Ouabain-binding and 86rubidium-uptake in lymphocytes of normal and borderline hypertensive subjects

J R Nielsen, K E Pedersen, Torben Johansen, N A Klitgaard

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Resumé

In borderline hypertensives cellular sodium concentration seems to be increased, indicating that cellular abnormalities are present in the early course of essential hypertension. In order to study the mechanisms underlying this finding the number of sodium/potassium pump sites and the cation pump activity were studied in lymphocytes of nine borderline hypertensives (27 (20-36) years) and nine controls (28 (20-36) years). Maximum 3H-ouabain binding and 86Rb-uptake were taken as measures of the number of pump sites and cation pump activity, respectively. The median number of sodium/potassium pump sites was 49.6 X 10(3) molecules/cell in the BH group compared to 32.4 X 10(3) in the control group (P less than 0.01). Median 90 min 86Rb-uptakes were 54.0 pmol/10(6) cells in BH subjects and 39.4 in controls (P less than 0.10). The increased number of sodium/potassium pump sites and the tendency to increased cation pump activity in lymphocytes of BH subjects in vitro may be interpreted as an adaptive change possibly induced by a circulating natriuretic substance.
OriginalsprogEngelsk
TidsskriftScandinavian Journal of Clinical & Laboratory Investigation
Vol/bind43
Udgave nummer5
Sider (fra-til)393-9
Antal sider7
ISSN0036-5513
StatusUdgivet - 1983

Fingeraftryk

Sodium-Potassium-Exchanging ATPase
Ouabain
Control Groups

Citer dette

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title = "Ouabain-binding and 86rubidium-uptake in lymphocytes of normal and borderline hypertensive subjects",
abstract = "In borderline hypertensives cellular sodium concentration seems to be increased, indicating that cellular abnormalities are present in the early course of essential hypertension. In order to study the mechanisms underlying this finding the number of sodium/potassium pump sites and the cation pump activity were studied in lymphocytes of nine borderline hypertensives (27 (20-36) years) and nine controls (28 (20-36) years). Maximum 3H-ouabain binding and 86Rb-uptake were taken as measures of the number of pump sites and cation pump activity, respectively. The median number of sodium/potassium pump sites was 49.6 X 10(3) molecules/cell in the BH group compared to 32.4 X 10(3) in the control group (P less than 0.01). Median 90 min 86Rb-uptakes were 54.0 pmol/10(6) cells in BH subjects and 39.4 in controls (P less than 0.10). The increased number of sodium/potassium pump sites and the tendency to increased cation pump activity in lymphocytes of BH subjects in vitro may be interpreted as an adaptive change possibly induced by a circulating natriuretic substance.",
keywords = "Adult, Humans, Hypertension, Lymphocytes, Male, Ouabain, Potassium, Radioisotopes, Rubidium, Sodium",
author = "Nielsen, {J R} and Pedersen, {K E} and Torben Johansen and Klitgaard, {N A}",
year = "1983",
language = "English",
volume = "43",
pages = "393--9",
journal = "Scandinavian Journal of Clinical & Laboratory Investigation",
issn = "0036-5513",
publisher = "Taylor & Francis",
number = "5",

}

Ouabain-binding and 86rubidium-uptake in lymphocytes of normal and borderline hypertensive subjects. / Nielsen, J R; Pedersen, K E; Johansen, Torben; Klitgaard, N A.

I: Scandinavian Journal of Clinical & Laboratory Investigation, Bind 43, Nr. 5, 1983, s. 393-9.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Ouabain-binding and 86rubidium-uptake in lymphocytes of normal and borderline hypertensive subjects

AU - Nielsen, J R

AU - Pedersen, K E

AU - Johansen, Torben

AU - Klitgaard, N A

PY - 1983

Y1 - 1983

N2 - In borderline hypertensives cellular sodium concentration seems to be increased, indicating that cellular abnormalities are present in the early course of essential hypertension. In order to study the mechanisms underlying this finding the number of sodium/potassium pump sites and the cation pump activity were studied in lymphocytes of nine borderline hypertensives (27 (20-36) years) and nine controls (28 (20-36) years). Maximum 3H-ouabain binding and 86Rb-uptake were taken as measures of the number of pump sites and cation pump activity, respectively. The median number of sodium/potassium pump sites was 49.6 X 10(3) molecules/cell in the BH group compared to 32.4 X 10(3) in the control group (P less than 0.01). Median 90 min 86Rb-uptakes were 54.0 pmol/10(6) cells in BH subjects and 39.4 in controls (P less than 0.10). The increased number of sodium/potassium pump sites and the tendency to increased cation pump activity in lymphocytes of BH subjects in vitro may be interpreted as an adaptive change possibly induced by a circulating natriuretic substance.

AB - In borderline hypertensives cellular sodium concentration seems to be increased, indicating that cellular abnormalities are present in the early course of essential hypertension. In order to study the mechanisms underlying this finding the number of sodium/potassium pump sites and the cation pump activity were studied in lymphocytes of nine borderline hypertensives (27 (20-36) years) and nine controls (28 (20-36) years). Maximum 3H-ouabain binding and 86Rb-uptake were taken as measures of the number of pump sites and cation pump activity, respectively. The median number of sodium/potassium pump sites was 49.6 X 10(3) molecules/cell in the BH group compared to 32.4 X 10(3) in the control group (P less than 0.01). Median 90 min 86Rb-uptakes were 54.0 pmol/10(6) cells in BH subjects and 39.4 in controls (P less than 0.10). The increased number of sodium/potassium pump sites and the tendency to increased cation pump activity in lymphocytes of BH subjects in vitro may be interpreted as an adaptive change possibly induced by a circulating natriuretic substance.

KW - Adult

KW - Humans

KW - Hypertension

KW - Lymphocytes

KW - Male

KW - Ouabain

KW - Potassium

KW - Radioisotopes

KW - Rubidium

KW - Sodium

M3 - Journal article

C2 - 6648326

VL - 43

SP - 393

EP - 399

JO - Scandinavian Journal of Clinical & Laboratory Investigation

JF - Scandinavian Journal of Clinical & Laboratory Investigation

SN - 0036-5513

IS - 5

ER -