Placental passage of benzoic acid, caffeine, and glyphosate in an ex vivo human perfusion system

Tina Mose, Mia Birkhoej Kjaerstad, Line Mathiesen, Jesper Bo Nielsen, Sven Edelfors, Lisbeth E Knudsen

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Ex vivo perfusion of the human term placenta is a method to study placental transfer without extrapolation from animal to human and with no ethical concerns for mother and child. However, ex vivo placenta perfusion has a limited potential within chemical screening and testing as the method is time-consuming. This study was an attempt to construct data needed to develop quantitative structure-activity relationship (QSAR) models that are able to predict placental transfer of new compounds. Placental transfer is a biological activity that statistically may be related to the physiochemical properties of a given group of compounds. Benzoic acid, caffeine, and glyphosate were chosen as model compounds because they are small molecules with large differences in physiochemical properties. Caffeine crossed the placenta by passive diffusion. The initial transfer rate of benzoic acid was more limited in the first part of the perfusion compared to caffeine, but reached the same steady-state level by the end of perfusion. The transfer of glyphosate was restricted throughout perfusion, with a lower permeation rate, and only around 15% glyphosate in maternal circulation crossed to the fetal circulation during the study period.
OriginalsprogEngelsk
TidsskriftJournal of Toxicology and Environmental Health. Part A
Vol/bind71
Udgave nummer15
Sider (fra-til)984-991
Antal sider7
ISSN1528-7394
DOI
StatusUdgivet - 2008

Fingeraftryk

glyphosate
Benzoic Acid
Caffeine
Perfusion
Placenta
Chemical potential
Bioactivity
Extrapolation
Permeation
Mothers
Screening
Animals
Quantitative Structure-Activity Relationship
Molecules
Testing

Citer dette

Mose, Tina ; Kjaerstad, Mia Birkhoej ; Mathiesen, Line ; Nielsen, Jesper Bo ; Edelfors, Sven ; Knudsen, Lisbeth E. / Placental passage of benzoic acid, caffeine, and glyphosate in an ex vivo human perfusion system. I: Journal of Toxicology and Environmental Health. Part A. 2008 ; Bind 71, Nr. 15. s. 984-991.
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abstract = "Ex vivo perfusion of the human term placenta is a method to study placental transfer without extrapolation from animal to human and with no ethical concerns for mother and child. However, ex vivo placenta perfusion has a limited potential within chemical screening and testing as the method is time-consuming. This study was an attempt to construct data needed to develop quantitative structure-activity relationship (QSAR) models that are able to predict placental transfer of new compounds. Placental transfer is a biological activity that statistically may be related to the physiochemical properties of a given group of compounds. Benzoic acid, caffeine, and glyphosate were chosen as model compounds because they are small molecules with large differences in physiochemical properties. Caffeine crossed the placenta by passive diffusion. The initial transfer rate of benzoic acid was more limited in the first part of the perfusion compared to caffeine, but reached the same steady-state level by the end of perfusion. The transfer of glyphosate was restricted throughout perfusion, with a lower permeation rate, and only around 15{\%} glyphosate in maternal circulation crossed to the fetal circulation during the study period.",
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Placental passage of benzoic acid, caffeine, and glyphosate in an ex vivo human perfusion system. / Mose, Tina; Kjaerstad, Mia Birkhoej; Mathiesen, Line; Nielsen, Jesper Bo; Edelfors, Sven; Knudsen, Lisbeth E.

I: Journal of Toxicology and Environmental Health. Part A, Bind 71, Nr. 15, 2008, s. 984-991.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Placental passage of benzoic acid, caffeine, and glyphosate in an ex vivo human perfusion system

AU - Mose, Tina

AU - Kjaerstad, Mia Birkhoej

AU - Mathiesen, Line

AU - Nielsen, Jesper Bo

AU - Edelfors, Sven

AU - Knudsen, Lisbeth E

PY - 2008

Y1 - 2008

N2 - Ex vivo perfusion of the human term placenta is a method to study placental transfer without extrapolation from animal to human and with no ethical concerns for mother and child. However, ex vivo placenta perfusion has a limited potential within chemical screening and testing as the method is time-consuming. This study was an attempt to construct data needed to develop quantitative structure-activity relationship (QSAR) models that are able to predict placental transfer of new compounds. Placental transfer is a biological activity that statistically may be related to the physiochemical properties of a given group of compounds. Benzoic acid, caffeine, and glyphosate were chosen as model compounds because they are small molecules with large differences in physiochemical properties. Caffeine crossed the placenta by passive diffusion. The initial transfer rate of benzoic acid was more limited in the first part of the perfusion compared to caffeine, but reached the same steady-state level by the end of perfusion. The transfer of glyphosate was restricted throughout perfusion, with a lower permeation rate, and only around 15% glyphosate in maternal circulation crossed to the fetal circulation during the study period.

AB - Ex vivo perfusion of the human term placenta is a method to study placental transfer without extrapolation from animal to human and with no ethical concerns for mother and child. However, ex vivo placenta perfusion has a limited potential within chemical screening and testing as the method is time-consuming. This study was an attempt to construct data needed to develop quantitative structure-activity relationship (QSAR) models that are able to predict placental transfer of new compounds. Placental transfer is a biological activity that statistically may be related to the physiochemical properties of a given group of compounds. Benzoic acid, caffeine, and glyphosate were chosen as model compounds because they are small molecules with large differences in physiochemical properties. Caffeine crossed the placenta by passive diffusion. The initial transfer rate of benzoic acid was more limited in the first part of the perfusion compared to caffeine, but reached the same steady-state level by the end of perfusion. The transfer of glyphosate was restricted throughout perfusion, with a lower permeation rate, and only around 15% glyphosate in maternal circulation crossed to the fetal circulation during the study period.

KW - Adult

KW - Benzoic Acid

KW - Caffeine

KW - Female

KW - Glycine

KW - Humans

KW - Maternal-Fetal Exchange

KW - Models, Biological

KW - Perfusion

KW - Placenta

KW - Pregnancy

KW - Quantitative Structure-Activity Relationship

U2 - 10.1080/01932690801934513

DO - 10.1080/01932690801934513

M3 - Journal article

VL - 71

SP - 984

EP - 991

JO - Journal of Toxicology and Environmental Health. Part A: Current Issues

JF - Journal of Toxicology and Environmental Health. Part A: Current Issues

SN - 1528-7394

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ER -