Osmoregulation and expression of ion transport proteins and putative claudins in the gill of southern flounder (Paralichthys lethostigma)

Christian K Tipsmark, J Adam Luckenbach, Steffen S Madsen, Pia Kiilerich, Russell J Borski

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Udgivelsesdato: 2008-Jul
OriginalsprogEngelsk
TidsskriftComparative Biochemistry and Physiology A
Vol/bind150
Udgave nummer3
Sider (fra-til)265-273
Antal sider8
ISSN1095-6433
DOI
StatusUdgivet - 1. jul. 2008

Fingeraftryk

Claudins
Flounder
Seawater
Fresh Water
Insulin-Like Growth Factor I
Claudin-3
Claudin-4
Tight Junction Proteins
Messenger RNA
Proteins
Liver
Vertebrates
Hormones
Muscles

Citer dette

@article{1624f990704111ddb1a1000ea68e967b,
title = "Osmoregulation and expression of ion transport proteins and putative claudins in the gill of southern flounder (Paralichthys lethostigma)",
abstract = "The southern flounder is a euryhaline teleost that inhabits ocean, estuarine, and riverine environments. We investigated the osmoregulatory strategy of juvenile flounder by examining the time-course of homeostatic responses, hormone levels, and gill Na(+),K(+)-ATPase and Na(+),K(+),2Cl(-) cotransporter protein expression after salinity challenge. Transfer of freshwater (FW)-acclimated flounder to sea water (SW) induced an increase in plasma osmolality and cortisol and a decrease in muscle water content, plasma insulin-like growth factor I (IGF-I) and hepatic IGF-I mRNA, all returning to control levels after 4 days. Gill Na(+),K(+)-ATPase and Na(+),K(+),2Cl(-) cotransporter protein levels were elevated in response to SW after 4 days. Transfer of SW-acclimated flounder to FW reduced gill Na(+),K(+)-ATPase and Na(+),K(+),2Cl(-) cotransporter protein, increased plasma IGF-I, but did not alter hepatic IGF-I mRNA or plasma cortisol levels. Gill claudin-3 and claudin-4 immunoreactive proteins were elevated in FW versus SW acclimated flounder. The study demonstrates that successful acclimation of southern flounder to SW or FW occurs after an initial crisis period and that the salinity adaptation process is associated with changes in branchial expression of ion transport and putative tight junction claudin proteins known to regulate epithelial permeability in mammalian vertebrates.",
keywords = "Animals, Blotting, Western, Cell Membrane, Fish Proteins, Flounder, Fresh Water, Gene Expression Regulation, Gills, Hydrocortisone, Insulin-Like Growth Factor I, Ion Transport, Liver, Membrane Proteins, Osmolar Concentration, RNA, Messenger, Seawater, Sodium-Potassium-Exchanging ATPase, Water-Electrolyte Balance",
author = "Tipsmark, {Christian K} and Luckenbach, {J Adam} and Madsen, {Steffen S} and Pia Kiilerich and Borski, {Russell J}",
year = "2008",
month = "7",
day = "1",
doi = "10.1016/j.cbpa.2008.03.006",
language = "English",
volume = "150",
pages = "265--273",
journal = "Comparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology",
issn = "1095-6433",
publisher = "Heinemann",
number = "3",

}

Osmoregulation and expression of ion transport proteins and putative claudins in the gill of southern flounder (Paralichthys lethostigma). / Tipsmark, Christian K; Luckenbach, J Adam; Madsen, Steffen S; Kiilerich, Pia; Borski, Russell J.

I: Comparative Biochemistry and Physiology A, Bind 150, Nr. 3, 01.07.2008, s. 265-273.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Osmoregulation and expression of ion transport proteins and putative claudins in the gill of southern flounder (Paralichthys lethostigma)

AU - Tipsmark, Christian K

AU - Luckenbach, J Adam

AU - Madsen, Steffen S

AU - Kiilerich, Pia

AU - Borski, Russell J

PY - 2008/7/1

Y1 - 2008/7/1

N2 - The southern flounder is a euryhaline teleost that inhabits ocean, estuarine, and riverine environments. We investigated the osmoregulatory strategy of juvenile flounder by examining the time-course of homeostatic responses, hormone levels, and gill Na(+),K(+)-ATPase and Na(+),K(+),2Cl(-) cotransporter protein expression after salinity challenge. Transfer of freshwater (FW)-acclimated flounder to sea water (SW) induced an increase in plasma osmolality and cortisol and a decrease in muscle water content, plasma insulin-like growth factor I (IGF-I) and hepatic IGF-I mRNA, all returning to control levels after 4 days. Gill Na(+),K(+)-ATPase and Na(+),K(+),2Cl(-) cotransporter protein levels were elevated in response to SW after 4 days. Transfer of SW-acclimated flounder to FW reduced gill Na(+),K(+)-ATPase and Na(+),K(+),2Cl(-) cotransporter protein, increased plasma IGF-I, but did not alter hepatic IGF-I mRNA or plasma cortisol levels. Gill claudin-3 and claudin-4 immunoreactive proteins were elevated in FW versus SW acclimated flounder. The study demonstrates that successful acclimation of southern flounder to SW or FW occurs after an initial crisis period and that the salinity adaptation process is associated with changes in branchial expression of ion transport and putative tight junction claudin proteins known to regulate epithelial permeability in mammalian vertebrates.

AB - The southern flounder is a euryhaline teleost that inhabits ocean, estuarine, and riverine environments. We investigated the osmoregulatory strategy of juvenile flounder by examining the time-course of homeostatic responses, hormone levels, and gill Na(+),K(+)-ATPase and Na(+),K(+),2Cl(-) cotransporter protein expression after salinity challenge. Transfer of freshwater (FW)-acclimated flounder to sea water (SW) induced an increase in plasma osmolality and cortisol and a decrease in muscle water content, plasma insulin-like growth factor I (IGF-I) and hepatic IGF-I mRNA, all returning to control levels after 4 days. Gill Na(+),K(+)-ATPase and Na(+),K(+),2Cl(-) cotransporter protein levels were elevated in response to SW after 4 days. Transfer of SW-acclimated flounder to FW reduced gill Na(+),K(+)-ATPase and Na(+),K(+),2Cl(-) cotransporter protein, increased plasma IGF-I, but did not alter hepatic IGF-I mRNA or plasma cortisol levels. Gill claudin-3 and claudin-4 immunoreactive proteins were elevated in FW versus SW acclimated flounder. The study demonstrates that successful acclimation of southern flounder to SW or FW occurs after an initial crisis period and that the salinity adaptation process is associated with changes in branchial expression of ion transport and putative tight junction claudin proteins known to regulate epithelial permeability in mammalian vertebrates.

KW - Animals

KW - Blotting, Western

KW - Cell Membrane

KW - Fish Proteins

KW - Flounder

KW - Fresh Water

KW - Gene Expression Regulation

KW - Gills

KW - Hydrocortisone

KW - Insulin-Like Growth Factor I

KW - Ion Transport

KW - Liver

KW - Membrane Proteins

KW - Osmolar Concentration

KW - RNA, Messenger

KW - Seawater

KW - Sodium-Potassium-Exchanging ATPase

KW - Water-Electrolyte Balance

U2 - 10.1016/j.cbpa.2008.03.006

DO - 10.1016/j.cbpa.2008.03.006

M3 - Journal article

VL - 150

SP - 265

EP - 273

JO - Comparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology

JF - Comparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology

SN - 1095-6433

IS - 3

ER -