Oncogenic events associated with endometrial and ovarian cancers are rare in endometriosis

Anna Lindeløv Vestergaard, Katrine Thorup, Ulla B Knudsen, Torben Munk, Hanne Rosbach, Jesper Bjørn Gorm Poulsen, Per Guldberg, Pia Møller Martensen

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Endometriosis displays some features that resemble malignant processes, including invasive growth, resistance to apoptosis and distant implantation. The objective of this study was to investigate whether gene alterations that are frequent in endometrial and/or ovarian cancers contribute to the pathogenesis of endometriosis. Biopsies were obtained from ectopic endometriosis lesions from 23 patients with revised American Fertility Score stage 1 (n= 1), 2 (n= 10), 3 (n= 11) or 4 (n= 1) endometriosis. Six genes (APC, CDKN2A, PYCARD, RARB, RASSF1 and ESR1) were analyzed for promoter hypermethylation using methylation-specific melting curve analysis, and 9 genes (BRAF, HRAS, NRAS, CTNNB1, CDK4, FGFR3, PIK3CA, TP53 and PTEN) were analyzed for mutations using denaturing gradient gel electrophoresis and direct sequencing. An oncogenic mutation in KRAS (c.34G > T; p.G12C) was detected in a single lesion. No gene alterations were found in the remaining samples. Our data suggest that genetic and epigenetic events contributing to endometrial and ovarian cancers are rare in endometriosis. However, other proto-oncogenes and tumor suppressor genes should be tested for alterations in order to identify the molecular basis of the susceptibility of endometriosis to malignant transformation.
OriginalsprogEngelsk
TidsskriftMolecular Human Reproduction
Vol/bind17
Udgave nummer12
Sider (fra-til)758-61
Antal sider4
ISSN1360-9947
DOI
StatusUdgivet - 2011

Fingeraftryk

Endometriosis
Endometrial Neoplasms
Ovarian Neoplasms
APC Genes
p16 Genes
Mutation
Epigenomics
Freezing
Apoptosis
Growth

Citer dette

Vestergaard, A. L., Thorup, K., Knudsen, U. B., Munk, T., Rosbach, H., Poulsen, J. B. G., ... Martensen, P. M. (2011). Oncogenic events associated with endometrial and ovarian cancers are rare in endometriosis. Molecular Human Reproduction, 17(12), 758-61. https://doi.org/10.1093/molehr/gar049
Vestergaard, Anna Lindeløv ; Thorup, Katrine ; Knudsen, Ulla B ; Munk, Torben ; Rosbach, Hanne ; Poulsen, Jesper Bjørn Gorm ; Guldberg, Per ; Martensen, Pia Møller. / Oncogenic events associated with endometrial and ovarian cancers are rare in endometriosis. I: Molecular Human Reproduction. 2011 ; Bind 17, Nr. 12. s. 758-61.
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abstract = "Endometriosis displays some features that resemble malignant processes, including invasive growth, resistance to apoptosis and distant implantation. The objective of this study was to investigate whether gene alterations that are frequent in endometrial and/or ovarian cancers contribute to the pathogenesis of endometriosis. Biopsies were obtained from ectopic endometriosis lesions from 23 patients with revised American Fertility Score stage 1 (n= 1), 2 (n= 10), 3 (n= 11) or 4 (n= 1) endometriosis. Six genes (APC, CDKN2A, PYCARD, RARB, RASSF1 and ESR1) were analyzed for promoter hypermethylation using methylation-specific melting curve analysis, and 9 genes (BRAF, HRAS, NRAS, CTNNB1, CDK4, FGFR3, PIK3CA, TP53 and PTEN) were analyzed for mutations using denaturing gradient gel electrophoresis and direct sequencing. An oncogenic mutation in KRAS (c.34G > T; p.G12C) was detected in a single lesion. No gene alterations were found in the remaining samples. Our data suggest that genetic and epigenetic events contributing to endometrial and ovarian cancers are rare in endometriosis. However, other proto-oncogenes and tumor suppressor genes should be tested for alterations in order to identify the molecular basis of the susceptibility of endometriosis to malignant transformation.",
author = "Vestergaard, {Anna Lindel{\o}v} and Katrine Thorup and Knudsen, {Ulla B} and Torben Munk and Hanne Rosbach and Poulsen, {Jesper Bj{\o}rn Gorm} and Per Guldberg and Martensen, {Pia M{\o}ller}",
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Vestergaard, AL, Thorup, K, Knudsen, UB, Munk, T, Rosbach, H, Poulsen, JBG, Guldberg, P & Martensen, PM 2011, 'Oncogenic events associated with endometrial and ovarian cancers are rare in endometriosis', Molecular Human Reproduction, bind 17, nr. 12, s. 758-61. https://doi.org/10.1093/molehr/gar049

Oncogenic events associated with endometrial and ovarian cancers are rare in endometriosis. / Vestergaard, Anna Lindeløv; Thorup, Katrine; Knudsen, Ulla B; Munk, Torben; Rosbach, Hanne; Poulsen, Jesper Bjørn Gorm; Guldberg, Per; Martensen, Pia Møller.

I: Molecular Human Reproduction, Bind 17, Nr. 12, 2011, s. 758-61.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Oncogenic events associated with endometrial and ovarian cancers are rare in endometriosis

AU - Vestergaard, Anna Lindeløv

AU - Thorup, Katrine

AU - Knudsen, Ulla B

AU - Munk, Torben

AU - Rosbach, Hanne

AU - Poulsen, Jesper Bjørn Gorm

AU - Guldberg, Per

AU - Martensen, Pia Møller

PY - 2011

Y1 - 2011

N2 - Endometriosis displays some features that resemble malignant processes, including invasive growth, resistance to apoptosis and distant implantation. The objective of this study was to investigate whether gene alterations that are frequent in endometrial and/or ovarian cancers contribute to the pathogenesis of endometriosis. Biopsies were obtained from ectopic endometriosis lesions from 23 patients with revised American Fertility Score stage 1 (n= 1), 2 (n= 10), 3 (n= 11) or 4 (n= 1) endometriosis. Six genes (APC, CDKN2A, PYCARD, RARB, RASSF1 and ESR1) were analyzed for promoter hypermethylation using methylation-specific melting curve analysis, and 9 genes (BRAF, HRAS, NRAS, CTNNB1, CDK4, FGFR3, PIK3CA, TP53 and PTEN) were analyzed for mutations using denaturing gradient gel electrophoresis and direct sequencing. An oncogenic mutation in KRAS (c.34G > T; p.G12C) was detected in a single lesion. No gene alterations were found in the remaining samples. Our data suggest that genetic and epigenetic events contributing to endometrial and ovarian cancers are rare in endometriosis. However, other proto-oncogenes and tumor suppressor genes should be tested for alterations in order to identify the molecular basis of the susceptibility of endometriosis to malignant transformation.

AB - Endometriosis displays some features that resemble malignant processes, including invasive growth, resistance to apoptosis and distant implantation. The objective of this study was to investigate whether gene alterations that are frequent in endometrial and/or ovarian cancers contribute to the pathogenesis of endometriosis. Biopsies were obtained from ectopic endometriosis lesions from 23 patients with revised American Fertility Score stage 1 (n= 1), 2 (n= 10), 3 (n= 11) or 4 (n= 1) endometriosis. Six genes (APC, CDKN2A, PYCARD, RARB, RASSF1 and ESR1) were analyzed for promoter hypermethylation using methylation-specific melting curve analysis, and 9 genes (BRAF, HRAS, NRAS, CTNNB1, CDK4, FGFR3, PIK3CA, TP53 and PTEN) were analyzed for mutations using denaturing gradient gel electrophoresis and direct sequencing. An oncogenic mutation in KRAS (c.34G > T; p.G12C) was detected in a single lesion. No gene alterations were found in the remaining samples. Our data suggest that genetic and epigenetic events contributing to endometrial and ovarian cancers are rare in endometriosis. However, other proto-oncogenes and tumor suppressor genes should be tested for alterations in order to identify the molecular basis of the susceptibility of endometriosis to malignant transformation.

U2 - 10.1093/molehr/gar049

DO - 10.1093/molehr/gar049

M3 - Journal article

C2 - 21724579

VL - 17

SP - 758

EP - 761

JO - Molecular Human Reproduction

JF - Molecular Human Reproduction

SN - 1360-9947

IS - 12

ER -