Novel insights into the use of glowing LNA as nucleic acid detection probes-Influence of labeling density and nucleobases.

Michael E. Østergaard, Jyotirmoy Maity, B. Ravindra Babu, Jesper Wengel, Patrick J Hrdlicka

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Appropriately designed 2′-N-(pyren-1-yl)carbonyl-2′-amino-LNA (locked nucleic acid) display large increases in fluorescence intensity and remarkably high quantum yields upon hybridization with nucleic acid targets. Thermal denaturation and fluorescence spectroscopy studies on ONs modified with known thymine monomer X and novel 5-methylcytosine monomer Y provide new insights into the design principles and mechanism of these Glowing LNA nucleic acid detection probes.
OriginalsprogEngelsk
TidsskriftBioorganic & Medicinal Chemistry Letters
Vol/bind20
Udgave nummer24
Sider (fra-til)7265-7268
ISSN0960-894X
DOI
StatusUdgivet - 2010

Fingeraftryk

Nucleic Acid Probes
Labeling
Monomers
5-Methylcytosine
Denaturation
Thymine
Fluorescence spectroscopy
Quantum yield
Nucleic Acids
Fluorescence
locked nucleic acid
Hot Temperature
amino-LNA

Citer dette

Østergaard, Michael E. ; Maity, Jyotirmoy ; Babu, B. Ravindra ; Wengel, Jesper ; Hrdlicka, Patrick J. / Novel insights into the use of glowing LNA as nucleic acid detection probes-Influence of labeling density and nucleobases. I: Bioorganic & Medicinal Chemistry Letters. 2010 ; Bind 20, Nr. 24. s. 7265-7268.
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abstract = "Appropriately designed 2′-N-(pyren-1-yl)carbonyl-2′-amino-LNA (locked nucleic acid) display large increases in fluorescence intensity and remarkably high quantum yields upon hybridization with nucleic acid targets. Thermal denaturation and fluorescence spectroscopy studies on ONs modified with known thymine monomer X and novel 5-methylcytosine monomer Y provide new insights into the design principles and mechanism of these Glowing LNA nucleic acid detection probes.",
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Novel insights into the use of glowing LNA as nucleic acid detection probes-Influence of labeling density and nucleobases. / Østergaard, Michael E.; Maity, Jyotirmoy; Babu, B. Ravindra; Wengel, Jesper; Hrdlicka, Patrick J.

I: Bioorganic & Medicinal Chemistry Letters, Bind 20, Nr. 24, 2010, s. 7265-7268.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Novel insights into the use of glowing LNA as nucleic acid detection probes-Influence of labeling density and nucleobases.

AU - Østergaard, Michael E.

AU - Maity, Jyotirmoy

AU - Babu, B. Ravindra

AU - Wengel, Jesper

AU - Hrdlicka, Patrick J

PY - 2010

Y1 - 2010

N2 - Appropriately designed 2′-N-(pyren-1-yl)carbonyl-2′-amino-LNA (locked nucleic acid) display large increases in fluorescence intensity and remarkably high quantum yields upon hybridization with nucleic acid targets. Thermal denaturation and fluorescence spectroscopy studies on ONs modified with known thymine monomer X and novel 5-methylcytosine monomer Y provide new insights into the design principles and mechanism of these Glowing LNA nucleic acid detection probes.

AB - Appropriately designed 2′-N-(pyren-1-yl)carbonyl-2′-amino-LNA (locked nucleic acid) display large increases in fluorescence intensity and remarkably high quantum yields upon hybridization with nucleic acid targets. Thermal denaturation and fluorescence spectroscopy studies on ONs modified with known thymine monomer X and novel 5-methylcytosine monomer Y provide new insights into the design principles and mechanism of these Glowing LNA nucleic acid detection probes.

U2 - 10.1016/j.bmcl.2010.10.089

DO - 10.1016/j.bmcl.2010.10.089

M3 - Journal article

VL - 20

SP - 7265

EP - 7268

JO - Bioorganic & Medicinal Chemistry Letters

JF - Bioorganic & Medicinal Chemistry Letters

SN - 0960-894X

IS - 24

ER -