Organotypic hippocampal slice cultures for studies of brain damage, neuroprotection and neurorepair

Jens Noraberg, Frantz Rom Poulsen, Morten Blaabjerg, Bjarne Winther Kristensen, Christian Bonde, Maria Montero, Morten Meyer, Jan Bert Gramsbergen, Jens Zimmer

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Udgivelsesdato: 2005-Aug
OriginalsprogEngelsk
TidsskriftCurrent Drug Targets. CNS & Neurological Disorders
Vol/bind4
Udgave nummer4
Sider (fra-til)435-452
Antal sider17
ISSN1568-007X
StatusUdgivet - 1. aug. 2005

Fingeraftryk

Alzheimer Disease
Excitatory Amino Acid Agonists
Propidium
Neurotoxins
Glutamate Receptors
Biosensing Techniques
Microelectrodes
Fluorescent Dyes
Fluorescence Microscopy
Neurodegenerative Diseases
Cell Movement
Theoretical Models
Clinical Trials
HIV
Oxygen
Neuroprotection
In Vitro Techniques

Citer dette

@article{785e3ac0fefb11dc86ef000ea68e967b,
title = "Organotypic hippocampal slice cultures for studies of brain damage, neuroprotection and neurorepair",
abstract = "Slices of developing brain tissue can be grown for several weeks as so-called organotypic slice cultures. Here we summarize and review studies using hippocampal slice cultures to investigate mechanisms and treatment strategies for the neurodegenerative disorders like stroke (cerebral ischemia), Alzheimer's disease (AD) and epilepsia. Studies of non-excitotoxic neurotoxic compounds and the experimental use of slice cultures in studies of HIV neurotoxicity, traumatic brain injury (TBI) and neurogenesis are included. For cerebral ischemia, experimental models with oxygen-glucose deprivation (OGD) and exposure to glutamate receptor agonists (excitotoxins) are reviewed. For epilepsia, focus is on induction of seizures with effects on neuronal loss, axonal sprouting and neurogenesis. For Alzheimer's disease, the review centers on the use of beta-amyloid (Abeta) in different models, while the section on repair is focused on neurogenesis and cell migration. The culturing techniques, set-up of models, and analytical tools, including markers for neurodegeneration, like the fluorescent dye propidium iodide (PI), are reviewed and discussed. Comparisons are made between hippocampal slice cultures and other in vitro models using dispersed cell cultures, experimental in vivo models, and in some instances, clinical trials. New techniques including slice culturing of hippocampal tissue from transgenic mice as well as more mature brain tissue, and slice cultures coupled to microelectrode arrays (MEAs), on-line biosensor monitoring, and time-lapse fluorescence microscopy are also presented.",
keywords = "Alzheimer Disease, Animals, Brain Damage, Chronic, Calcium, Disease Models, Animal, Epilepsy, Hippocampus, Mice, Nerve Degeneration, Neurodegenerative Diseases, Organ Culture Techniques, Rats, Signal Transduction, Stroke",
author = "Jens Noraberg and Poulsen, {Frantz Rom} and Morten Blaabjerg and Kristensen, {Bjarne Winther} and Christian Bonde and Maria Montero and Morten Meyer and Gramsbergen, {Jan Bert} and Jens Zimmer",
year = "2005",
month = "8",
day = "1",
language = "English",
volume = "4",
pages = "435--452",
journal = "C N S & Neurological Disorders - Drug Targets",
issn = "1871-5273",
publisher = "Bentham Science Publishers Ltd.",
number = "4",

}

Organotypic hippocampal slice cultures for studies of brain damage, neuroprotection and neurorepair. / Noraberg, Jens; Poulsen, Frantz Rom; Blaabjerg, Morten; Kristensen, Bjarne Winther; Bonde, Christian; Montero, Maria; Meyer, Morten; Gramsbergen, Jan Bert; Zimmer, Jens.

I: Current Drug Targets. CNS & Neurological Disorders, Bind 4, Nr. 4, 01.08.2005, s. 435-452.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Organotypic hippocampal slice cultures for studies of brain damage, neuroprotection and neurorepair

AU - Noraberg, Jens

AU - Poulsen, Frantz Rom

AU - Blaabjerg, Morten

AU - Kristensen, Bjarne Winther

AU - Bonde, Christian

AU - Montero, Maria

AU - Meyer, Morten

AU - Gramsbergen, Jan Bert

AU - Zimmer, Jens

PY - 2005/8/1

Y1 - 2005/8/1

N2 - Slices of developing brain tissue can be grown for several weeks as so-called organotypic slice cultures. Here we summarize and review studies using hippocampal slice cultures to investigate mechanisms and treatment strategies for the neurodegenerative disorders like stroke (cerebral ischemia), Alzheimer's disease (AD) and epilepsia. Studies of non-excitotoxic neurotoxic compounds and the experimental use of slice cultures in studies of HIV neurotoxicity, traumatic brain injury (TBI) and neurogenesis are included. For cerebral ischemia, experimental models with oxygen-glucose deprivation (OGD) and exposure to glutamate receptor agonists (excitotoxins) are reviewed. For epilepsia, focus is on induction of seizures with effects on neuronal loss, axonal sprouting and neurogenesis. For Alzheimer's disease, the review centers on the use of beta-amyloid (Abeta) in different models, while the section on repair is focused on neurogenesis and cell migration. The culturing techniques, set-up of models, and analytical tools, including markers for neurodegeneration, like the fluorescent dye propidium iodide (PI), are reviewed and discussed. Comparisons are made between hippocampal slice cultures and other in vitro models using dispersed cell cultures, experimental in vivo models, and in some instances, clinical trials. New techniques including slice culturing of hippocampal tissue from transgenic mice as well as more mature brain tissue, and slice cultures coupled to microelectrode arrays (MEAs), on-line biosensor monitoring, and time-lapse fluorescence microscopy are also presented.

AB - Slices of developing brain tissue can be grown for several weeks as so-called organotypic slice cultures. Here we summarize and review studies using hippocampal slice cultures to investigate mechanisms and treatment strategies for the neurodegenerative disorders like stroke (cerebral ischemia), Alzheimer's disease (AD) and epilepsia. Studies of non-excitotoxic neurotoxic compounds and the experimental use of slice cultures in studies of HIV neurotoxicity, traumatic brain injury (TBI) and neurogenesis are included. For cerebral ischemia, experimental models with oxygen-glucose deprivation (OGD) and exposure to glutamate receptor agonists (excitotoxins) are reviewed. For epilepsia, focus is on induction of seizures with effects on neuronal loss, axonal sprouting and neurogenesis. For Alzheimer's disease, the review centers on the use of beta-amyloid (Abeta) in different models, while the section on repair is focused on neurogenesis and cell migration. The culturing techniques, set-up of models, and analytical tools, including markers for neurodegeneration, like the fluorescent dye propidium iodide (PI), are reviewed and discussed. Comparisons are made between hippocampal slice cultures and other in vitro models using dispersed cell cultures, experimental in vivo models, and in some instances, clinical trials. New techniques including slice culturing of hippocampal tissue from transgenic mice as well as more mature brain tissue, and slice cultures coupled to microelectrode arrays (MEAs), on-line biosensor monitoring, and time-lapse fluorescence microscopy are also presented.

KW - Alzheimer Disease

KW - Animals

KW - Brain Damage, Chronic

KW - Calcium

KW - Disease Models, Animal

KW - Epilepsy

KW - Hippocampus

KW - Mice

KW - Nerve Degeneration

KW - Neurodegenerative Diseases

KW - Organ Culture Techniques

KW - Rats

KW - Signal Transduction

KW - Stroke

M3 - Journal article

C2 - 16101559

VL - 4

SP - 435

EP - 452

JO - C N S & Neurological Disorders - Drug Targets

JF - C N S & Neurological Disorders - Drug Targets

SN - 1871-5273

IS - 4

ER -