PaCATB, a secreted catalase protecting Podospora anserina against exogenous oxidative stress

Sandra Zintel, Dominik Bernhardt, Adelina Rogowska-Wrzesinska, Heinz D Osiewacz

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

A differential mass spectrometry analysis of secreted proteins from juvenile and senescentPodospora anserina cultures revealed age-related differences in protein profiles. Among other proteins with decreased abundance in the secretome of senescent cultures a catalase, termed PaCATB, was identified. Genetic modulation of the abundance of PaCATB identified differential effects on the phenotype of the corresponding strains. Deletion of PaCatB resulted in decreased resistance, over-expression in increased resistance against hydrogen peroxide. While the lifespan of the genetically modified strains was found to be unaffected under standard growth conditions, increased exogenous hydrogen peroxide stress in the growth medium markedly reduced the lifespan of the PaCatB deletion strain but extended the lifespan of PaCatB over-expressors. Overall our data identify a component of the secretome of P. anserina as a new effective factor to cope with environmental stress, stress that under natural conditions is constantly applied on organisms and influences aging processes.
OriginalsprogEngelsk
TidsskriftAging
Vol/bind3
Udgave nummer8
Sider (fra-til)768-781
ISSN1945-4589
DOI
StatusUdgivet - 2011

Fingeraftryk

Podospora
Hydrogen Peroxide
Proteins
Growth

Citer dette

Zintel, Sandra ; Bernhardt, Dominik ; Rogowska-Wrzesinska, Adelina ; Osiewacz, Heinz D. / PaCATB, a secreted catalase protecting Podospora anserina against exogenous oxidative stress. I: Aging. 2011 ; Bind 3, Nr. 8. s. 768-781.
@article{1059e736a6c74609807cbc54a0b6b918,
title = "PaCATB, a secreted catalase protecting Podospora anserina against exogenous oxidative stress",
abstract = "A differential mass spectrometry analysis of secreted proteins from juvenile and senescentPodospora anserina cultures revealed age-related differences in protein profiles. Among other proteins with decreased abundance in the secretome of senescent cultures a catalase, termed PaCATB, was identified. Genetic modulation of the abundance of PaCATB identified differential effects on the phenotype of the corresponding strains. Deletion of PaCatB resulted in decreased resistance, over-expression in increased resistance against hydrogen peroxide. While the lifespan of the genetically modified strains was found to be unaffected under standard growth conditions, increased exogenous hydrogen peroxide stress in the growth medium markedly reduced the lifespan of the PaCatB deletion strain but extended the lifespan of PaCatB over-expressors. Overall our data identify a component of the secretome of P. anserina as a new effective factor to cope with environmental stress, stress that under natural conditions is constantly applied on organisms and influences aging processes.",
author = "Sandra Zintel and Dominik Bernhardt and Adelina Rogowska-Wrzesinska and Osiewacz, {Heinz D}",
year = "2011",
doi = "10.18632/aging.100360",
language = "English",
volume = "3",
pages = "768--781",
journal = "Aging",
issn = "1945-4589",
publisher = "Impact Journals LLC",
number = "8",

}

PaCATB, a secreted catalase protecting Podospora anserina against exogenous oxidative stress. / Zintel, Sandra; Bernhardt, Dominik; Rogowska-Wrzesinska, Adelina; Osiewacz, Heinz D.

I: Aging, Bind 3, Nr. 8, 2011, s. 768-781.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - PaCATB, a secreted catalase protecting Podospora anserina against exogenous oxidative stress

AU - Zintel, Sandra

AU - Bernhardt, Dominik

AU - Rogowska-Wrzesinska, Adelina

AU - Osiewacz, Heinz D

PY - 2011

Y1 - 2011

N2 - A differential mass spectrometry analysis of secreted proteins from juvenile and senescentPodospora anserina cultures revealed age-related differences in protein profiles. Among other proteins with decreased abundance in the secretome of senescent cultures a catalase, termed PaCATB, was identified. Genetic modulation of the abundance of PaCATB identified differential effects on the phenotype of the corresponding strains. Deletion of PaCatB resulted in decreased resistance, over-expression in increased resistance against hydrogen peroxide. While the lifespan of the genetically modified strains was found to be unaffected under standard growth conditions, increased exogenous hydrogen peroxide stress in the growth medium markedly reduced the lifespan of the PaCatB deletion strain but extended the lifespan of PaCatB over-expressors. Overall our data identify a component of the secretome of P. anserina as a new effective factor to cope with environmental stress, stress that under natural conditions is constantly applied on organisms and influences aging processes.

AB - A differential mass spectrometry analysis of secreted proteins from juvenile and senescentPodospora anserina cultures revealed age-related differences in protein profiles. Among other proteins with decreased abundance in the secretome of senescent cultures a catalase, termed PaCATB, was identified. Genetic modulation of the abundance of PaCATB identified differential effects on the phenotype of the corresponding strains. Deletion of PaCatB resulted in decreased resistance, over-expression in increased resistance against hydrogen peroxide. While the lifespan of the genetically modified strains was found to be unaffected under standard growth conditions, increased exogenous hydrogen peroxide stress in the growth medium markedly reduced the lifespan of the PaCatB deletion strain but extended the lifespan of PaCatB over-expressors. Overall our data identify a component of the secretome of P. anserina as a new effective factor to cope with environmental stress, stress that under natural conditions is constantly applied on organisms and influences aging processes.

U2 - 10.18632/aging.100360

DO - 10.18632/aging.100360

M3 - Journal article

VL - 3

SP - 768

EP - 781

JO - Aging

JF - Aging

SN - 1945-4589

IS - 8

ER -