Natural LacI from E. coli Yields Faster Response and Higher Level of Expression than the LVA-Tagged LacI

Patrick Rosendahl Andreassen, Sofie Fredberg, Mattias Horan, Maria Højberg Knudsen, Kirstine Jacobsen, Andreas Kjær, Thøger Jensen Krogh, Tina Kronborg, Nicky Cordua Mattsson, Sissel Ida Schmidt, Heidi Wille, Ann Andersen

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Abstrakt

The lac promoter is one of the most commonly used promoters for expression control of recombinant genes in E. coli. In the absence of galactosides, the lac promoter is repressed by its repressor protein LacI. Since the lac promoter is regulated by a repressor, overexpression of LacI is necessary for regulation when the promoter is introduced on a high-copy plasmid. For that purpose, a modified variant of LacI, a LVA-tagged LacI, was submitted to the Registry of Standard Biological Parts and has been used for more than 500 constructs since then. We have found, however, that natural LacI is superior to the LVA-tagged LacI as controller of expression.

OriginalsprogEngelsk
TidsskriftA C S Synthetic Biology
Vol/bind3
Udgave nummer12
Sider (fra-til)949-952
ISSN2161-5063
DOI
StatusUdgivet - 19. dec. 2014

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Citationsformater

Andreassen, P. R., Fredberg, S., Horan, M., Knudsen, M. H., Jacobsen, K., Kjær, A., Krogh, T. J., Kronborg, T., Mattsson, N. C., Schmidt, S. I., Wille, H., & Andersen, A. (2014). Natural LacI from E. coli Yields Faster Response and Higher Level of Expression than the LVA-Tagged LacI. A C S Synthetic Biology, 3(12), 949-952. https://doi.org/10.1021/sb500031h