Photophysical investigation of two emissive nucleosides exhibiting gigantic stokes shifts

Julie Stendevad, Mick Hornum, Daniel Wüstner, Jacob Kongsted*

*Kontaktforfatter for dette arbejde

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We report spectroscopic characterization of two emissive 2′-deoxycytidine analogues: 5-(5-phenylfuran-2-yl)-2′-deoxycytidine and 5-(1-phenyl-1H-pyrazol-3-yl)-2′-deoxycytidine. Their fluorescent properties were examined using a combined experimental and theory/simulation approach, where the latter was based on Born-Oppenheimer molecular dynamics and time-dependent density functional theory. The analogues were found to exhibit unusually large Stokes shifts in polar media (>100 nm), moderate fluorescence quantum yields, and their emissions were found to be very sensitive to the local dielectric environment. These two analogues of 2′-deoxycytidine thus hold a promising potential as probes in chemical biology. In addition, the accuracy of the theoretical models for determining the optical properties is validated, which opens up for a convenient way of assessing the potential of future probes.

TidsskriftPhotochemical and Photobiological Sciences
Udgave nummer7
Sider (fra-til)1858-1865
Antal sider8
StatusUdgivet - 17. jun. 2019

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