Placental transport and in vitro effects of Bisphenol A

Thit J Mørck, Giuseppina Sorda, Nicoletta Bechi, Brian S Rasmussen, Jesper B Nielsen, Francesca Ietta, Erik Rytting, Line Mathiesen, Luana Paulesu, Lisbeth E Knudsen

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Udgivelsesdato: 2010-Aug
OriginalsprogEngelsk
TidsskriftReproductive toxicology (Elmsford, N.Y.)
Vol/bind30
Udgave nummer1
Sider (fra-til)131-7
Antal sider6
DOI
StatusUdgivet - 1. aug. 2010

Fingeraftryk

Placentation
Consumer products
Trophoblasts
Cytotoxicity
bisphenol A
In Vitro Techniques
Cell culture
Caspase 3
Risk assessment
Placenta
Toxicity
Monolayers
Skin
Estrogens
Perfusion
Cells
Cell Line

Citer dette

Mørck, T. J., Sorda, G., Bechi, N., Rasmussen, B. S., Nielsen, J. B., Ietta, F., ... Knudsen, L. E. (2010). Placental transport and in vitro effects of Bisphenol A. Reproductive toxicology (Elmsford, N.Y.), 30(1), 131-7. https://doi.org/10.1016/j.reprotox.2010.02.007
Mørck, Thit J ; Sorda, Giuseppina ; Bechi, Nicoletta ; Rasmussen, Brian S ; Nielsen, Jesper B ; Ietta, Francesca ; Rytting, Erik ; Mathiesen, Line ; Paulesu, Luana ; Knudsen, Lisbeth E. / Placental transport and in vitro effects of Bisphenol A. I: Reproductive toxicology (Elmsford, N.Y.). 2010 ; Bind 30, Nr. 1. s. 131-7.
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title = "Placental transport and in vitro effects of Bisphenol A",
abstract = "Bisphenol A (BPA), an estrogen-like chemical, leaches from consumer products potentially causing human exposure. To examine the effects of BPA exposure during pregnancy, we performed studies using the BeWo trophoblast cell line, placental explant cultures, placental perfusions and skin diffusion models, all of human origin. Results showed BPA cytotoxicity in BeWo cells with an apparent EC50 at 100-125 microM. BPA exposure significantly increased beta-hCG secretion and caspase-3 expression in placental explants at an environmentally relevant concentration of 1 nM. In the transport studies, a rapid transfer of BPA was observed across the term placentae and the BeWo cell monolayer. Further, transdermal transport of BPA was observed. These results indicate that fetal BPA exposure through placental exchange occurs with potential adverse implications for placental and fetal development. This battery of test systems within the realm of human implantation and fetal development represents important elements in risk assessment of reproductive toxicity.",
author = "M{\o}rck, {Thit J} and Giuseppina Sorda and Nicoletta Bechi and Rasmussen, {Brian S} and Nielsen, {Jesper B} and Francesca Ietta and Erik Rytting and Line Mathiesen and Luana Paulesu and Knudsen, {Lisbeth E}",
note = "Copyright 2010 Elsevier Inc. All rights reserved.",
year = "2010",
month = "8",
day = "1",
doi = "10.1016/j.reprotox.2010.02.007",
language = "English",
volume = "30",
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Mørck, TJ, Sorda, G, Bechi, N, Rasmussen, BS, Nielsen, JB, Ietta, F, Rytting, E, Mathiesen, L, Paulesu, L & Knudsen, LE 2010, 'Placental transport and in vitro effects of Bisphenol A', Reproductive toxicology (Elmsford, N.Y.), bind 30, nr. 1, s. 131-7. https://doi.org/10.1016/j.reprotox.2010.02.007

Placental transport and in vitro effects of Bisphenol A. / Mørck, Thit J; Sorda, Giuseppina; Bechi, Nicoletta; Rasmussen, Brian S; Nielsen, Jesper B; Ietta, Francesca; Rytting, Erik; Mathiesen, Line; Paulesu, Luana; Knudsen, Lisbeth E.

I: Reproductive toxicology (Elmsford, N.Y.), Bind 30, Nr. 1, 01.08.2010, s. 131-7.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Placental transport and in vitro effects of Bisphenol A

AU - Mørck, Thit J

AU - Sorda, Giuseppina

AU - Bechi, Nicoletta

AU - Rasmussen, Brian S

AU - Nielsen, Jesper B

AU - Ietta, Francesca

AU - Rytting, Erik

AU - Mathiesen, Line

AU - Paulesu, Luana

AU - Knudsen, Lisbeth E

N1 - Copyright 2010 Elsevier Inc. All rights reserved.

PY - 2010/8/1

Y1 - 2010/8/1

N2 - Bisphenol A (BPA), an estrogen-like chemical, leaches from consumer products potentially causing human exposure. To examine the effects of BPA exposure during pregnancy, we performed studies using the BeWo trophoblast cell line, placental explant cultures, placental perfusions and skin diffusion models, all of human origin. Results showed BPA cytotoxicity in BeWo cells with an apparent EC50 at 100-125 microM. BPA exposure significantly increased beta-hCG secretion and caspase-3 expression in placental explants at an environmentally relevant concentration of 1 nM. In the transport studies, a rapid transfer of BPA was observed across the term placentae and the BeWo cell monolayer. Further, transdermal transport of BPA was observed. These results indicate that fetal BPA exposure through placental exchange occurs with potential adverse implications for placental and fetal development. This battery of test systems within the realm of human implantation and fetal development represents important elements in risk assessment of reproductive toxicity.

AB - Bisphenol A (BPA), an estrogen-like chemical, leaches from consumer products potentially causing human exposure. To examine the effects of BPA exposure during pregnancy, we performed studies using the BeWo trophoblast cell line, placental explant cultures, placental perfusions and skin diffusion models, all of human origin. Results showed BPA cytotoxicity in BeWo cells with an apparent EC50 at 100-125 microM. BPA exposure significantly increased beta-hCG secretion and caspase-3 expression in placental explants at an environmentally relevant concentration of 1 nM. In the transport studies, a rapid transfer of BPA was observed across the term placentae and the BeWo cell monolayer. Further, transdermal transport of BPA was observed. These results indicate that fetal BPA exposure through placental exchange occurs with potential adverse implications for placental and fetal development. This battery of test systems within the realm of human implantation and fetal development represents important elements in risk assessment of reproductive toxicity.

U2 - 10.1016/j.reprotox.2010.02.007

DO - 10.1016/j.reprotox.2010.02.007

M3 - Journal article

VL - 30

SP - 131

EP - 137

JO - Reproductive Toxicology

JF - Reproductive Toxicology

SN - 0890-6238

IS - 1

ER -