OECD Endorsement of an AOP Network of 5 AOPs Linking Thyroid Hormone System Disruption to Impaired Swim Bladder Inflation in Fish

Lucia Vergauwen, Evelyn Stinckens, Daniel L. Villeneuve, Henrik Holbech, Lisa Baumann, Avril Hanbridge, Thomas Braunbeck, Dries Knapen

Publikation: Konferencebidrag uden forlag/tidsskriftPosterForskning


Over the last 10 years, through the success of several projects, five adverse outcome pathways (AOPs, AOP-Wiki IDs 155-159) have been developed linking thyroid hormone system disruption (THSD) to adverse effects on swim bladder inflation and survival in fish. The ECO20-UA project (2013-2016), funded by the Cefic Long-Range Research Initiative, investigated whether the AOP framework could provide a mechanistic basis for predicting chronic fish toxicity using non-animal alternatives. The AOPs were developed up to the point that they allowed selection of key events and development of alternative assays to identify thyroid hormone system disrupting chemicals (THSDCs). The follow-up ECO20.2 project (2016-2018) applied the alternative assays developed during ECO20 to a set of selected compounds, predicted acute and chronic toxicity, and performed Fish Embryo Acute Toxicity (FET) and chronic Fish Early-Life Stage (FELS) toxicity testing to validate the predictions. In the ERGO project (2019-2023), funded under the Horizon 2020 programme, the AOPs were completed and submitted for review by OECD. The AOPs have now been endorsed by the Working Group of the National Coordinators of the Test Guidelines program (WNT)/ Working Party on Hazard Assessment (WPHA) and will soon be published in the OECD Series on Adverse Outcome Pathways. We are now working on a broader network of THS AOPs to support cross-vertebrate extrapolation of mechanisms and effects of THSDCs for informing both on environmental and human health. Pre-validation of fish assays is also ongoing within ERGO. The endorsement and publication of these AOPs by OECD supports the addition of new endpoints to existing OECD fish Test Guidelines (TGs). This is facilitated by the close integration of AOP and TG development at the OECD level. Project 1.35 (Lead: BE, partners: US, DK, DE) on the OECD AOP development programme workplan overseen by EAGMST was initiated in 2015 and comprises the development of AOPs for THSD in fish. Project 2.64 (lead: DK, partners: BE, DE) on the OECD work plan for the Test Guidelines Programme overseen by VMG-Eco was initiated in 2019 and uses the AOPs as support for adding thyroid-sensitive endpoints to existing fish TGs. Such refined TGs will improve the evaluation of THSDCs and will be included in approaches for extrapolation of THSD effects across mammalian and amphibian species. The contents of this abstract neither constitute, nor necessarily reflect US EPA policy
Publikationsdato3. maj 2023
StatusUdgivet - 3. maj 2023
BegivenhedSETAC Europe 33rd Annual meeting: Data-driven environmental decision-making - the Convention Centre Dublin, Dublin, Irland
Varighed: 30. apr. 20234. maj 2023


KonferenceSETAC Europe 33rd Annual meeting
Lokation the Convention Centre Dublin


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