Novel characterisation of the gene encoding conglutinin reveals that previously characterised promoter corresponds to the CL-43 promoter

Soren Hansen, Vivi Moeller, Dorte Holm, Lars Vitved, Christian Bendixen, Karsten Skjødt, Uffe Holmskov

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Conglutinin, a collectin found in bovine serum, is an opsonin that binds to glycoconjugates on the surface of microorganisms or on deposited iC3b, and acts in concert with phagocytes to establish a first-line of immune defense. We have isolated a genomic conglutinin phage clone and found that the 5'-flanking region shows 95.8% identity with the sequence previously published, which on the other hand shows 99.7% identity with the CL-43 promoter. We conclude that the previously published promoter corresponds to the CL-43 promoter and that the functional studies performed on it apply to transcription of CL-43. Comparison of potential cis-regulatory elements in relation to the functional studies indicates that the two genes are regulated by different mechanisms.
OriginalsprogEngelsk
TidsskriftMolecular Immunology
Vol/bind39
Udgave nummer1-2
Sider (fra-til)39-43
Antal sider4
ISSN0161-5890
StatusUdgivet - 1. sep. 2002

Fingeraftryk

Opsonin Proteins
5' Flanking Region
Phagocytes
Clone Cells
Serum
conglutinin

Citer dette

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title = "Novel characterisation of the gene encoding conglutinin reveals that previously characterised promoter corresponds to the CL-43 promoter",
abstract = "Conglutinin, a collectin found in bovine serum, is an opsonin that binds to glycoconjugates on the surface of microorganisms or on deposited iC3b, and acts in concert with phagocytes to establish a first-line of immune defense. We have isolated a genomic conglutinin phage clone and found that the 5'-flanking region shows 95.8{\%} identity with the sequence previously published, which on the other hand shows 99.7{\%} identity with the CL-43 promoter. We conclude that the previously published promoter corresponds to the CL-43 promoter and that the functional studies performed on it apply to transcription of CL-43. Comparison of potential cis-regulatory elements in relation to the functional studies indicates that the two genes are regulated by different mechanisms.",
keywords = "Base Sequence, Collectins, Molecular Sequence Data, Promoter Regions, Genetic, Serum Globulins",
author = "Soren Hansen and Vivi Moeller and Dorte Holm and Lars Vitved and Christian Bendixen and Karsten Skj{\o}dt and Uffe Holmskov",
year = "2002",
month = "9",
day = "1",
language = "English",
volume = "39",
pages = "39--43",
journal = "Molecular Immunology",
issn = "0161-5890",
publisher = "Pergamon Press",
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Novel characterisation of the gene encoding conglutinin reveals that previously characterised promoter corresponds to the CL-43 promoter. / Hansen, Soren; Moeller, Vivi; Holm, Dorte; Vitved, Lars; Bendixen, Christian; Skjødt, Karsten; Holmskov, Uffe.

I: Molecular Immunology, Bind 39, Nr. 1-2, 01.09.2002, s. 39-43.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Novel characterisation of the gene encoding conglutinin reveals that previously characterised promoter corresponds to the CL-43 promoter

AU - Hansen, Soren

AU - Moeller, Vivi

AU - Holm, Dorte

AU - Vitved, Lars

AU - Bendixen, Christian

AU - Skjødt, Karsten

AU - Holmskov, Uffe

PY - 2002/9/1

Y1 - 2002/9/1

N2 - Conglutinin, a collectin found in bovine serum, is an opsonin that binds to glycoconjugates on the surface of microorganisms or on deposited iC3b, and acts in concert with phagocytes to establish a first-line of immune defense. We have isolated a genomic conglutinin phage clone and found that the 5'-flanking region shows 95.8% identity with the sequence previously published, which on the other hand shows 99.7% identity with the CL-43 promoter. We conclude that the previously published promoter corresponds to the CL-43 promoter and that the functional studies performed on it apply to transcription of CL-43. Comparison of potential cis-regulatory elements in relation to the functional studies indicates that the two genes are regulated by different mechanisms.

AB - Conglutinin, a collectin found in bovine serum, is an opsonin that binds to glycoconjugates on the surface of microorganisms or on deposited iC3b, and acts in concert with phagocytes to establish a first-line of immune defense. We have isolated a genomic conglutinin phage clone and found that the 5'-flanking region shows 95.8% identity with the sequence previously published, which on the other hand shows 99.7% identity with the CL-43 promoter. We conclude that the previously published promoter corresponds to the CL-43 promoter and that the functional studies performed on it apply to transcription of CL-43. Comparison of potential cis-regulatory elements in relation to the functional studies indicates that the two genes are regulated by different mechanisms.

KW - Base Sequence

KW - Collectins

KW - Molecular Sequence Data

KW - Promoter Regions, Genetic

KW - Serum Globulins

M3 - Journal article

VL - 39

SP - 39

EP - 43

JO - Molecular Immunology

JF - Molecular Immunology

SN - 0161-5890

IS - 1-2

ER -