Movement and equipositioning of plasmids by ParA filament disassembly

Simon Ringgaard, Jeroen van Zon, Martin Howard, Kenn Gerdes

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Udgivelsesdato: 2009-Nov-17
OriginalsprogEngelsk
TidsskriftProceedings of the National Academy of Science of the United States of America
Vol/bind106
Udgave nummer46
Sider (fra-til)19369-19374
Antal sider5
ISSN0027-8424
DOI
StatusUdgivet - 17. nov. 2009

Citer dette

Ringgaard, Simon ; van Zon, Jeroen ; Howard, Martin ; Gerdes, Kenn. / Movement and equipositioning of plasmids by ParA filament disassembly. I: Proceedings of the National Academy of Science of the United States of America. 2009 ; Bind 106, Nr. 46. s. 19369-19374.
@article{980994700c1711dfaefb000ea68e967b,
title = "Movement and equipositioning of plasmids by ParA filament disassembly",
abstract = "Bacterial plasmids encode partitioning (par) loci that confer stable plasmid inheritance. We showed previously that, in the presence of ParB and parC encoded by the par2 locus of plasmid pB171, ParA formed cytoskeletal-like structures that dynamically relocated over the nucleoid. Simultaneously, the par2 locus distributed plasmids regularly over the nucleoid. We show here that the dynamic ParA patterns are not simple oscillations. Rather, ParA nucleates and polymerizes in between plasmids. When a ParA assembly reaches a plasmid, the assembly reaction reverses into disassembly. Strikingly, plasmids consistently migrate behind disassembling ParA cytoskeletal structures, suggesting that ParA filaments pull plasmids by depolymerization. The perpetual cycles of ParA assembly and disassembly result in continuous relocation of plasmids, which, on time averaging, results in equidistribution of the plasmids. Mathematical modeling of ParA and plasmid dynamics support these interpretations. Mutational analysis supports a molecular mechanism in which the ParB/parC complex controls ParA filament depolymerization.",
keywords = "Amino Acid Substitution, Bacteria, Bacterial Proteins, Cell Nucleus, Green Fluorescent Proteins, Models, Molecular, Plasmids, Polymers, Thermus thermophilus",
author = "Simon Ringgaard and {van Zon}, Jeroen and Martin Howard and Kenn Gerdes",
year = "2009",
month = "11",
day = "17",
doi = "10.1073/pnas.0908347106",
language = "English",
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pages = "19369--19374",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
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publisher = "The National Academy of Sciences of the United States of America",
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Movement and equipositioning of plasmids by ParA filament disassembly. / Ringgaard, Simon; van Zon, Jeroen; Howard, Martin; Gerdes, Kenn.

I: Proceedings of the National Academy of Science of the United States of America, Bind 106, Nr. 46, 17.11.2009, s. 19369-19374.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Movement and equipositioning of plasmids by ParA filament disassembly

AU - Ringgaard, Simon

AU - van Zon, Jeroen

AU - Howard, Martin

AU - Gerdes, Kenn

PY - 2009/11/17

Y1 - 2009/11/17

N2 - Bacterial plasmids encode partitioning (par) loci that confer stable plasmid inheritance. We showed previously that, in the presence of ParB and parC encoded by the par2 locus of plasmid pB171, ParA formed cytoskeletal-like structures that dynamically relocated over the nucleoid. Simultaneously, the par2 locus distributed plasmids regularly over the nucleoid. We show here that the dynamic ParA patterns are not simple oscillations. Rather, ParA nucleates and polymerizes in between plasmids. When a ParA assembly reaches a plasmid, the assembly reaction reverses into disassembly. Strikingly, plasmids consistently migrate behind disassembling ParA cytoskeletal structures, suggesting that ParA filaments pull plasmids by depolymerization. The perpetual cycles of ParA assembly and disassembly result in continuous relocation of plasmids, which, on time averaging, results in equidistribution of the plasmids. Mathematical modeling of ParA and plasmid dynamics support these interpretations. Mutational analysis supports a molecular mechanism in which the ParB/parC complex controls ParA filament depolymerization.

AB - Bacterial plasmids encode partitioning (par) loci that confer stable plasmid inheritance. We showed previously that, in the presence of ParB and parC encoded by the par2 locus of plasmid pB171, ParA formed cytoskeletal-like structures that dynamically relocated over the nucleoid. Simultaneously, the par2 locus distributed plasmids regularly over the nucleoid. We show here that the dynamic ParA patterns are not simple oscillations. Rather, ParA nucleates and polymerizes in between plasmids. When a ParA assembly reaches a plasmid, the assembly reaction reverses into disassembly. Strikingly, plasmids consistently migrate behind disassembling ParA cytoskeletal structures, suggesting that ParA filaments pull plasmids by depolymerization. The perpetual cycles of ParA assembly and disassembly result in continuous relocation of plasmids, which, on time averaging, results in equidistribution of the plasmids. Mathematical modeling of ParA and plasmid dynamics support these interpretations. Mutational analysis supports a molecular mechanism in which the ParB/parC complex controls ParA filament depolymerization.

KW - Amino Acid Substitution

KW - Bacteria

KW - Bacterial Proteins

KW - Cell Nucleus

KW - Green Fluorescent Proteins

KW - Models, Molecular

KW - Plasmids

KW - Polymers

KW - Thermus thermophilus

U2 - 10.1073/pnas.0908347106

DO - 10.1073/pnas.0908347106

M3 - Journal article

C2 - 19906997

VL - 106

SP - 19369

EP - 19374

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 46

ER -