Abstract
Understanding the penetration properties of substances across biological bar-
riers and membranes is vital for many areas of research. In the case of human
skin, the barrier is primarily found in the stratum corneum and consists of
protein-enriched cells surrounded by a lipid membrane -enriched intercellular
spaces. Characterization of the structural and dynamical processes occurring
across the skin barrier is essential for understanding healthy and diseased
skin and for designing successful transdermal drug delivery strategies. In this
study we use Stimulated emission depletion (STED), two photon excited
STED and Forster Resonance Energy Transfer (FRET) microscopy to probe
the structure of human skin. Super resolution optical microscopy enables
resolving structures in the skin below to 60 nm allowing visualization of the
stratum corneum intercellular lipid matrix and individual proteins such as tight
junction (TJ) proteins and corneodesmosomes. To further probe the nanoscopic
structure of the intercellular lipids and the nanoscopic diffusion routes of hy-
drophilic and hydrophobic particles through the skin barrier we use FRET mea-
surements of lipophilic and hydrophilic dye pairs. The measurements are
carried out in artificial membranes of extracted human skin lipids and in skin
tissue. They show FRET distance which agree with membrane models derived
from small angle X-ray measurements of human skin lipids.
riers and membranes is vital for many areas of research. In the case of human
skin, the barrier is primarily found in the stratum corneum and consists of
protein-enriched cells surrounded by a lipid membrane -enriched intercellular
spaces. Characterization of the structural and dynamical processes occurring
across the skin barrier is essential for understanding healthy and diseased
skin and for designing successful transdermal drug delivery strategies. In this
study we use Stimulated emission depletion (STED), two photon excited
STED and Forster Resonance Energy Transfer (FRET) microscopy to probe
the structure of human skin. Super resolution optical microscopy enables
resolving structures in the skin below to 60 nm allowing visualization of the
stratum corneum intercellular lipid matrix and individual proteins such as tight
junction (TJ) proteins and corneodesmosomes. To further probe the nanoscopic
structure of the intercellular lipids and the nanoscopic diffusion routes of hy-
drophilic and hydrophobic particles through the skin barrier we use FRET mea-
surements of lipophilic and hydrophilic dye pairs. The measurements are
carried out in artificial membranes of extracted human skin lipids and in skin
tissue. They show FRET distance which agree with membrane models derived
from small angle X-ray measurements of human skin lipids.
| Originalsprog | Engelsk |
|---|---|
| Publikationsdato | 27. feb. 2016 |
| Status | Udgivet - 27. feb. 2016 |
| Begivenhed | 60th Biophysical Society Annual Meeting - Los Angeles Convention Center, Los Angeles, USA Varighed: 27. feb. 2016 → 2. mar. 2016 http://biofisica.info/60th-biophysical-society-annual-meeting/ |
Konference
| Konference | 60th Biophysical Society Annual Meeting |
|---|---|
| Lokation | Los Angeles Convention Center |
| Land/Område | USA |
| By | Los Angeles |
| Periode | 27/02/2016 → 02/03/2016 |
| Internetadresse |