Nitrocellulose redox permanganometry: A simple method for reductive capacity assessment

Jan Homolak*, Ivan Kodvanj, Ana Babic Perhoc, Davor Virag, Ana Knezovic, Jelena Osmanovic Barilar, Peter Riederer, Melita Salkovic-Petrisic

*Kontaktforfatter

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

97 Downloads (Pure)

Abstract

We propose a rapid, simple, and robust method for measurement of the reductive capacity of liquid and solid biological samples based on potassium permanganate reduction followed by trapping of manganese dioxide precipitate on a nitrocellulose membrane. Moreover, we discuss how nitrocellulose redox permanganometry (NRP) can be used for high-throughput analysis of biological samples and present HistoNRP, its modification used for detailed analysis of reductive capacity spatial distribution in tissue with preserved anatomical relations. • NRP is a rapid, cost-effective, and simple method for reductive capacity assessment • NRP is compatible with a high-throughput screening of solid and liquid biological samples • HistoNRP exploits passive diffusion slice print blotting for reductive capacity spatial analysis

OriginalsprogEngelsk
Artikelnummer101611
TidsskriftMethodsX
Vol/bind9
ISSN2215-0161
DOI
StatusUdgivet - jan. 2022

Bibliografisk note

Funding Information:
JH conceptualized the method, designed experimental protocols and validation tests, and conducted all NRP experiments. JH, IK, and DV conducted data analysis and visualization. JH, IK, ABP, and DV wrote the manuscript. JH, ABP, AK, and JOB conducted animal experiments from which tissue samples were taken for proof-of-concept analyses. MSP is head of the Laboratory for Molecular Neuropharmacology where all experiments were conducted, principal investigator of the Croatian Science Foundation-funded project (IP-2018-01-8938) and research group leader in the Scientific Centre of Excellence for Basic, Clinical and Translational neuroscience (GA KK01.1.1.01.0007) and supervisor and mentor of the authors. PR provided valuable comments on the manuscript and the method as a renowned expert in the field of neurochemistry and a close collaborator of the group. All authors approved the manuscript and provided comments during the writing process. All data used in the preparation of the manuscript (a total of 28 datasets) can be found on the author's GitHub repository (https://github.com/janhomolak/NRP) and in the Mendeley Data repository (10.17632/fgfpzhrxjm.1). All experiments were conducted in concordance with the highest standard of animal welfare. Only certified personnel handled animals. Animal procedures were carried out at the University of Zagreb Medical School (Zagreb, Croatia) and were in compliance with current institutional, national and international (The Animal Protection Act, NN 102/17, 32/19, NN 125/13, 14/14, 92/14, 32/19), and international (Directive 2010/63/EU) guidelines governing the use of experimental animals. The experiments were approved by the national regulatory body responsible for issuing ethical approvals, the Croatian Ministry of Agriculture, and the Ethical Committee of the University of Zagreb School of Medicine. This work was funded by the Croatian Science Foundation (IP-2018-01-8938 and IP-09-2014-4639). The research was co-financed by the Scientific Centre of Excellence for Basic, Clinical, and Translational Neuroscience (project Experimental and clinical research of hypoxic-ischemic damage in the perinatal and adult brain; GA KK01.1.1.01.0007 funded by the European Union through the European Regional Development Fund).

Funding Information:
This work was funded by the Croatian Science Foundation ( IP-2018-01-8938 and IP-09-2014-4639 ). The research was co-financed by the Scientific Centre of Excellence for Basic, Clinical, and Translational Neuroscience (project Experimental and clinical research of hypoxic-ischemic damage in the perinatal and adult brain; GA KK01.1.1.01.0007 funded by the European Union through the European Regional Development Fund).

Fingeraftryk

Dyk ned i forskningsemnerne om 'Nitrocellulose redox permanganometry: A simple method for reductive capacity assessment'. Sammen danner de et unikt fingeraftryk.

Citationsformater