Nuclease resistant methylphosphonate-DNA/LNA chimeric oligonucleotides

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Abstrakt

Synthesis of chimeric 9-mer oligonucleotides containing methylphosphonate-linkages and locked nucleic acid (LNA) monomers, their binding affinity towards complementary DNA and RNA, and their 3′-exonucleolytic stability are described. The obtained methylphosphonate-DNA/LNA chimeric oligonucleotides display similarly high RNA affinity and RNA selectivity as a corresponding 9-mer DNA/LNA chimeric oligonucleotide, but much higher resistance towards 3′-exonucleolytic degradation.
OriginalsprogEngelsk
TidsskriftBioorganic & Medicinal Chemistry Letters
Vol/bind19
Udgave nummer10
Sider (fra-til)2707-2709
Antal sider3
ISSN0960-894X
DOI
StatusUdgivet - 2009

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