Resumé
| Originalsprog | Engelsk |
|---|---|
| Tidsskrift | Molecular Human Reproduction |
| Vol/bind | 17 |
| Udgave nummer | 12 |
| Sider (fra-til) | 758-61 |
| Antal sider | 4 |
| ISSN | 1360-9947 |
| DOI | |
| Status | Udgivet - 2011 |
Fingeraftryk
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Oncogenic events associated with endometrial and ovarian cancers are rare in endometriosis. / Vestergaard, Anna Lindeløv; Thorup, Katrine; Knudsen, Ulla B; Munk, Torben; Rosbach, Hanne; Poulsen, Jesper Bjørn Gorm; Guldberg, Per; Martensen, Pia Møller.
I: Molecular Human Reproduction, Bind 17, Nr. 12, 2011, s. 758-61.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review
TY - JOUR
T1 - Oncogenic events associated with endometrial and ovarian cancers are rare in endometriosis
AU - Vestergaard, Anna Lindeløv
AU - Thorup, Katrine
AU - Knudsen, Ulla B
AU - Munk, Torben
AU - Rosbach, Hanne
AU - Poulsen, Jesper Bjørn Gorm
AU - Guldberg, Per
AU - Martensen, Pia Møller
PY - 2011
Y1 - 2011
N2 - Endometriosis displays some features that resemble malignant processes, including invasive growth, resistance to apoptosis and distant implantation. The objective of this study was to investigate whether gene alterations that are frequent in endometrial and/or ovarian cancers contribute to the pathogenesis of endometriosis. Biopsies were obtained from ectopic endometriosis lesions from 23 patients with revised American Fertility Score stage 1 (n= 1), 2 (n= 10), 3 (n= 11) or 4 (n= 1) endometriosis. Six genes (APC, CDKN2A, PYCARD, RARB, RASSF1 and ESR1) were analyzed for promoter hypermethylation using methylation-specific melting curve analysis, and 9 genes (BRAF, HRAS, NRAS, CTNNB1, CDK4, FGFR3, PIK3CA, TP53 and PTEN) were analyzed for mutations using denaturing gradient gel electrophoresis and direct sequencing. An oncogenic mutation in KRAS (c.34G > T; p.G12C) was detected in a single lesion. No gene alterations were found in the remaining samples. Our data suggest that genetic and epigenetic events contributing to endometrial and ovarian cancers are rare in endometriosis. However, other proto-oncogenes and tumor suppressor genes should be tested for alterations in order to identify the molecular basis of the susceptibility of endometriosis to malignant transformation.
AB - Endometriosis displays some features that resemble malignant processes, including invasive growth, resistance to apoptosis and distant implantation. The objective of this study was to investigate whether gene alterations that are frequent in endometrial and/or ovarian cancers contribute to the pathogenesis of endometriosis. Biopsies were obtained from ectopic endometriosis lesions from 23 patients with revised American Fertility Score stage 1 (n= 1), 2 (n= 10), 3 (n= 11) or 4 (n= 1) endometriosis. Six genes (APC, CDKN2A, PYCARD, RARB, RASSF1 and ESR1) were analyzed for promoter hypermethylation using methylation-specific melting curve analysis, and 9 genes (BRAF, HRAS, NRAS, CTNNB1, CDK4, FGFR3, PIK3CA, TP53 and PTEN) were analyzed for mutations using denaturing gradient gel electrophoresis and direct sequencing. An oncogenic mutation in KRAS (c.34G > T; p.G12C) was detected in a single lesion. No gene alterations were found in the remaining samples. Our data suggest that genetic and epigenetic events contributing to endometrial and ovarian cancers are rare in endometriosis. However, other proto-oncogenes and tumor suppressor genes should be tested for alterations in order to identify the molecular basis of the susceptibility of endometriosis to malignant transformation.
U2 - 10.1093/molehr/gar049
DO - 10.1093/molehr/gar049
M3 - Journal article
C2 - 21724579
VL - 17
SP - 758
EP - 761
JO - Molecular Human Reproduction
JF - Molecular Human Reproduction
SN - 1360-9947
IS - 12
ER -