TY - JOUR
T1 - Natural LacI from E. coli Yields Faster Response and Higher Level of Expression than the LVA-Tagged LacI
AU - Andreassen, Patrick Rosendahl
AU - Fredberg, Sofie
AU - Horan, Mattias
AU - Knudsen, Maria Højberg
AU - Jacobsen, Kirstine
AU - Kjær, Andreas
AU - Krogh, Thøger Jensen
AU - Kronborg, Tina
AU - Mattsson, Nicky Cordua
AU - Schmidt, Sissel Ida
AU - Wille, Heidi
AU - Andersen, Ann
PY - 2014/12/19
Y1 - 2014/12/19
N2 - The lac promoter is one of the most commonly used promoters for expression control of recombinant genes in E. coli. In the absence of galactosides, the lac promoter is repressed by its repressor protein LacI. Since the lac promoter is regulated by a repressor, overexpression of LacI is necessary for regulation when the promoter is introduced on a high-copy plasmid. For that purpose, a modified variant of LacI, a LVA-tagged LacI, was submitted to the Registry of Standard Biological Parts and has been used for more than 500 constructs since then. We have found, however, that natural LacI is superior to the LVA-tagged LacI as controller of expression.
AB - The lac promoter is one of the most commonly used promoters for expression control of recombinant genes in E. coli. In the absence of galactosides, the lac promoter is repressed by its repressor protein LacI. Since the lac promoter is regulated by a repressor, overexpression of LacI is necessary for regulation when the promoter is introduced on a high-copy plasmid. For that purpose, a modified variant of LacI, a LVA-tagged LacI, was submitted to the Registry of Standard Biological Parts and has been used for more than 500 constructs since then. We have found, however, that natural LacI is superior to the LVA-tagged LacI as controller of expression.
U2 - 10.1021/sb500031h
DO - 10.1021/sb500031h
M3 - Journal article
C2 - 25524095
SN - 2161-5063
VL - 3
SP - 949
EP - 952
JO - ACS Synthetic Biology
JF - ACS Synthetic Biology
IS - 12
ER -