Optimisation of post mortem cardiac computed tomography compared to optical coherence tomography and histopathology - Technical note

Helle Precht*, Peter Mygind Leth, Jesper Thygesen, Michael Hardt-Madsen, Bjarne Nielsen, Erling Falk, Kenneth Egstrup, Oke Gerke, Alexander Broersen, Pieter H. Kitslaar, Jouke Dijkstra, Jess Lambrechtsen

*Kontaktforfatter for dette arbejde

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Introduction: Coronary atherosclerosis is a leading cause of mortality. New technological developments in computed tomography (CT), including dual energy, iterative reconstructions and high definition scanning, could significantly improve the non-invasive identification of atherosclerosis plaques. Here, a new method for optimising cardiac coronary CT with optical coherence tomography (OCT) and histopathology is presented. Materials and methods: Twenty human hearts obtained from autopsies were used. A contrast agent that solidifies after cooling was injected into the coronary arteries. CT scanning was performed on the heart alone as well as with the heart in a chest phantom. We used eight different CT protocols and the newest CT technique to image every heart. The OCT and CT images were compared with their corresponding histological sections. A procedure for ensuring the correct alignment of the images was also developed. Results: We have succeeded in developing a new method for post-mortem coronary CT angiography in which an autopsy heart is placed in a chest phantom to simulate clinical CT. Conclusion: The new method permits comparison of CT with OCT and histopathology. This method can also be used for evaluating coronary artery disease, including characterising plaques, and will eventually allow for the detection of rupture-prone plaques, which we will assess in a future study. Clinical testing is our ultimate goal.

OriginalsprogEngelsk
TidsskriftJournal of Forensic Radiology and Imaging
Vol/bind2
Udgave nummer2
Sider (fra-til)85-90
ISSN2212-4780
DOI
StatusUdgivet - 2014

Fingeraftryk

Optical Coherence Tomography
Coronary Artery Disease
Contrast Media
Rupture

Citer dette

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title = "Optimisation of post mortem cardiac computed tomography compared to optical coherence tomography and histopathology - Technical note",
abstract = "Introduction: Coronary atherosclerosis is a leading cause of mortality. New technological developments in computed tomography (CT), including dual energy, iterative reconstructions and high definition scanning, could significantly improve the non-invasive identification of atherosclerosis plaques. Here, a new method for optimising cardiac coronary CT with optical coherence tomography (OCT) and histopathology is presented. Materials and methods: Twenty human hearts obtained from autopsies were used. A contrast agent that solidifies after cooling was injected into the coronary arteries. CT scanning was performed on the heart alone as well as with the heart in a chest phantom. We used eight different CT protocols and the newest CT technique to image every heart. The OCT and CT images were compared with their corresponding histological sections. A procedure for ensuring the correct alignment of the images was also developed. Results: We have succeeded in developing a new method for post-mortem coronary CT angiography in which an autopsy heart is placed in a chest phantom to simulate clinical CT. Conclusion: The new method permits comparison of CT with OCT and histopathology. This method can also be used for evaluating coronary artery disease, including characterising plaques, and will eventually allow for the detection of rupture-prone plaques, which we will assess in a future study. Clinical testing is our ultimate goal.",
keywords = "Angiography, Atherosclerosis, Computerised tomography, Coronary artery disease, CT-scanning",
author = "Helle Precht and Leth, {Peter Mygind} and Jesper Thygesen and Michael Hardt-Madsen and Bjarne Nielsen and Erling Falk and Kenneth Egstrup and Oke Gerke and Alexander Broersen and Kitslaar, {Pieter H.} and Jouke Dijkstra and Jess Lambrechtsen",
year = "2014",
doi = "10.1016/j.jofri.2013.12.006",
language = "English",
volume = "2",
pages = "85--90",
journal = "Journal of Forensic Radiology and Imaging",
issn = "2212-4780",
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Optimisation of post mortem cardiac computed tomography compared to optical coherence tomography and histopathology - Technical note. / Precht, Helle; Leth, Peter Mygind; Thygesen, Jesper; Hardt-Madsen, Michael; Nielsen, Bjarne; Falk, Erling; Egstrup, Kenneth; Gerke, Oke; Broersen, Alexander; Kitslaar, Pieter H.; Dijkstra, Jouke; Lambrechtsen, Jess.

I: Journal of Forensic Radiology and Imaging, Bind 2, Nr. 2, 2014, s. 85-90.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Optimisation of post mortem cardiac computed tomography compared to optical coherence tomography and histopathology - Technical note

AU - Precht, Helle

AU - Leth, Peter Mygind

AU - Thygesen, Jesper

AU - Hardt-Madsen, Michael

AU - Nielsen, Bjarne

AU - Falk, Erling

AU - Egstrup, Kenneth

AU - Gerke, Oke

AU - Broersen, Alexander

AU - Kitslaar, Pieter H.

AU - Dijkstra, Jouke

AU - Lambrechtsen, Jess

PY - 2014

Y1 - 2014

N2 - Introduction: Coronary atherosclerosis is a leading cause of mortality. New technological developments in computed tomography (CT), including dual energy, iterative reconstructions and high definition scanning, could significantly improve the non-invasive identification of atherosclerosis plaques. Here, a new method for optimising cardiac coronary CT with optical coherence tomography (OCT) and histopathology is presented. Materials and methods: Twenty human hearts obtained from autopsies were used. A contrast agent that solidifies after cooling was injected into the coronary arteries. CT scanning was performed on the heart alone as well as with the heart in a chest phantom. We used eight different CT protocols and the newest CT technique to image every heart. The OCT and CT images were compared with their corresponding histological sections. A procedure for ensuring the correct alignment of the images was also developed. Results: We have succeeded in developing a new method for post-mortem coronary CT angiography in which an autopsy heart is placed in a chest phantom to simulate clinical CT. Conclusion: The new method permits comparison of CT with OCT and histopathology. This method can also be used for evaluating coronary artery disease, including characterising plaques, and will eventually allow for the detection of rupture-prone plaques, which we will assess in a future study. Clinical testing is our ultimate goal.

AB - Introduction: Coronary atherosclerosis is a leading cause of mortality. New technological developments in computed tomography (CT), including dual energy, iterative reconstructions and high definition scanning, could significantly improve the non-invasive identification of atherosclerosis plaques. Here, a new method for optimising cardiac coronary CT with optical coherence tomography (OCT) and histopathology is presented. Materials and methods: Twenty human hearts obtained from autopsies were used. A contrast agent that solidifies after cooling was injected into the coronary arteries. CT scanning was performed on the heart alone as well as with the heart in a chest phantom. We used eight different CT protocols and the newest CT technique to image every heart. The OCT and CT images were compared with their corresponding histological sections. A procedure for ensuring the correct alignment of the images was also developed. Results: We have succeeded in developing a new method for post-mortem coronary CT angiography in which an autopsy heart is placed in a chest phantom to simulate clinical CT. Conclusion: The new method permits comparison of CT with OCT and histopathology. This method can also be used for evaluating coronary artery disease, including characterising plaques, and will eventually allow for the detection of rupture-prone plaques, which we will assess in a future study. Clinical testing is our ultimate goal.

KW - Angiography

KW - Atherosclerosis

KW - Computerised tomography

KW - Coronary artery disease

KW - CT-scanning

U2 - 10.1016/j.jofri.2013.12.006

DO - 10.1016/j.jofri.2013.12.006

M3 - Journal article

VL - 2

SP - 85

EP - 90

JO - Journal of Forensic Radiology and Imaging

JF - Journal of Forensic Radiology and Imaging

SN - 2212-4780

IS - 2

ER -