Physiological response in the European flounder (Platichthys flesus) to variable salinity and oxygen conditions

Kim Lundgreen, Pia Kiilerich, Christian Kølbæk Tipsmark, Steffen Madsen, Frank Bo Jensen

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

 

OriginalsprogEngelsk
TidsskriftJournal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology
Vol/bind178
Sider (fra-til)909-915
ISSN0174-1578
StatusUdgivet - 2008

Fingeraftryk

Flounder
Platichthys flesus
flounder
physiological response
hypoxia
Adenosine Triphosphatases
Oxygen
salinity
sodium-potassium-exchanging ATPase
oxygen
osmoregulation
normoxia
Blood
blood
Seawater
Nucleosides
Fish
Water content
Muscle
Energy conservation

Citer dette

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title = "Physiological response in the European flounder (Platichthys flesus) to variable salinity and oxygen conditions",
abstract = "Physiological mechanisms involved in acclimation to variable salinity and oxygen levels and their interaction were studied in European flounder. The fish were acclimated for two weeks to freshwater (1 ‰ salinity), brackish water (11 ‰) or full strength seawater (35 ‰) under normoxic conditions (water Po2 = 158 mmHg) and then subjected to 48 h of continued normoxia or hypoxia at a level (Po2 = 54 mmHg) close to but above the critical Po2. Plasma osmolality, [Na+] and [Cl-] increased with increasing salinity, but the rises were limited, reflecting an effective extracellular osmoregulation. Muscle water content was the same at all three salinities, indicating complete cell volume regulation. Gill Na+/K+-ATPase activity did not change with salinity, but hypoxia caused a 25 {\%} decrease in branchial Na+/K+-ATPase activity at all three salinities. Furthermore, hypoxia induced a significant decrease in mRNA levels of the Na+/K+-ATPase α1-subunit, signifying a reduced expression of the transporter gene. The reduced ATPase activity did not influence extracellular ionic concentrations. Blood [Hb] was stable with salinity, and it was not increased by hypoxia. Instead, hypoxia decreased the erythrocytic nucleoside triphosphate content, a common mechanism for increasing blood O2 affinity. It is concluded that moderate hypoxia induced an energy saving decrease in branchial Na+/K+-ATPase activity, which did not compromise extracellular osmoregulation.",
author = "Kim Lundgreen and Pia Kiilerich and Tipsmark, {Christian K{\o}lb{\ae}k} and Steffen Madsen and Jensen, {Frank Bo}",
year = "2008",
language = "English",
volume = "178",
pages = "909--915",
journal = "Journal of Comparative Physiology B: Biochemical, Systems, and Environmental Physiology",
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Physiological response in the European flounder (Platichthys flesus) to variable salinity and oxygen conditions. / Lundgreen, Kim; Kiilerich, Pia; Tipsmark, Christian Kølbæk; Madsen, Steffen; Jensen, Frank Bo.

I: Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology, Bind 178, 2008, s. 909-915.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Physiological response in the European flounder (Platichthys flesus) to variable salinity and oxygen conditions

AU - Lundgreen, Kim

AU - Kiilerich, Pia

AU - Tipsmark, Christian Kølbæk

AU - Madsen, Steffen

AU - Jensen, Frank Bo

PY - 2008

Y1 - 2008

N2 - Physiological mechanisms involved in acclimation to variable salinity and oxygen levels and their interaction were studied in European flounder. The fish were acclimated for two weeks to freshwater (1 ‰ salinity), brackish water (11 ‰) or full strength seawater (35 ‰) under normoxic conditions (water Po2 = 158 mmHg) and then subjected to 48 h of continued normoxia or hypoxia at a level (Po2 = 54 mmHg) close to but above the critical Po2. Plasma osmolality, [Na+] and [Cl-] increased with increasing salinity, but the rises were limited, reflecting an effective extracellular osmoregulation. Muscle water content was the same at all three salinities, indicating complete cell volume regulation. Gill Na+/K+-ATPase activity did not change with salinity, but hypoxia caused a 25 % decrease in branchial Na+/K+-ATPase activity at all three salinities. Furthermore, hypoxia induced a significant decrease in mRNA levels of the Na+/K+-ATPase α1-subunit, signifying a reduced expression of the transporter gene. The reduced ATPase activity did not influence extracellular ionic concentrations. Blood [Hb] was stable with salinity, and it was not increased by hypoxia. Instead, hypoxia decreased the erythrocytic nucleoside triphosphate content, a common mechanism for increasing blood O2 affinity. It is concluded that moderate hypoxia induced an energy saving decrease in branchial Na+/K+-ATPase activity, which did not compromise extracellular osmoregulation.

AB - Physiological mechanisms involved in acclimation to variable salinity and oxygen levels and their interaction were studied in European flounder. The fish were acclimated for two weeks to freshwater (1 ‰ salinity), brackish water (11 ‰) or full strength seawater (35 ‰) under normoxic conditions (water Po2 = 158 mmHg) and then subjected to 48 h of continued normoxia or hypoxia at a level (Po2 = 54 mmHg) close to but above the critical Po2. Plasma osmolality, [Na+] and [Cl-] increased with increasing salinity, but the rises were limited, reflecting an effective extracellular osmoregulation. Muscle water content was the same at all three salinities, indicating complete cell volume regulation. Gill Na+/K+-ATPase activity did not change with salinity, but hypoxia caused a 25 % decrease in branchial Na+/K+-ATPase activity at all three salinities. Furthermore, hypoxia induced a significant decrease in mRNA levels of the Na+/K+-ATPase α1-subunit, signifying a reduced expression of the transporter gene. The reduced ATPase activity did not influence extracellular ionic concentrations. Blood [Hb] was stable with salinity, and it was not increased by hypoxia. Instead, hypoxia decreased the erythrocytic nucleoside triphosphate content, a common mechanism for increasing blood O2 affinity. It is concluded that moderate hypoxia induced an energy saving decrease in branchial Na+/K+-ATPase activity, which did not compromise extracellular osmoregulation.

M3 - Journal article

VL - 178

SP - 909

EP - 915

JO - Journal of Comparative Physiology B: Biochemical, Systems, and Environmental Physiology

JF - Journal of Comparative Physiology B: Biochemical, Systems, and Environmental Physiology

SN - 0174-1578

ER -