Resumé
BACKGROUND Zika virus (ZIKV) was recognised as a zoonotic pathogen in Africa and southeastern Asia. Human infections were infrequently reported until 2007, when the first known epidemic occurred in Micronesia. After 2013, the Asian lineage of ZIKV spread along the Pacific Islands and Americas, causing severe outbreaks with millions of human infections. The recent human infections of ZIKV were also associated with severe complications, such as an increase in cases of Guillain-Barre syndrome and the emergence of congenital Zika syndrome. OBJECTIVES To better understand the recent and rapid expansion of ZIKV, as well as the presentation of novel complications, we compared the genetic differences between the African sylvatic lineage and the Asian epidemic lineage that caused the recent massive outbreaks. FINDINGS The epidemic lineages have significant codon adaptation in NS1 gene to translate these proteins in human and Aedes aegypti mosquito cells compared to the African zoonotic lineage. Accordingly, a Brazilian epidemic isolate (ZBR) produced more NS1 protein than the MR766 African lineage (ZAF) did, as indicated by proteomic data from infections of neuron progenitor cells-derived neurospheres. Although ZBR replicated more efficiently in these cells, the differences observed in the stoichiometry of ZIKV proteins were not exclusively explained by the differences in viral replication between the lineages. MAIN CONCLUSIONS Our findings suggest that natural, silent translational selection in the second half of 20th century could have improved the fitness of Asian ZIKV lineage in human and mosquito cells.
| Originalsprog | Engelsk |
|---|---|
| Artikelnummer | e170385 |
| Tidsskrift | Memórias do Instituto Oswaldo Cruz |
| Vol/bind | 113 |
| Udgave nummer | 5 |
| ISSN | 0074-0276 |
| DOI | |
| Status | Udgivet - 2018 |
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NS1 codon usage adaptation to humans in pandemic Zika virus. / Freire, Caio César de Melo; Palmisano, Giuseppe; Braconi, Carla T.; Cugola, Fernanda R.; Russo, Fabiele B.; Beltrão-Braga, Patricia C.B.; Iamarino, Atila; de Lima Neto, Daniel Ferreira; Sall, Amadou Alpha; Rosa-Fernandes, Livia; Larsen, Martin R.; Zanotto, Paolo Marinho de Andrade.
I: Memórias do Instituto Oswaldo Cruz, Bind 113, Nr. 5, e170385, 2018.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review
TY - JOUR
T1 - NS1 codon usage adaptation to humans in pandemic Zika virus
AU - Freire, Caio César de Melo
AU - Palmisano, Giuseppe
AU - Braconi, Carla T.
AU - Cugola, Fernanda R.
AU - Russo, Fabiele B.
AU - Beltrão-Braga, Patricia C.B.
AU - Iamarino, Atila
AU - de Lima Neto, Daniel Ferreira
AU - Sall, Amadou Alpha
AU - Rosa-Fernandes, Livia
AU - Larsen, Martin R.
AU - Zanotto, Paolo Marinho de Andrade
PY - 2018
Y1 - 2018
N2 - BACKGROUND Zika virus (ZIKV) was recognised as a zoonotic pathogen in Africa and southeastern Asia. Human infections were infrequently reported until 2007, when the first known epidemic occurred in Micronesia. After 2013, the Asian lineage of ZIKV spread along the Pacific Islands and Americas, causing severe outbreaks with millions of human infections. The recent human infections of ZIKV were also associated with severe complications, such as an increase in cases of Guillain-Barre syndrome and the emergence of congenital Zika syndrome. OBJECTIVES To better understand the recent and rapid expansion of ZIKV, as well as the presentation of novel complications, we compared the genetic differences between the African sylvatic lineage and the Asian epidemic lineage that caused the recent massive outbreaks. FINDINGS The epidemic lineages have significant codon adaptation in NS1 gene to translate these proteins in human and Aedes aegypti mosquito cells compared to the African zoonotic lineage. Accordingly, a Brazilian epidemic isolate (ZBR) produced more NS1 protein than the MR766 African lineage (ZAF) did, as indicated by proteomic data from infections of neuron progenitor cells-derived neurospheres. Although ZBR replicated more efficiently in these cells, the differences observed in the stoichiometry of ZIKV proteins were not exclusively explained by the differences in viral replication between the lineages. MAIN CONCLUSIONS Our findings suggest that natural, silent translational selection in the second half of 20th century could have improved the fitness of Asian ZIKV lineage in human and mosquito cells.
AB - BACKGROUND Zika virus (ZIKV) was recognised as a zoonotic pathogen in Africa and southeastern Asia. Human infections were infrequently reported until 2007, when the first known epidemic occurred in Micronesia. After 2013, the Asian lineage of ZIKV spread along the Pacific Islands and Americas, causing severe outbreaks with millions of human infections. The recent human infections of ZIKV were also associated with severe complications, such as an increase in cases of Guillain-Barre syndrome and the emergence of congenital Zika syndrome. OBJECTIVES To better understand the recent and rapid expansion of ZIKV, as well as the presentation of novel complications, we compared the genetic differences between the African sylvatic lineage and the Asian epidemic lineage that caused the recent massive outbreaks. FINDINGS The epidemic lineages have significant codon adaptation in NS1 gene to translate these proteins in human and Aedes aegypti mosquito cells compared to the African zoonotic lineage. Accordingly, a Brazilian epidemic isolate (ZBR) produced more NS1 protein than the MR766 African lineage (ZAF) did, as indicated by proteomic data from infections of neuron progenitor cells-derived neurospheres. Although ZBR replicated more efficiently in these cells, the differences observed in the stoichiometry of ZIKV proteins were not exclusively explained by the differences in viral replication between the lineages. MAIN CONCLUSIONS Our findings suggest that natural, silent translational selection in the second half of 20th century could have improved the fitness of Asian ZIKV lineage in human and mosquito cells.
KW - Codon usage biases
KW - NS1 protein
KW - Proteomics
KW - Zika virus
U2 - 10.1590/0074-02760170385
DO - 10.1590/0074-02760170385
M3 - Journal article
C2 - 29768530
AN - SCOPUS:85046756768
VL - 113
JO - Memórias do Instituto Oswaldo Cruz
JF - Memórias do Instituto Oswaldo Cruz
SN - 0074-0276
IS - 5
M1 - e170385
ER -