Optimizing combination of vascular endothelial growth factor and mesenchymal stem cells on ectopic bone formation in SCID mice

Bidragets oversatte titel: Optimer kombinationen af vaskulært endothelial vækst faktor og mesenkymale stamceller på ektopisk knogleformation i SCID mus

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Abstrakt

Introduction: Insufficient blood supply may limit bone regeneration in bone defects. Vascular endothelial growth factor (VEGF) promotes angiogenesis by increasing endothelial migration. This outcome, however, could depend on time of application. Sheep mesenchymal stem cells (MSCs) in severe combined immunodeficient (SCID) mice were used in this study to evaluate optimal time points for VEGF stimulation to increase bone formation. Methods: Twenty-eight SCID (NOD.CB17-Prkdc scid/J) mice had hydroxyapatite granules seeded with 5 × 10 5 MSCs inserted subcutaneous. Pellets released VEGF on days 1–7, days 1–14, days 1–21, days 1–42, days 7–14, and days 21–42. After 8 weeks, the implant-bone-blocks were harvested, paraffin embedded, sectioned, and stained with both hematoxylin and eosin (HE) and immunohistochemistry for human vimentin (hVim) staining. Blood samples were collected for determination of bone-related biomarkers in serum. Results: The groups with 5 × 10 5 MSCs and VEGF stimulation on days 1–14 and days 1–21 showed more bone formation when compared to the control group of 5 × 10 5 MSCs alone (p < 0.01). Serum biomarkers had no significant values. The hVim staining confirmed the ovine origin of the observed ectopic bone formation. Conclusion: Optimal bone formation of MSCs was reached when stimulating with VEGF during the first 14 or 21 days after surgery.

Bidragets oversatte titelOptimer kombinationen af vaskulært endothelial vækst faktor og mesenkymale stamceller på ektopisk knogleformation i SCID mus
OriginalsprogEngelsk
TidsskriftJournal of Biomedical Materials Research. Part A
Vol/bind105
Udgave nummer12
Sider (fra-til)3326–3332
ISSN1549-3296
DOI
StatusUdgivet - dec. 2017

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