Non-covalent encapsulation of siRNA with cell-penetrating peptides

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Abstract

SiRNAs may act as selective and potent therapeutics, but poor deliverability in vivo is a limitation. Among the recently proposed vectors, cell-penetrating peptides (CPPs), also referred as protein transduction domains (PTDs), allow siRNA stabilization and increased cellular uptake. This chapter aims to guide scientists in the preparation and characterization of CPP-siRNA complexes, particularly the evaluation of novel CPPs variants for siRNA encapsulation and delivery. Herein, we present a collection of methods to determine CPP-siRNA interaction, encapsulation, stability, conformation, transfection, and silencing efficiency.

OriginalsprogEngelsk
TitelDesign and Delivery of SiRNA Therapeutics
RedaktørerHenrik J. Ditzel, Martina Tuttolomondo, Sakari Kauppinen
Vol/bind2282
ForlagHumana Press
Publikationsdato2021
Sider353-376
ISBN (Trykt)978-1-0716-1297-2
ISBN (Elektronisk)978-1-0716-1298-9
DOI
StatusUdgivet - 2021
NavnMethods in Molecular Biology
Vol/bind2282
ISSN1064-3745

Bibliografisk note

Funding Information:
We gratefully acknowledge funding support from the European Union PathChooser Marie-Curie Initial Training Network (ITN) (PITN-GA-2013-608373), the Lundbeckfonden Center of Excellence NanoCAN, the University of Southern Denmark (SDU) DAWN-2020 project of the SDU Presidents SDU2020 program, the Danish Cancer Society, A Race Agaisnt Breast Cancer and the A.P. Moeller Foundation. Moreover, we acknowledge the Danish Molecular Biomedical Imaging Center (DaMBIC; University of Southern Denmark) for the use of the bioimaging facilities, and Prof. Jesper Wengel (Biomolecular Nanoscale Engineering Center, University of Southern Denmark) for providing the tdTomato1 siRNA.

Publisher Copyright:
© Springer Science+Business Media, LLC, part of Springer Nature 2021.

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