Multilayer diffusion modeling and coherent anti-Stokes Raman scattering microscopy for spatially resolved water diffusion measurements in human skin

Irina Iachina, Michael A. Lomholt, Johannes H. Eriksen, Jonathan R. Brewer*

*Kontaktforfatter

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Abstract

In this work using Coherent anti-Stokes Raman Scattering microscopy, it was possible to directly measure the time dependent, spatially resolved change in concentration of water (D2O) in intact skin tissue with a spatial resolution of under 1 μm, and combined with a multilayer diffusion model, diffusion coefficients at different depths in the tissue were extracted. The results show that the diffusion varies at different layers throughout the Stratum Corneum (SC), indicating that the SC is not a homogeneous barrier but a complicated heterogeneous structure. Interestingly, averaging over the diffusion at the different depths and samples gave a relatively constant value of 0.047 ± 0.01 μm2/second. Treating the skin with acetone or tape stripping led to an increased diffusion coefficient of 0.064 ± 0.02 μm2/second and 0.079 ± 0.03 μm2/second, respectively. The combined method and model presented here shows potential for wide applications for measuring spatially resolved diffusion of different substances in a variety of different samples.

OriginalsprogEngelsk
Artikelnummere202200110
Tidsskriftjournal of biophotonics
Vol/bind15
Udgave nummer10
Antal sider10
ISSN1864-063X
DOI
StatusUdgivet - okt. 2022

Bibliografisk note

Funding Information:
This work has been supported by grants from the Leo Pharma (LF‐OC‐19‐000219) foundation and the Novo Nordisk Foundation (NNF19OC0056962). Image acquisition (and/or) image analyses were performed at the Danish Molecular Biomedical Imaging Center (DaMBIC, University of Southern Denmark), supported by the Novo Nordisk Foundation (NNF) (NNF18SA0032928).

Publisher Copyright:
© 2022 The Authors. Journal of Biophotonics published by Wiley-VCH GmbH.

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