Multilineage differentiation of porcine bone marrow stromal cells associated with specific gene expression pattern

Lijin Zou, Xuenong Zou, Li Chen, Haisheng Li, Tina Mygind, Moustapha Kassem, Cody Bünger

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

There are increasing reports regarding differentiation of bone marrow stromal cells (BMSC) from human and various species of animals including pigs. The phenotype and function of BMSC along a mesenchymal lineage differentiation are well characterized by specific transcription factors and marker genes. However, it is not fully clear whether multilineage differentiation (osteogenesis, chondrogenesis, and adipogenesis) of BMSC is associated with a specific gene expression pattern. In the present study, we investigated the gene expression pattern of representative transcription factors and marker genes along those three mesenchymal lineages during a particular lineage differentiation of porcine BMSC by means of real-time PCR measurement. In an osteogenic medium, the mRNA levels of cbfa1, osterix, alkaline phosphatase, type 1 collagen, osteonectin, bone sialoprotein, and osteocalcin were induced stepwise. Meanwhile, sox9, specific to chondrogenic differentiation, was inhibited but not PPARγ2 specific to adipogenic differentiation. In an adipogenic medium, adipogenic differentiation was confirmed by upregulation of PPARγ2 and aP2 and downregulation of osteogenic genes and sox9. Chondrogenic differentiation was induced in cell pellet culture by expression of sox9, type 2 collagen, and aggrecan. Cbfa1 and PPARγ2 were inhibited in chondrogenic medium. These results indicate that the differentiation potential of BMSC to a particular mesenchymal lineage relies upon specific gene expression pattern, namely upregulation of genes specific for this lineage and suppression of other lineage differentiation.
OriginalsprogEngelsk
TidsskriftJournal of Orthopaedic Research
Vol/bind26
Udgave nummer1
Sider (fra-til)56-64
ISSN0736-0266
DOI
StatusUdgivet - 2008

Fingeraftryk

Mesenchymal Stromal Cells
Up-Regulation
Osteonectin
Chondrogenesis
Adipogenesis
Collagen Type II
Osteocalcin
Collagen Type I
Osteogenesis
Alkaline Phosphatase
Real-Time Polymerase Chain Reaction
Down-Regulation
Messenger RNA

Citer dette

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title = "Multilineage differentiation of porcine bone marrow stromal cells associated with specific gene expression pattern",
abstract = "There are increasing reports regarding differentiation of bone marrow stromal cells (BMSC) from human and various species of animals including pigs. The phenotype and function of BMSC along a mesenchymal lineage differentiation are well characterized by specific transcription factors and marker genes. However, it is not fully clear whether multilineage differentiation (osteogenesis, chondrogenesis, and adipogenesis) of BMSC is associated with a specific gene expression pattern. In the present study, we investigated the gene expression pattern of representative transcription factors and marker genes along those three mesenchymal lineages during a particular lineage differentiation of porcine BMSC by means of real-time PCR measurement. In an osteogenic medium, the mRNA levels of cbfa1, osterix, alkaline phosphatase, type 1 collagen, osteonectin, bone sialoprotein, and osteocalcin were induced stepwise. Meanwhile, sox9, specific to chondrogenic differentiation, was inhibited but not PPARγ2 specific to adipogenic differentiation. In an adipogenic medium, adipogenic differentiation was confirmed by upregulation of PPARγ2 and aP2 and downregulation of osteogenic genes and sox9. Chondrogenic differentiation was induced in cell pellet culture by expression of sox9, type 2 collagen, and aggrecan. Cbfa1 and PPARγ2 were inhibited in chondrogenic medium. These results indicate that the differentiation potential of BMSC to a particular mesenchymal lineage relies upon specific gene expression pattern, namely upregulation of genes specific for this lineage and suppression of other lineage differentiation.",
keywords = "Adipocytes, Animals, Bone Marrow Cells, Cell Differentiation, Cell Lineage, Cell Separation, Cells, Cultured, Centrifugation, Density Gradient, Chondrocytes, Female, Gene Expression Profiling, Hematopoietic Stem Cells, Osteocytes, Phenotype, Reverse Transcriptase Polymerase Chain Reaction, Stromal Cells, Sus scrofa, Transcription Factors",
author = "Lijin Zou and Xuenong Zou and Li Chen and Haisheng Li and Tina Mygind and Moustapha Kassem and Cody B{\"u}nger",
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Multilineage differentiation of porcine bone marrow stromal cells associated with specific gene expression pattern. / Zou, Lijin; Zou, Xuenong; Chen, Li; Li, Haisheng; Mygind, Tina; Kassem, Moustapha; Bünger, Cody.

I: Journal of Orthopaedic Research, Bind 26, Nr. 1, 2008, s. 56-64.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Multilineage differentiation of porcine bone marrow stromal cells associated with specific gene expression pattern

AU - Zou, Lijin

AU - Zou, Xuenong

AU - Chen, Li

AU - Li, Haisheng

AU - Mygind, Tina

AU - Kassem, Moustapha

AU - Bünger, Cody

N1 - (c) 2007 Orthopaedic Research Society.

PY - 2008

Y1 - 2008

N2 - There are increasing reports regarding differentiation of bone marrow stromal cells (BMSC) from human and various species of animals including pigs. The phenotype and function of BMSC along a mesenchymal lineage differentiation are well characterized by specific transcription factors and marker genes. However, it is not fully clear whether multilineage differentiation (osteogenesis, chondrogenesis, and adipogenesis) of BMSC is associated with a specific gene expression pattern. In the present study, we investigated the gene expression pattern of representative transcription factors and marker genes along those three mesenchymal lineages during a particular lineage differentiation of porcine BMSC by means of real-time PCR measurement. In an osteogenic medium, the mRNA levels of cbfa1, osterix, alkaline phosphatase, type 1 collagen, osteonectin, bone sialoprotein, and osteocalcin were induced stepwise. Meanwhile, sox9, specific to chondrogenic differentiation, was inhibited but not PPARγ2 specific to adipogenic differentiation. In an adipogenic medium, adipogenic differentiation was confirmed by upregulation of PPARγ2 and aP2 and downregulation of osteogenic genes and sox9. Chondrogenic differentiation was induced in cell pellet culture by expression of sox9, type 2 collagen, and aggrecan. Cbfa1 and PPARγ2 were inhibited in chondrogenic medium. These results indicate that the differentiation potential of BMSC to a particular mesenchymal lineage relies upon specific gene expression pattern, namely upregulation of genes specific for this lineage and suppression of other lineage differentiation.

AB - There are increasing reports regarding differentiation of bone marrow stromal cells (BMSC) from human and various species of animals including pigs. The phenotype and function of BMSC along a mesenchymal lineage differentiation are well characterized by specific transcription factors and marker genes. However, it is not fully clear whether multilineage differentiation (osteogenesis, chondrogenesis, and adipogenesis) of BMSC is associated with a specific gene expression pattern. In the present study, we investigated the gene expression pattern of representative transcription factors and marker genes along those three mesenchymal lineages during a particular lineage differentiation of porcine BMSC by means of real-time PCR measurement. In an osteogenic medium, the mRNA levels of cbfa1, osterix, alkaline phosphatase, type 1 collagen, osteonectin, bone sialoprotein, and osteocalcin were induced stepwise. Meanwhile, sox9, specific to chondrogenic differentiation, was inhibited but not PPARγ2 specific to adipogenic differentiation. In an adipogenic medium, adipogenic differentiation was confirmed by upregulation of PPARγ2 and aP2 and downregulation of osteogenic genes and sox9. Chondrogenic differentiation was induced in cell pellet culture by expression of sox9, type 2 collagen, and aggrecan. Cbfa1 and PPARγ2 were inhibited in chondrogenic medium. These results indicate that the differentiation potential of BMSC to a particular mesenchymal lineage relies upon specific gene expression pattern, namely upregulation of genes specific for this lineage and suppression of other lineage differentiation.

KW - Adipocytes

KW - Animals

KW - Bone Marrow Cells

KW - Cell Differentiation

KW - Cell Lineage

KW - Cell Separation

KW - Cells, Cultured

KW - Centrifugation, Density Gradient

KW - Chondrocytes

KW - Female

KW - Gene Expression Profiling

KW - Hematopoietic Stem Cells

KW - Osteocytes

KW - Phenotype

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - Stromal Cells

KW - Sus scrofa

KW - Transcription Factors

U2 - 10.1002/jor.20467

DO - 10.1002/jor.20467

M3 - Journal article

VL - 26

SP - 56

EP - 64

JO - Journal of Orthopaedic Research

JF - Journal of Orthopaedic Research

SN - 0736-0266

IS - 1

ER -