No linkage and association of atopy to chromosome 16 including the interleukin-4 receptor gene

A Haagerup, T Bjerke, P O Schiøtz, R Dahl, H G Binderup, T A Kruse

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

BACKGROUND: Several susceptibility genes for atopy have been suggested in recent years. Few have been investigated as intensively as the interleukin-4-receptor alpha (IL4Ralpha) gene on chromosome 16. The results remain in dispute. Therefore, in a robust design, we tested for association of type I allergy to the IL4R variations I50V and Q576R, and investigated chromosome 16 for atopy candidate regions in general.

METHODS: We identified 100 Danish allergy sib-pair families. Five conservative phenotypes for type I allergy were defined and evaluated. The IL4R variations were genotyped in trios and evaluated by the transmission disequilibrium test (TDT). Multipoint linkage analysis and exclusion mapping were conducted with sib-pairs analyzed for 17 microsatellite markers.

RESULTS: No evidence for association or linkage to the IL4R polymorphisms was found (P values: 0.12-0.90). Linkage analysis did not support linkage of any of the phenotypes to chromosome 16. Major parts of chromosome 16 were excluded as candidate regions harboring oligogenes for type I allergy.

CONCLUSION: We found chromosome 16 unlikely to harbor strong candidate genes for type I allergy. The role of the IL4Ralpha gene in the inheritance of atopy was insignificant in the Danish population. The use of conservative allergy phenotypes in the search for genes predisposing to atopic disease was discussed.

OriginalsprogEngelsk
BogserieAllergy: European Journal of Allergy and Clinical Immunology, Supplement
Vol/bind56
Udgave nummer8
Sider (fra-til)775-9
Antal sider5
ISSN0105-4538
StatusUdgivet - aug. 2001

Fingeraftryk

Interleukin-4 Receptors
Chromosomes, Human, Pair 16
Hypersensitivity
Interleukin-4 Receptor alpha Subunit
Population

Citer dette

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title = "No linkage and association of atopy to chromosome 16 including the interleukin-4 receptor gene",
abstract = "BACKGROUND: Several susceptibility genes for atopy have been suggested in recent years. Few have been investigated as intensively as the interleukin-4-receptor alpha (IL4Ralpha) gene on chromosome 16. The results remain in dispute. Therefore, in a robust design, we tested for association of type I allergy to the IL4R variations I50V and Q576R, and investigated chromosome 16 for atopy candidate regions in general.METHODS: We identified 100 Danish allergy sib-pair families. Five conservative phenotypes for type I allergy were defined and evaluated. The IL4R variations were genotyped in trios and evaluated by the transmission disequilibrium test (TDT). Multipoint linkage analysis and exclusion mapping were conducted with sib-pairs analyzed for 17 microsatellite markers.RESULTS: No evidence for association or linkage to the IL4R polymorphisms was found (P values: 0.12-0.90). Linkage analysis did not support linkage of any of the phenotypes to chromosome 16. Major parts of chromosome 16 were excluded as candidate regions harboring oligogenes for type I allergy.CONCLUSION: We found chromosome 16 unlikely to harbor strong candidate genes for type I allergy. The role of the IL4Ralpha gene in the inheritance of atopy was insignificant in the Danish population. The use of conservative allergy phenotypes in the search for genes predisposing to atopic disease was discussed.",
keywords = "Child, Chromosomes, Human, Pair 16, Female, Genetic Linkage, Genetic Variation, Humans, Hypersensitivity, Lod Score, Male, Receptors, Interleukin-4, Journal Article, Research Support, Non-U.S. Gov't",
author = "A Haagerup and T Bjerke and Schi{\o}tz, {P O} and R Dahl and Binderup, {H G} and Kruse, {T A}",
year = "2001",
month = "8",
language = "English",
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pages = "775--9",
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No linkage and association of atopy to chromosome 16 including the interleukin-4 receptor gene. / Haagerup, A; Bjerke, T; Schiøtz, P O; Dahl, R; Binderup, H G; Kruse, T A.

I: Allergy: European Journal of Allergy and Clinical Immunology, Supplement, Bind 56, Nr. 8, 08.2001, s. 775-9.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - No linkage and association of atopy to chromosome 16 including the interleukin-4 receptor gene

AU - Haagerup, A

AU - Bjerke, T

AU - Schiøtz, P O

AU - Dahl, R

AU - Binderup, H G

AU - Kruse, T A

PY - 2001/8

Y1 - 2001/8

N2 - BACKGROUND: Several susceptibility genes for atopy have been suggested in recent years. Few have been investigated as intensively as the interleukin-4-receptor alpha (IL4Ralpha) gene on chromosome 16. The results remain in dispute. Therefore, in a robust design, we tested for association of type I allergy to the IL4R variations I50V and Q576R, and investigated chromosome 16 for atopy candidate regions in general.METHODS: We identified 100 Danish allergy sib-pair families. Five conservative phenotypes for type I allergy were defined and evaluated. The IL4R variations were genotyped in trios and evaluated by the transmission disequilibrium test (TDT). Multipoint linkage analysis and exclusion mapping were conducted with sib-pairs analyzed for 17 microsatellite markers.RESULTS: No evidence for association or linkage to the IL4R polymorphisms was found (P values: 0.12-0.90). Linkage analysis did not support linkage of any of the phenotypes to chromosome 16. Major parts of chromosome 16 were excluded as candidate regions harboring oligogenes for type I allergy.CONCLUSION: We found chromosome 16 unlikely to harbor strong candidate genes for type I allergy. The role of the IL4Ralpha gene in the inheritance of atopy was insignificant in the Danish population. The use of conservative allergy phenotypes in the search for genes predisposing to atopic disease was discussed.

AB - BACKGROUND: Several susceptibility genes for atopy have been suggested in recent years. Few have been investigated as intensively as the interleukin-4-receptor alpha (IL4Ralpha) gene on chromosome 16. The results remain in dispute. Therefore, in a robust design, we tested for association of type I allergy to the IL4R variations I50V and Q576R, and investigated chromosome 16 for atopy candidate regions in general.METHODS: We identified 100 Danish allergy sib-pair families. Five conservative phenotypes for type I allergy were defined and evaluated. The IL4R variations were genotyped in trios and evaluated by the transmission disequilibrium test (TDT). Multipoint linkage analysis and exclusion mapping were conducted with sib-pairs analyzed for 17 microsatellite markers.RESULTS: No evidence for association or linkage to the IL4R polymorphisms was found (P values: 0.12-0.90). Linkage analysis did not support linkage of any of the phenotypes to chromosome 16. Major parts of chromosome 16 were excluded as candidate regions harboring oligogenes for type I allergy.CONCLUSION: We found chromosome 16 unlikely to harbor strong candidate genes for type I allergy. The role of the IL4Ralpha gene in the inheritance of atopy was insignificant in the Danish population. The use of conservative allergy phenotypes in the search for genes predisposing to atopic disease was discussed.

KW - Child

KW - Chromosomes, Human, Pair 16

KW - Female

KW - Genetic Linkage

KW - Genetic Variation

KW - Humans

KW - Hypersensitivity

KW - Lod Score

KW - Male

KW - Receptors, Interleukin-4

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

M3 - Journal article

C2 - 11488673

VL - 56

SP - 775

EP - 779

JO - Allergy: European Journal of Allergy and Clinical Immunology, Supplement

JF - Allergy: European Journal of Allergy and Clinical Immunology, Supplement

SN - 0108-1675

IS - 8

ER -