Molecular studies of BKCa channels in intracranial arteries: presence and localization

Helle Wulf, Anders Hay-Schmidt, Asser Nyander Poulsen, Dan Arne Klaerke, Jes Olesen, Inger Jansen-Olesen

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Udgivelsesdato: 2008-Dec
OriginalsprogEngelsk
TidsskriftCell and Tissue Research
Vol/bind334
Udgave nummer3
Sider (fra-til)359-69
Antal sider10
ISSN0302-766X
DOI
StatusUdgivet - 1. dec. 2008
Udgivet eksterntJa

Fingeraftryk

Meningeal Arteries
Messenger RNA
Real-Time Polymerase Chain Reaction
Proteins
Polymerase Chain Reaction
Middle Cerebral Artery
Vasodilation

Citer dette

Wulf, H., Hay-Schmidt, A., Poulsen, A. N., Klaerke, D. A., Olesen, J., & Jansen-Olesen, I. (2008). Molecular studies of BKCa channels in intracranial arteries: presence and localization. Cell and Tissue Research, 334(3), 359-69. https://doi.org/10.1007/s00441-008-0701-x
Wulf, Helle ; Hay-Schmidt, Anders ; Poulsen, Asser Nyander ; Klaerke, Dan Arne ; Olesen, Jes ; Jansen-Olesen, Inger. / Molecular studies of BKCa channels in intracranial arteries: presence and localization. I: Cell and Tissue Research. 2008 ; Bind 334, Nr. 3. s. 359-69.
@article{8a4a289073cd11df9ac3000ea68e967b,
title = "Molecular studies of BKCa channels in intracranial arteries: presence and localization",
abstract = "Large conductance calcium-activated potassium channels (BK(ca)) are crucial for the regulation of cerebral vascular basal tone and might be involved in cerebral vasodilation relevant to migraine and stroke. We studied the differential gene expression of mRNA transcript levels and protein expression of the BK(Ca) channel in rat basilar, middle cerebral, and middle meningeal arteries by reverse transcription polymerase chain reaction (RT-PCR), quantitative real-time PCR, and Western blotting. Distribution patterns were investigated using in situ hybridization and immunofluorescence studies. RT-PCR and quantitative real-time PCR detected the expression of the BK(Ca) channel mRNA transcript in rat basilar, middle cerebral, and middle meningeal arteries, with the transcript being expressed more abundantly in rat basilar arteries than in middle cerebral and middle meningeal arteries. Western blotting detected the BK(Ca) channel protein in rat basilar and middle cerebral arteries. In situ hybridization and immunofluorescence studies confirmed that the BK(Ca) channel mRNA and protein expression was localized to smooth muscle cells in all three intracranial arteries. The data thus suggest the presence and localization of both mRNA and protein expression of the BK(Ca) channel in the smooth muscle cell layer in rat basilar, middle cerebral, and middle meningeal arteries.",
author = "Helle Wulf and Anders Hay-Schmidt and Poulsen, {Asser Nyander} and Klaerke, {Dan Arne} and Jes Olesen and Inger Jansen-Olesen",
year = "2008",
month = "12",
day = "1",
doi = "10.1007/s00441-008-0701-x",
language = "English",
volume = "334",
pages = "359--69",
journal = "Cell and Tissue Research",
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Wulf, H, Hay-Schmidt, A, Poulsen, AN, Klaerke, DA, Olesen, J & Jansen-Olesen, I 2008, 'Molecular studies of BKCa channels in intracranial arteries: presence and localization', Cell and Tissue Research, bind 334, nr. 3, s. 359-69. https://doi.org/10.1007/s00441-008-0701-x

Molecular studies of BKCa channels in intracranial arteries: presence and localization. / Wulf, Helle; Hay-Schmidt, Anders; Poulsen, Asser Nyander; Klaerke, Dan Arne; Olesen, Jes; Jansen-Olesen, Inger.

I: Cell and Tissue Research, Bind 334, Nr. 3, 01.12.2008, s. 359-69.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Molecular studies of BKCa channels in intracranial arteries: presence and localization

AU - Wulf, Helle

AU - Hay-Schmidt, Anders

AU - Poulsen, Asser Nyander

AU - Klaerke, Dan Arne

AU - Olesen, Jes

AU - Jansen-Olesen, Inger

PY - 2008/12/1

Y1 - 2008/12/1

N2 - Large conductance calcium-activated potassium channels (BK(ca)) are crucial for the regulation of cerebral vascular basal tone and might be involved in cerebral vasodilation relevant to migraine and stroke. We studied the differential gene expression of mRNA transcript levels and protein expression of the BK(Ca) channel in rat basilar, middle cerebral, and middle meningeal arteries by reverse transcription polymerase chain reaction (RT-PCR), quantitative real-time PCR, and Western blotting. Distribution patterns were investigated using in situ hybridization and immunofluorescence studies. RT-PCR and quantitative real-time PCR detected the expression of the BK(Ca) channel mRNA transcript in rat basilar, middle cerebral, and middle meningeal arteries, with the transcript being expressed more abundantly in rat basilar arteries than in middle cerebral and middle meningeal arteries. Western blotting detected the BK(Ca) channel protein in rat basilar and middle cerebral arteries. In situ hybridization and immunofluorescence studies confirmed that the BK(Ca) channel mRNA and protein expression was localized to smooth muscle cells in all three intracranial arteries. The data thus suggest the presence and localization of both mRNA and protein expression of the BK(Ca) channel in the smooth muscle cell layer in rat basilar, middle cerebral, and middle meningeal arteries.

AB - Large conductance calcium-activated potassium channels (BK(ca)) are crucial for the regulation of cerebral vascular basal tone and might be involved in cerebral vasodilation relevant to migraine and stroke. We studied the differential gene expression of mRNA transcript levels and protein expression of the BK(Ca) channel in rat basilar, middle cerebral, and middle meningeal arteries by reverse transcription polymerase chain reaction (RT-PCR), quantitative real-time PCR, and Western blotting. Distribution patterns were investigated using in situ hybridization and immunofluorescence studies. RT-PCR and quantitative real-time PCR detected the expression of the BK(Ca) channel mRNA transcript in rat basilar, middle cerebral, and middle meningeal arteries, with the transcript being expressed more abundantly in rat basilar arteries than in middle cerebral and middle meningeal arteries. Western blotting detected the BK(Ca) channel protein in rat basilar and middle cerebral arteries. In situ hybridization and immunofluorescence studies confirmed that the BK(Ca) channel mRNA and protein expression was localized to smooth muscle cells in all three intracranial arteries. The data thus suggest the presence and localization of both mRNA and protein expression of the BK(Ca) channel in the smooth muscle cell layer in rat basilar, middle cerebral, and middle meningeal arteries.

U2 - 10.1007/s00441-008-0701-x

DO - 10.1007/s00441-008-0701-x

M3 - Journal article

C2 - 18953570

VL - 334

SP - 359

EP - 369

JO - Cell and Tissue Research

JF - Cell and Tissue Research

SN - 0302-766X

IS - 3

ER -

Wulf H, Hay-Schmidt A, Poulsen AN, Klaerke DA, Olesen J, Jansen-Olesen I. Molecular studies of BKCa channels in intracranial arteries: presence and localization. Cell and Tissue Research. 2008 dec 1;334(3):359-69. https://doi.org/10.1007/s00441-008-0701-x