Phosphate uptake kinetics for four species of submerged freshwater macrophytes measured by a 33P phosphate radioisotope technique

Nina H. Christiansen*, Frede O. Andersen, Henning S. Jensen

*Kontaktforfatter for dette arbejde

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

98 Downloads (Pure)

Resumé

Phosphate (Pi) uptake kinetics were determined in shoot and root tissues for four freshwater macrophyte species, Littorella uniflora, Potamogeton perfoliatus, Myriophyllum alterniflorum and Elodea canadensis, using a radioactive 33P phosphate technique. Collection of plant material in the oligotrophic softwater lake, Lake Hampen, Denmark, where Pi limits macrophyte growth, enabled us to characterize and compare the Pi uptake kinetics and competitive characteristics of the four species in a low level Pi environment. The maximum Pi uptake rates (Vmax), the half saturation constants (Km) together with the affinity at low Pi concentrations (Vmax/Km) were determined by fitting data to the Michaelis-Menten kinetics.L. uniflora showed the highest Vmax/Km in the root tissue and the lowest Km. M. alterniflorum showed the highest and E. canadensis and P. perfoliatus the lowest Vmax/Km in leaf tissue. M. alterniflorum had the highest Vmax and, as the only species, a higher Vmax in leaves than in roots. Surface area explained about half of Vmax in M. alterniflorum leaves. Roots were the dominant organ for Pi assimilation for all species at the Lake Hampen Pi concentrations. Km showed positive correlation to%P content in root tissue.The results indicate that at low lake water Pi concentrations L. uniflora is able to survive on the Pi pools in the sediment porewater. M. alterniflorum showed high affinity for Pi at both low and high Pi concentrations by both roots and shoots, and suggests that M. alterniflorum is a strong competitor at both low and high Pi concentrations.

OriginalsprogEngelsk
TidsskriftAquatic Botany
Vol/bind128
Sider (fra-til)58-67
Antal sider10
ISSN0304-3770
DOI
StatusUdgivet - 2016

Fingeraftryk

radionuclides
macrophytes
radionuclide
Littorella uniflora
phosphate
phosphates
uptake mechanisms
kinetics
Potamogeton perfoliatus
Elodea canadensis
lakes
macrophyte
lake
shoot
methodology
plant collections
Myriophyllum
leaves
enzyme kinetics
shoots

Citer dette

@article{4752e9938e8e41ecb9aa6146e191a3a3,
title = "Phosphate uptake kinetics for four species of submerged freshwater macrophytes measured by a 33P phosphate radioisotope technique",
abstract = "Phosphate (Pi) uptake kinetics were determined in shoot and root tissues for four freshwater macrophyte species, Littorella uniflora, Potamogeton perfoliatus, Myriophyllum alterniflorum and Elodea canadensis, using a radioactive 33P phosphate technique. Collection of plant material in the oligotrophic softwater lake, Lake Hampen, Denmark, where Pi limits macrophyte growth, enabled us to characterize and compare the Pi uptake kinetics and competitive characteristics of the four species in a low level Pi environment. The maximum Pi uptake rates (Vmax), the half saturation constants (Km) together with the affinity at low Pi concentrations (Vmax/Km) were determined by fitting data to the Michaelis-Menten kinetics.L. uniflora showed the highest Vmax/Km in the root tissue and the lowest Km. M. alterniflorum showed the highest and E. canadensis and P. perfoliatus the lowest Vmax/Km in leaf tissue. M. alterniflorum had the highest Vmax and, as the only species, a higher Vmax in leaves than in roots. Surface area explained about half of Vmax in M. alterniflorum leaves. Roots were the dominant organ for Pi assimilation for all species at the Lake Hampen Pi concentrations. Km showed positive correlation to{\%}P content in root tissue.The results indicate that at low lake water Pi concentrations L. uniflora is able to survive on the Pi pools in the sediment porewater. M. alterniflorum showed high affinity for Pi at both low and high Pi concentrations by both roots and shoots, and suggests that M. alterniflorum is a strong competitor at both low and high Pi concentrations.",
keywords = "Competition, Elodea canadensis, Lake, Littorella uniflora, Macrophytes, Myriophyllum alterniflorum, Nutrients, Oligotrophic, Potamogeton perfoliatus, Root-shoot ratio, Softwater",
author = "Christiansen, {Nina H.} and Andersen, {Frede O.} and Jensen, {Henning S.}",
year = "2016",
doi = "10.1016/j.aquabot.2015.10.002",
language = "English",
volume = "128",
pages = "58--67",
journal = "Aquatic Botany",
issn = "0304-3770",
publisher = "Elsevier",

}

Phosphate uptake kinetics for four species of submerged freshwater macrophytes measured by a 33P phosphate radioisotope technique. / Christiansen, Nina H.; Andersen, Frede O.; Jensen, Henning S.

I: Aquatic Botany, Bind 128, 2016, s. 58-67.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Phosphate uptake kinetics for four species of submerged freshwater macrophytes measured by a 33P phosphate radioisotope technique

AU - Christiansen, Nina H.

AU - Andersen, Frede O.

AU - Jensen, Henning S.

PY - 2016

Y1 - 2016

N2 - Phosphate (Pi) uptake kinetics were determined in shoot and root tissues for four freshwater macrophyte species, Littorella uniflora, Potamogeton perfoliatus, Myriophyllum alterniflorum and Elodea canadensis, using a radioactive 33P phosphate technique. Collection of plant material in the oligotrophic softwater lake, Lake Hampen, Denmark, where Pi limits macrophyte growth, enabled us to characterize and compare the Pi uptake kinetics and competitive characteristics of the four species in a low level Pi environment. The maximum Pi uptake rates (Vmax), the half saturation constants (Km) together with the affinity at low Pi concentrations (Vmax/Km) were determined by fitting data to the Michaelis-Menten kinetics.L. uniflora showed the highest Vmax/Km in the root tissue and the lowest Km. M. alterniflorum showed the highest and E. canadensis and P. perfoliatus the lowest Vmax/Km in leaf tissue. M. alterniflorum had the highest Vmax and, as the only species, a higher Vmax in leaves than in roots. Surface area explained about half of Vmax in M. alterniflorum leaves. Roots were the dominant organ for Pi assimilation for all species at the Lake Hampen Pi concentrations. Km showed positive correlation to%P content in root tissue.The results indicate that at low lake water Pi concentrations L. uniflora is able to survive on the Pi pools in the sediment porewater. M. alterniflorum showed high affinity for Pi at both low and high Pi concentrations by both roots and shoots, and suggests that M. alterniflorum is a strong competitor at both low and high Pi concentrations.

AB - Phosphate (Pi) uptake kinetics were determined in shoot and root tissues for four freshwater macrophyte species, Littorella uniflora, Potamogeton perfoliatus, Myriophyllum alterniflorum and Elodea canadensis, using a radioactive 33P phosphate technique. Collection of plant material in the oligotrophic softwater lake, Lake Hampen, Denmark, where Pi limits macrophyte growth, enabled us to characterize and compare the Pi uptake kinetics and competitive characteristics of the four species in a low level Pi environment. The maximum Pi uptake rates (Vmax), the half saturation constants (Km) together with the affinity at low Pi concentrations (Vmax/Km) were determined by fitting data to the Michaelis-Menten kinetics.L. uniflora showed the highest Vmax/Km in the root tissue and the lowest Km. M. alterniflorum showed the highest and E. canadensis and P. perfoliatus the lowest Vmax/Km in leaf tissue. M. alterniflorum had the highest Vmax and, as the only species, a higher Vmax in leaves than in roots. Surface area explained about half of Vmax in M. alterniflorum leaves. Roots were the dominant organ for Pi assimilation for all species at the Lake Hampen Pi concentrations. Km showed positive correlation to%P content in root tissue.The results indicate that at low lake water Pi concentrations L. uniflora is able to survive on the Pi pools in the sediment porewater. M. alterniflorum showed high affinity for Pi at both low and high Pi concentrations by both roots and shoots, and suggests that M. alterniflorum is a strong competitor at both low and high Pi concentrations.

KW - Competition

KW - Elodea canadensis

KW - Lake

KW - Littorella uniflora

KW - Macrophytes

KW - Myriophyllum alterniflorum

KW - Nutrients

KW - Oligotrophic

KW - Potamogeton perfoliatus

KW - Root-shoot ratio

KW - Softwater

UR - http://www.scopus.com/inward/record.url?scp=84945284081&partnerID=8YFLogxK

U2 - 10.1016/j.aquabot.2015.10.002

DO - 10.1016/j.aquabot.2015.10.002

M3 - Journal article

VL - 128

SP - 58

EP - 67

JO - Aquatic Botany

JF - Aquatic Botany

SN - 0304-3770

ER -