MMP Mediated Degradation of Type IV Collagen Alpha 1 and Alpha 3 Chains Reflects Basement Membrane Remodeling in Experimental and Clinical Fibrosis - Validation of Two Novel Biomarker Assays

Jannie Marie Sand, Lise Skakkebæk Larsen, Cory Hogaboam, Fernando Martinez, Meilan Han, Martin Røssel Larsen, Arkadiusz Nawrocki, Qinlong Zheng, Morten Asser Karsdal, Diana Julie Leeming

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Fibrosis is characterized by excessive tissue remodeling resulting from altered expression of various growth factors, cytokines and proteases. We hypothesized that matrix metalloproteinase (MMP) mediated degradation of type IV collagen, a main component of the basement membrane, will release peptide fragments (neo-epitopes) into the circulation. Here we present the development of two competitive enzyme-linked immunosorbent assays (ELISAs) for assessing the levels of specific fragments of type IV collagen α1 (C4M12a1) and α3 (C4M12a3) chains in serum as indicators of fibrosis.
OriginalsprogEngelsk
TidsskriftPLOS ONE
Vol/bind8
Udgave nummer12
Sider (fra-til)e84934
ISSN1932-6203
DOI
StatusUdgivet - 2013

Fingeraftryk

Collagen Type IV
basement membrane
metalloproteinases
Biomarkers
Matrix Metalloproteinases
fibrosis
collagen
Assays
biomarkers
Degradation
Immunosorbents
Peptide Fragments
degradation
assays
Collagen Type I
growth factors
epitopes
Epitopes
Intercellular Signaling Peptides and Proteins
Peptide Hydrolases

Citer dette

Sand, Jannie Marie ; Larsen, Lise Skakkebæk ; Hogaboam, Cory ; Martinez, Fernando ; Han, Meilan ; Larsen, Martin Røssel ; Nawrocki, Arkadiusz ; Zheng, Qinlong ; Asser Karsdal, Morten ; Leeming, Diana Julie. / MMP Mediated Degradation of Type IV Collagen Alpha 1 and Alpha 3 Chains Reflects Basement Membrane Remodeling in Experimental and Clinical Fibrosis - Validation of Two Novel Biomarker Assays. I: PLOS ONE. 2013 ; Bind 8, Nr. 12. s. e84934.
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abstract = "Fibrosis is characterized by excessive tissue remodeling resulting from altered expression of various growth factors, cytokines and proteases. We hypothesized that matrix metalloproteinase (MMP) mediated degradation of type IV collagen, a main component of the basement membrane, will release peptide fragments (neo-epitopes) into the circulation. Here we present the development of two competitive enzyme-linked immunosorbent assays (ELISAs) for assessing the levels of specific fragments of type IV collagen α1 (C4M12a1) and α3 (C4M12a3) chains in serum as indicators of fibrosis.",
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MMP Mediated Degradation of Type IV Collagen Alpha 1 and Alpha 3 Chains Reflects Basement Membrane Remodeling in Experimental and Clinical Fibrosis - Validation of Two Novel Biomarker Assays. / Sand, Jannie Marie; Larsen, Lise Skakkebæk; Hogaboam, Cory; Martinez, Fernando; Han, Meilan; Larsen, Martin Røssel; Nawrocki, Arkadiusz; Zheng, Qinlong; Asser Karsdal, Morten; Leeming, Diana Julie.

I: PLOS ONE, Bind 8, Nr. 12, 2013, s. e84934.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - MMP Mediated Degradation of Type IV Collagen Alpha 1 and Alpha 3 Chains Reflects Basement Membrane Remodeling in Experimental and Clinical Fibrosis - Validation of Two Novel Biomarker Assays

AU - Sand, Jannie Marie

AU - Larsen, Lise Skakkebæk

AU - Hogaboam, Cory

AU - Martinez, Fernando

AU - Han, Meilan

AU - Larsen, Martin Røssel

AU - Nawrocki, Arkadiusz

AU - Zheng, Qinlong

AU - Asser Karsdal, Morten

AU - Leeming, Diana Julie

PY - 2013

Y1 - 2013

N2 - Fibrosis is characterized by excessive tissue remodeling resulting from altered expression of various growth factors, cytokines and proteases. We hypothesized that matrix metalloproteinase (MMP) mediated degradation of type IV collagen, a main component of the basement membrane, will release peptide fragments (neo-epitopes) into the circulation. Here we present the development of two competitive enzyme-linked immunosorbent assays (ELISAs) for assessing the levels of specific fragments of type IV collagen α1 (C4M12a1) and α3 (C4M12a3) chains in serum as indicators of fibrosis.

AB - Fibrosis is characterized by excessive tissue remodeling resulting from altered expression of various growth factors, cytokines and proteases. We hypothesized that matrix metalloproteinase (MMP) mediated degradation of type IV collagen, a main component of the basement membrane, will release peptide fragments (neo-epitopes) into the circulation. Here we present the development of two competitive enzyme-linked immunosorbent assays (ELISAs) for assessing the levels of specific fragments of type IV collagen α1 (C4M12a1) and α3 (C4M12a3) chains in serum as indicators of fibrosis.

U2 - 10.1371/journal.pone.0084934

DO - 10.1371/journal.pone.0084934

M3 - Journal article

C2 - 24376856

VL - 8

SP - e84934

JO - P L o S One

JF - P L o S One

SN - 1932-6203

IS - 12

ER -