravasated maternal blood were present within the placentomes and in the interplacentomal region. The crypts on the caruncular surface were dilated and accommodated complexes of more than one primary villus, as opposed to a single villus in non-cloned placentae. Scanning electron microscopy of blood vessel casts revealed that there was also more than one stem artery per villous tree and that the ramification of the vessels failed to form dense complexes of capillary loops and sinusoidal dilations as in normal pregnancies. At the materno-fetal interface, however, the trophoblast and uterine epithelium had normal histology. In conclusion, the NT placentas had a range of pathomorphological changes; this was likely associated with the poor clinical outcome of NT pregnancies.",
keywords = "Animals, Cattle, Cloning, Organism, Extraembryonic Membranes, Female, Male, Microscopy, Electron, Scanning, Nuclear Transfer Techniques, Placenta, Placentation, Pregnancy, Umbilical Cord",
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Miglino, MA, Pereira, FTV, Visintin, JA, Garcia, JM, Meirelles, FV, Rumpf, R, Ambrósio, CE, Papa, PC, Santos, TC, Carvalho, AF, Leiser, R & Carter, AM 2007, 'Placentation in cloned cattle: structure and microvascular architecture', Theriogenology, bind 68, nr. 4, s. 604-617. https://doi.org/10.1016/j.theriogenology.2007.04.060

Placentation in cloned cattle : structure and microvascular architecture. / Miglino, M A; Pereira, F T V; Visintin, J A; Garcia, J M; Meirelles, F V; Rumpf, R; Ambrósio, C E; Papa, P C; Santos, T C; Carvalho, A F; Leiser, R; Carter, Anthony Michael.

I: Theriogenology, Bind 68, Nr. 4, 01.09.2007, s. 604-617.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Placentation in cloned cattle

T2 - structure and microvascular architecture

AU - Miglino, M A

AU - Pereira, F T V

AU - Visintin, J A

AU - Garcia, J M

AU - Meirelles, F V

AU - Rumpf, R

AU - Ambrósio, C E

AU - Papa, P C

AU - Santos, T C

AU - Carvalho, A F

AU - Leiser, R

AU - Carter, Anthony Michael

PY - 2007/9/1

Y1 - 2007/9/1

N2 - To elucidate the morphological differences between placentas from normal and cloned cattle pregnancies reaching term, the umbilical cord, placentomes and interplacentomal region of the fetal membranes were examined macroscopically as well as by light and scanning electron microscopy. In pregnancies established by somatic nucleus transfer (NT), the umbilical cord and fetal membranes were edematous. Placentomal fusion was common, resulting in increased size and a decreased number of placentomes. Extensive areas of the chorioallantoic membrane were devoid of placentomes. An increased number of functional or accessory microcotyledons (<1 cm) were present at the maternally oriented surface of fetal membranes. Extensive areas of extravasated maternal blood were present within the placentomes and in the interplacentomal region. The crypts on the caruncular surface were dilated and accommodated complexes of more than one primary villus, as opposed to a single villus in non-cloned placentae. Scanning electron microscopy of blood vessel casts revealed that there was also more than one stem artery per villous tree and that the ramification of the vessels failed to form dense complexes of capillary loops and sinusoidal dilations as in normal pregnancies. At the materno-fetal interface, however, the trophoblast and uterine epithelium had normal histology. In conclusion, the NT placentas had a range of pathomorphological changes; this was likely associated with the poor clinical outcome of NT pregnancies.

AB - To elucidate the morphological differences between placentas from normal and cloned cattle pregnancies reaching term, the umbilical cord, placentomes and interplacentomal region of the fetal membranes were examined macroscopically as well as by light and scanning electron microscopy. In pregnancies established by somatic nucleus transfer (NT), the umbilical cord and fetal membranes were edematous. Placentomal fusion was common, resulting in increased size and a decreased number of placentomes. Extensive areas of the chorioallantoic membrane were devoid of placentomes. An increased number of functional or accessory microcotyledons (<1 cm) were present at the maternally oriented surface of fetal membranes. Extensive areas of extravasated maternal blood were present within the placentomes and in the interplacentomal region. The crypts on the caruncular surface were dilated and accommodated complexes of more than one primary villus, as opposed to a single villus in non-cloned placentae. Scanning electron microscopy of blood vessel casts revealed that there was also more than one stem artery per villous tree and that the ramification of the vessels failed to form dense complexes of capillary loops and sinusoidal dilations as in normal pregnancies. At the materno-fetal interface, however, the trophoblast and uterine epithelium had normal histology. In conclusion, the NT placentas had a range of pathomorphological changes; this was likely associated with the poor clinical outcome of NT pregnancies.

KW - Animals

KW - Cattle

KW - Cloning, Organism

KW - Extraembryonic Membranes

KW - Female

KW - Male

KW - Microscopy, Electron, Scanning

KW - Nuclear Transfer Techniques

KW - Placenta

KW - Placentation

KW - Pregnancy

KW - Umbilical Cord

U2 - 10.1016/j.theriogenology.2007.04.060

DO - 10.1016/j.theriogenology.2007.04.060

M3 - Journal article

VL - 68

SP - 604

EP - 617

JO - Theriogenology

JF - Theriogenology

SN - 0093-691X

IS - 4

ER -

Miglino MA, Pereira FTV, Visintin JA, Garcia JM, Meirelles FV, Rumpf R et al. Placentation in cloned cattle: structure and microvascular architecture. Theriogenology. 2007 sep 1;68(4):604-617. https://doi.org/10.1016/j.theriogenology.2007.04.060