Photoligation of self-assembled DNA constructs containing anthracene-functionalized 2'-amino-LNA monomers

Karol Pasternak, Anna Pasternak, Pankaj Gupta, Rakesh N Veedu, Jesper Wengel

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Abstrakt

Efficient synthesis of a novel anthracene-functionalized 2'-amino-LNA phosphoramidite derivative is described together with its incorporation into oligodeoxynucleotides. Two DNA strands with the novel 2'-N-anthracenylmethyl-2'-amino-LNA monomers can be effectively cross-linked by photoligation at 366nm in various types of DNA constructs. Successful application of three differently functionalized 2'-amino-LNA monomers in self-assembled higher ordered structures for simultaneous cross-linking and monitoring of assembly formation is furthermore demonstrated.
OriginalsprogEngelsk
TidsskriftBioorganic & Medicinal Chemistry Letters
Vol/bind19
Udgave nummer24
Sider (fra-til)7407-15
Antal sider9
ISSN0960-894X
DOI
StatusUdgivet - 2011

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